Purple foliage coloration in tea (Camellia sinensis L.) arises from activation of the R2R3-MYB transcription factor CsAN1

Sun, Binmei; Zhu, Zhiwen; Cao, Panrong; Chen, Hao; Chen, Changming; Zhou, Xin; Mao, Yanhui; Liu, Jing; Jiang, Yanpin; Wei, Meng; Wang, Yingxi; Liu, Shaoqun

doi:10.1038/srep32534

Cited by 105 publications

(136 citation statements)

References 47 publications

Supporting

Mentioning

121

Contrasting

Order By: Relevance

“…Their accumulation profiles and gene expression patterns are tissue‐specific as well as development‐dependent (Jiang et al ). The higher promoter activity of CsANS observed in the meristem of the aerial parts of plants is consistent with higher gene expression and greater enzyme activity, as well as more flavonol accumulation, in young leaves (Yang et al , Zhang et al , Li et al , Sun et al , Li et al ). The activity observed in the roots may be responsible for the biosynthesis of proanthocyanidins (Fig.…”

Section: Discussionsupporting

confidence: 54%

See 1 more Smart Citation

AtHB2, a class II HD‐ZIP protein, negatively regulates the expression of CsANS, which encodes a key enzyme in Camellia sinensis catechin biosynthesis

Zhang

Jiang

et al. 2019

Physiologia Plantarum

View full text Add to dashboard Cite

Tea (Camellia sinensis) is an important cash crop that is beneficial to human health because of its remarkable content of catechins. The biosynthesis of catechins follows the flavonoid pathway, which is highly branched. Among the enzymes involved in catechin biosynthesis, ANTHOCYANIDIN SYNTHASE (CsANS) functions at a branch point and play a critical role. Our previous work has showed that the gene encoding CsANS is regulated by light signals; however, the molecular mechanism behind remains unclear. Here, we cloned a full‐length CsANS promoter and found that it contained a cis‐element recognized by Arabidopsis thaliana HOMEOBOX2 (AtHB2). AtHB2 constitutes one of the class II HOMEODOMAIN‐LEUCINE ZIPPER (HD‐ZIP) proteins, which accumulate in the dark and mediate the shade avoidance response in most angiosperms. To analyze the transcription of CsANS in vivo, β‐glucuronidase and luciferase reporter genes driven by the obtained promoter were introduced into A. thaliana and Nicotiana attenuata, respectively. In both expression systems there were indications that the A. thaliana PRODUCTION OF ANTHOCYANIN PIGMENT1 (AtPAP1), a MYB transcription factor of flavonoid biosynthesis, increased the activity of the CsANS promoter, while AtHB2 could significantly undermine the effect of AtPAP1. Yeast two‐hybrid and bimolecular fluorescence complementation assays showed that AtHB2 interacted with the A. thaliana TRANSPARENT TESTA GLABRA 1 (AtTTG1). A yeast three‐hybrid assay further suggested that AtHB2 represses the expression of CsANS and regulates its response to light signals through competitive interactions with AtTTG1. These results show that HD‐ZIP II proteins participate in light regulation of flavonoid biosynthesis.

show abstract

Section: Discussionsupporting

confidence: 54%

“…Error bars indicate the SD of three technical replicates, and the results were consistent across the three biological replicates. (Yang et al 2012, Zhang et al 2012, Li et al 2015, Sun et al 2016. The activity observed in the roots may be responsible for the biosynthesis of proanthocyanidins ( Fig.…”

Section: Discussionmentioning

confidence: 99%

AtHB2, a class II HD‐ZIP protein, negatively regulates the expression of CsANS, which encodes a key enzyme in Camellia sinensis catechin biosynthesis

Zhang

Jiang

et al. 2019

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…A tea cDNA library was constructed as previously described (Sun et al 2016). Briefly, total RNA was extracted from S1, S2, S3 and S4.…”

Section: Transcriptome Sequencing and Identification Of The Trichome mentioning

confidence: 99%

“…1A-B). The data were retrieved from a public database and were reported previously (Sun et al 2016). would be preferentially or highly expressed in young leaves. Evaluation of trichome formation and CsTTG1 expression in tea leaves at different developmental stages.…”

Section: Identification Of Csttg1 With Transcriptome Sequencingmentioning

confidence: 99%

“…Therefore, we hypothesized that the genes related to trichome formation Figure 1. To identify the genes related to trichome formation, we retrieved the transcriptome sequence data reported in our previous studies (Sun et al 2016) and reanalysed the raw data. (A) 'Zijuan' tea leaves at different developmental stages.…”

Section: Identification Of Csttg1 With Transcriptome Sequencingmentioning

confidence: 99%

See 1 more Smart Citation

TRANSPARENT TESTA GLABRA1 (TTG1) regulates leaf trichome density in tea Camellia sinensis

Sun

Zhu

Liu

et al. 2020

Nordic Journal of Botany

Self Cite

View full text Add to dashboard Cite

Plant leaf trichomes are both essential for taxonomy and participate in plant resistance to biotic and abiotic stresses. In the tea plant, teaf trichomes vary significantly among different varieties, ranging from leaves with high trichome density to relatively glabrous leaves. Leaf trichomes provides crucial diagnostic characters for tea identification and taxonomy. In addition, trichomes are a valued trait in tea production; leaf trichomes are generally considered to be associated with improved taste. However, the molecular mechanisms of trichome formation and the genetic control of trichome density in tea plants remain unknown. In this study, we identified a gene named TRANSPARENT TESTA GLABRA1 (CsTTG1), which encodes a WD‐repeat protein and is associated with trichome formation in tea. The results also showed that CsTTG1 is particularly highly expressed in the top bud, which is consistent with trichome formation in this tissue. CsTTG1 encodes a protein localized in both the nucleus and the cytoplasm. In addition, the transcription level of CsTTG1 was related to the length and/or density of the trichomes in different tea cultivars with diverse leaf trichome densities and overexpressing CsTTG1 increased the number of trichomes in Arabidopsis thaliana. Collectively, our results indicate that CsTTG1 is involved in regulating trichome formation in general and that the expression level of CsTTG1 is related to trichome density in tea plants. Our results may facilitate research on tea taxonomy and the adaptation of tea to its habitats.

show abstract

Spectroscopic studies and molecular docking on the interaction of delphinidin‐3‐O‐galactoside with tyrosinase

Chen

Shi

Liu

et al. 2021

Biotech and App Biochem

View full text Add to dashboard Cite

The inhibitory effects of delphinidin-3-O-galactoside (DG) on the activities of tyrosinase (EC 1.14.18.1) (TY) from the edible Agaricus bisporus mushroom were investigated by enzyme kinetics, multispectroscopic methods, and molecular docking. As a result, DG showed strong inhibition on TY with the IC 50 of 34.14 × 10 -6 mol L -1 . The inhibition mode of DG against TY was mixed type with α values of 5.09. The binding constant K a and related thermodynamic parameters at the three different temperatures showed that the fluorescence quenching of TY by DG was static quenching. Synchronous fluorescence, three-dimensional fluorescence, ultraviolet-visible spectroscopy, and circular dichroism spectroscopies confirmed that the conformation or microenvironment of the TY protein were changed after binding with DG. Molecular docking revealed that DG had strong binding affinity to TY through hydrogen bonding and van der Waals force, and the results were consistent with the fluorescence data. Our findings suggested that DG may be potential TY inhibitor.

show abstract

Purple foliage coloration in tea (Camellia sinensis L.) arises from activation of the R2R3-MYB transcription factor CsAN1

Cited by 105 publications

References 47 publications

AtHB2, a class II HD‐ZIP protein, negatively regulates the expression of CsANS, which encodes a key enzyme in Camellia sinensis catechin biosynthesis

AtHB2, a class II HD‐ZIP protein, negatively regulates the expression of CsANS, which encodes a key enzyme in Camellia sinensis catechin biosynthesis

TRANSPARENT TESTA GLABRA1 (TTG1) regulates leaf trichome density in tea Camellia sinensis

Spectroscopic studies and molecular docking on the interaction of delphinidin‐3‐O‐galactoside with tyrosinase

Contact Info

Product

Resources

About