Purmorphamine increased adhesion, proliferation and expression of osteoblast phenotype markers of human dental pulp stem cells cultured on beta-tricalcium phosphate

Rad, Maryam Rezia; Khojaste, Moein; Shahriari, Mehrnoosh Hasan; Asgary, Saeed; Khojasteh, Arash

doi:10.1016/j.biopha.2016.05.016

Cited by 14 publications

(12 citation statements)

References 30 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Beloti's study, 40 it was found that purmorphamine did not affect the proliferation of human bone marrow mesenchymal cells and osteoblast differentiation but that it increased ALP expression and the ability of cells to produce calcium nodules. However, Rezia and others 41 found that the addition of purmorphamine could promote the proliferation of hDPSCs, consistent with the results of this experiment.…”

Section: Suppressing Of Stathmin In Hdpscs Inhibits Proliferationsupporting

confidence: 91%

Stathmin inhibits proliferation and differentiation of dental pulp stem cells via sonic hedgehog/Gli

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

The mineralization of dental pulp stem cells is an important factor in the tissue engineering of teeth, but the mechanism is not yet obvious. This study aimed to identify the effect of Stathmin on the proliferation and osteogenic/odontoblastic differentiation of human dental pulp stem cells (hDPSCs) and to explore whether the Shh signalling pathway was involved in this regulation. First, Stathmin was expressed in the cytoplasm and on the cell membranes of hDPSCs by cell immunofluorescence. Then, by constructing a lentiviral vector, the expression of Stathmin in hDPSCs was inhibited. Treatment with Stathmin shRNA (shRNA‐Stathmin group) inhibited the ability of hDPSCs to proliferate, as demonstrated by a CCK8 assay and flow cytometry analysis, and suppressed the osteogenic/odontoblastic differentiation ability, as demonstrated by alizarin red S staining and osteogenic/odontoblastic differentiation‐related gene (ALP, BSP, OCN, DSPP) activity, compared to that of hDPSCs from the control shRNA group. Molecular analyses showed that the Shh/GLI1 signalling pathway was inhibited when Stathmin was silenced, and purmorphamine, the Shh signalling pathway activator, was added to hDPSCs in the shRNA‐Stathmin group, real‐time PCR and Western blotting confirmed that expression of Shh and its downstream signalling molecules PTCH1, SMO and GLI1 increased significantly. After activating the Shh signalling pathway, the proliferation of hDPSCs increased markedly, as demonstrated by a CCK8 assay and flow cytometry analysis; osteogenic/odontoblastic differentiation‐related gene (ALP, BSP, OCN, DSPP) expression also increased significantly. Collectively, these findings firstly revealed that Stathmin‐Shh/GLI1 signalling pathway plays a positive role in hDPSC proliferation and osteogenic/odontoblastic differentiation.

show abstract

Section: Suppressing Of Stathmin In Hdpscs Inhibits Proliferationsupporting

confidence: 91%

Stathmin inhibits proliferation and differentiation of dental pulp stem cells via sonic hedgehog/Gli

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Its main component, β-LG, has an affinity for hydrophobic molecules, which are poorly soluble in water, and β-LG can be used as a carrier protein to improve the solubility and bioavailability of hydrophobic molecules. Thus, β-LG could be used as a carrier or delivery protein for certain molecules that promote osteogenic differentiation, such as purmorphamine (Rezia Rad et al, 2016) or that are thought to promote bone healing, such as vitamin D (Gorter et al, 2014). In addition, WPI can be used to fabricate hydrogels (Puyol et al, 2001), which are gaining interest as biomaterials for bone regeneration (Gkioni et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells

Douglas

Vandrovcová

Kročilová

et al. 2018

Journal of Dairy Science

View full text Add to dashboard Cite

Recently, milk-derived proteins have attracted attention for applications in the biomedical field such as tissue regeneration. Whey protein isolate (WPI), especially its main component β-lactoglobulin, can modulate immunity and acts as an antioxidant, antitumor, antiviral, and antibacterial agent. There are very few reports of the application of WPI in tissue engineering, especially in bone tissue engineering. In this study, we tested the influence of different concentrations of WPI on behavior of human osteoblast-like Saos-2 cells, human adipose tissue-derived stem cells (ASC), and human neonatal dermal fibroblasts (FIB). The positive effect on growth was apparent for Saos-2 cells and FIB but not for ASC. However, the expression of markers characteristic for early osteogenic cell differentiation [type-I collagen (COL1) and alkaline phosphatase (ALP)] as well as ALP activity, increased dose-dependently in ASC. Importantly, Saos-2 cells were able to deposit calcium in the presence of WPI, even in a proliferation medium without other supplements that support osteogenic cell differentiation. The results indicate that, depending on the cell type, WPI can act as an enhancer of cell proliferation and osteogenic differentiation. Therefore, enrichment of biomaterials for bone regeneration with WPI seems a promising approach, especially due to the low cost of WPI.

show abstract

“…DNA quantification was carried out using the salting-out method, as described previously. 55 Briefly, decellularized scaffolds were lyzed using TRIzol solution for 10 min. Next, phase separation was conducted by addition of chloroform and centrifugation of solution at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…DNA quantification was carried out using the salting-out method, as described previously . Briefly, decellularized scaffolds were lyzed using TRIzol solution for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of Decellularized Engineered Extracellular Matrix through Bioreactor-Based Environment for Bone Tissue Engineering

et al. 2020

Self Cite

View full text Add to dashboard Cite

Extracellular matrix (ECM)-contained grafts can be achieved by decellularization of native bones or synthetic scaffolds. Limitations associated with harvesting the native bone has raised interest in preparing in vitro ECM bioscaffold for bone tissue engineering. Here, we intend to develop an ECM-contained construct via decellularizing an engineered gelatin-coated β-tricalcium phosphate (gTCP) scaffold. In order to find an optimal protocol for decellularization of cell-loaded gTCP scaffolds, they were seeded with buccal fat pad-derived stem cells. Then, four decellularization protocols including sodium dodecyl sulfate, trypsin, Triton X-100, and combined solution methods were compared for the amounts of residual cells and remnant collagen and alteration of scaffold structure. Then, the efficacy of the selected protocol in removing cells from gTCP scaffolds incubated in a rotating and perfusion bioreactor for 24 days was evaluated and compared with static condition using histological analysis. Finally, decellularized scaffolds, reloaded with cells, and their cytotoxicity and osteoinductive capability were evaluated. Complete removal of cells from gTCP scaffolds was achieved from all protocols. However, treatment with Triton X-100 showed significantly higher amount of remnant ECM. Bioreactor-incubated scaffolds possessed greater magnitude of ECM proteins including collagen and glycosaminoglycans. Reseeding the decellularized scaffolds also represented higher osteoinductivity of bioreactor-based scaffolds. Application of Triton X-100 as decellularization protocol and usage of bioreactors are suggested as a suitable technique for designing ECM-contained grafts for bone tissue engineering.

show abstract

Purmorphamine increased adhesion, proliferation and expression of osteoblast phenotype markers of human dental pulp stem cells cultured on beta-tricalcium phosphate

Cited by 14 publications

References 30 publications

Stathmin inhibits proliferation and differentiation of dental pulp stem cells via sonic hedgehog/Gli

Stathmin inhibits proliferation and differentiation of dental pulp stem cells via sonic hedgehog/Gli

Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells

Fabrication of Decellularized Engineered Extracellular Matrix through Bioreactor-Based Environment for Bone Tissue Engineering

Contact Info

Product

Resources

About