Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice

Abstract

Zea mays L. (Poaceae), also known as purple corn, is an annual herbaceous plant that is grown as food for human consumption in a variety of forms, including cooking oils and sweeteners in processed food and beverage products. The purpose of this study was to determine whether a novel purple corn extract, FB801, might have an anti-atopic dermatitis (AD) effect on AD-like skin lesions induced by 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice. Topical sensitization (1%) and challenge (0.3%) by DNCB were performed on the dorsal skin and right ear of BALB/c mice to induce AD. Following FB801 and dexamethasone administered orally, the severity of skin lesions was examined macroscopically and histologically. Serum levels of immunoglobulin E (IgE) and various cytokines were determined by enzyme-linked immunosorbent assay. Oral administration of FB801 significantly reduced typical symptoms of AD (erythema/bleeding, swelling, molting/erosion and scaling/drying), scratching frequencies, and the recruitment of inflammatory and mast cells. In addition, FB801 suppressed serum levels of IgE and T helper (Th)2 type cytokines such as interleukin (IL)-4 and IL-10 in DNCB-treated BALB/c mice. Furthermore, FB801 reduced the degradation of inhibitor of nuclear factor-κB proteins (NF-κB) in tumor necrosis factor (TNF)-α-stimulated human keratinocyte (HaCaT) cells. These results suggest that FB801 inhibited the development of the AD-like skin symptoms by regulating Th1 and Th2 responses in the skin lesions in mice and suppressing TNF-α induced NF-κB activation in HaCaT cells, suggesting that FB801 has potential application as an effective alternative therapy for the prevention and management of AD.

Anti-Atopic Activities of Sargassum horneri Hot Water Extracts in 2,4-Dinitrochlorobezene-Induced Mouse Models

“…The Th2 cytokines IL-5 and IL-4 take an role in AD by increasing IgE production and eosinophils [ 3 ]. The alleviating effect of red corn extract on AD in DNCB-induced mice [ 5 ] and the anti-atopic activity of ethanol extract from tuna heart [ 6 ] that produce decreased IFN-γ and enhanced proportion of allergen-specific T cells associated with increased IL-5 and IL-4 have been reported in peripheral blood smears of AD patients. The cause of atopy is not yet known, but genetic, immunological , and environmental factors are known to predispose patients to this disorder [ 7 ].…”

Chemical fluorescence-based dye staining for 3-dimensional histopathology analysis

“…As normal histopathological tests often involve general staining such as hematoxylin–eosin (H&E) staining (No et al 2021 ; Roh et al 2021 ), the development of a fluorescence staining equivalent to H&E staining is important. Eosin labels cytosol in red and exhibits red fluorescence via the conventional rhodamine filter setup of a fluorescence microscope.…”