Purple acid phosphatase 10c encodes a major acid phosphatase that regulates plant growth under phosphate-deficient conditions in rice

Abstract

<jats:title>Abstract</jats:title> <jats:p>Whilst constitutive overexpression of particular acid phosphatases (APases) can increase utilization of extracellular organic phosphate, negative effects are frequently observed in these transgenic plants under conditions of inorganic phosphate (Pi) sufficiency. In this study, we identified rice purple acid phosphatase 10c (OsPAP10c) as being a novel and major APase that exhibits activities associated both with the root surface and with secretion. Two constructs were used to generate the OsPAP10c-overexpression plants by driving its coding sequence with either a ubiquitin promoter (UP) or the OsPAP10c-native promoter (NP). Compared with the UP transgenic plants, lower expression levels and APase activities were observed in the NP plants. However, the UP and NP plants both showed a similar ability to degrade extracellular ATP and both promoted root growth. The growth performance and yield of the NP transgenic plants were better than the wild-type and UP plants in both hydroponic and field experiments irrespective of the level of Pi supply. Overexpression of APase by its native promoter therefore provides a potential way to improve crop production that might avoid increased APase activity in untargeted tissues and its inhibition of the growth of transgenic plants.</jats:p>

Genome-wide analysis of haloacid dehalogenase genes reveals their function in phosphate starvation responses in rice

“…It has been reported that plants synthesize intercellular and extracellular phosphatases to recycle different Po under Pi starvation conditions [ 16 ]. The majority of the Pi starvation-induced secreted acid phosphatases in plants are PAPs, which belong to a unique acid phosphatase subfamily.…”

“…In contrast, the activity of secreted acid phosphatases (SAPs) is enhanced under prolonged Pi starvation conditions and participates in the degradation of extracellular Po [ 6 , 8 , 9 ]. Many Pi starvation-induced IAPs and SAPs have been purified from different plant species, and the majority of them are encoded by purple acid phosphatase (PAP) genes [ 8 , 10 – 16 ]. Compared with PAP genes, other types of Pi starvation-induced phosphatases are less studied in plants.…”

Genome-Wide Characterization and Evolutionary Analyses of Purple Acid Phosphatase (PAP) Gene Family with Their Expression Profiles in Response to Low Phosphorus Stresses in Moso Bamboo (Phyllostachys edulis)

“…Many plant-derived PAPs have acid phosphatase activity, which can transform organic phosphorus in and out of plants into the phosphorus that is accessible for the plant for plants to absorb and utilize [20]. It is reported that the activity of acid phosphatase in rice can be significantly enhanced under low phosphorus induction, which can promote the absorption and utilization of phosphorus and then affect the plant biomass [12]. Interestingly, in our study, low phosphorus also induced the increase in acid phosphatase activity in the root, stem and leaf organs of Moso bamboo, but with the increase in low phosphorus treatment time, the acid phosphatase activity in stems and leaves decreased significantly.…”

“…Root length was measured by a root scanning analysis system (WinRHIZ0). According to the test method of related researchers [12], p-nitrophosphate phenol (pNPP) was measured to quantify the secretory acid phosphatase activity associated with roots. Roots and leaves excised from seedlings grown in normal phosphorus and low phosphorus nutrient solution for 0 h, 48 h, and 96 h were washed with double distilled water.…”

“…In the long-term evolution of plants, a series of morphological and physiological adaptation mechanisms have evolved to cope with a low phosphorus stress environment. Low phosphorus can induce a series of endogenous hormone secretion, acid phosphatase secretion [12], organic acid metabolism enhancement [13] and the expression of related genes [14], thereby promoting root elongation, organic acid and proton secretion [15]. These low phosphorus responses and adaptation mechanisms activate the phosphorus in the rhizosphere soil for plant utilization, enhance the root system's phosphorus-seeking ability and enhance the plant's phosphorus reuse efficiency [16].…”