Purkinje cell phenotype restricts the distribution of unipolar brush cells

Chung, Siu-Leung; Sillitoe, Roy V.; Croci, Laura; Badaloni, Aurora; Consalez, G. Giacomo; Hawkes, Richard

doi:10.1016/j.neuroscience.2009.09.080

Cited by 35 publications

(40 citation statements)

References 70 publications

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“…Detailed specifications of these antibodies are listed in Table 1. Specificity of antibodies to CR, DGKβ, mGluR1α, and PLCβ4 has been validated previously (Shigemoto et al 1997; Nunzi et al 2002; Nakamura et al 2004; Sarna et al 2006; Hozumi et al 2008; Chung et al 2009a, b; Hozumi et al 2009). Specificity of Santa Cruz PLCβ1 and PLCβ3 antibodies was validated by Western blot analysis.…”

Section: Methodsmentioning

confidence: 95%

“…A recent study revisited the chemical phenotypes of the UBC subclasses by performing cytochemical analysis using an antibody to PLCβ4 paired with either anti-mGluR1α or anti-CR antibodies, and suggested a more complex subclass repartition within the adult, murine, cerebellar UBC population (Chung et al 2009a, b). The study proposed an essentially tripartite division, each of the subclasses representing approximately one third of the entire population: one subclass appeared mGluR1α + /PLCβ4 + /CR - , a second subclass PLCβ4 + /mGluR1α - /CR - , and a third subclass CR + /mGluR1α - /PLCβ4 - .…”

Section: Introductionmentioning

confidence: 99%

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Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes

et al. 2013

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Sublineage diversification of specific neural cell classes occurs in complex as well as simply organized regions of the central and peripheral nervous systems; the significance of the phenomenon, however, remains insufficiently understood. The unipolar brush cells (UBCs) are glutamatergic cerebellar interneurons that occur at high density in vestibulocerebellum. As they are classified into subsets that differ in chemical phenotypes, intrinsic properties, and lobular distribution, they represent a valuable neuronal model to study subclass diversification. In this study we show that cerebellar UBCs of adult rats and mice form two subclasses – type I and type II UBCs - defined by somatodendritic expression of calretinin (CR), mGluR1α, phospholipases PLCβ1 and PLCβ4, and diacylglycerol kinase-beta (DGKβ). We demonstrate that PLCβ1 is associated only with the CR+ type I UBCs while PLCβ4 and DGKβ are exclusively present in mGluR1α+ type II UBCs. Notably, all PLCβ4+ UBCs, representing about 2/3 of entire UBC population, also express mGluR1α. Furthermore, our data show that the sum of CR+ type I UBCs and mGluR1α+ type II UBCs accounts for the entire UBC class identified with Tbr2 immunolabeling. The two UBC subtypes also show a very different albeit somehow overlapping topographical distribution as illustrated by detailed cerebellar maps in this study. Our data not only complement and extend the previous knowledge on the diversity and subclass specificity of the chemical phenotypes within the UBC population but provide a new angle to the understanding of the signaling networks in type I and type II UBCs.

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Section: Methodsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Differential distribution of phospholipase C beta isoforms and diaglycerol kinase-beta in rodents cerebella corroborates the division of unipolar brush cells into two major subtypes

et al. 2013

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“…In the central nervous system, Ebf2 is expressed in the first differentiating neurons migrating from the VZ along the spinal cord to the midbrain and at specific sites in the forebrain of mouse embryos [20,29,31,32,33]. Previous studies have indicated that mutation of Ebf2 leads to a variety of nervous system defects, such as peripheral nerve abnormalities, migration defects of neurons that produce gonadotropin-releasing hormone, failed projections of olfactory neurons and abnormal development of cerebellar neurons [28,29,33,34,35], indicating that Ebf2 is important for specific aspects of neuronal development. In the forebrain, in situ hybridization experiments from early studies show that Ebf2 is widely expressed in early postmitotic neurons in regions including the septum, hippocampus and neocortex [20].…”

Section: Introductionmentioning

confidence: 99%

Ebf2 Marks Early Cortical Neurogenesis and Regulates the Generation of Cajal-Retzius Neurons in the Developing Cerebral Cortex

Chuang¹,

Wang

et al. 2011

Dev Neurosci

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Mammalian cortical neurogenesis occurs on a precise time schedule during development. The earliest born neurons form the preplate and later separate into layer 1, which includes Cajal-Retzius (C-R) neurons, and the subplate. The preplate and its derivatives play a critical role in regulating subsequent neuron migration and cortical lamination. Using an early B cell factor 2 (Ebf2)-enhanced green fluorescent protein (EGFP) transgenic mouse line, we show that Ebf2-EGFP is expressed in the preplate and persists in C-R neurons, allowing us to follow the development of these early born neurons. Therefore, Ebf2 is a genetic marker for preplate neurons from the earliest stage of their differentiation and C-R cells after the preplate is split. Additionally, we examined the function of Ebf2 in the development of C-R neurons using both lentiviral-mediated knock-down overexpression approaches to perturb Ebf2 expression. Our data show that Ebf2 overexpression increases the generation of early born neurons including C-R cells, while Ebf2 knock-down decreases it, without affecting the generation of other layer-specific neurons in vitro. These results indicate that Ebf2 is important for early cortical neurogenesis and regulates the generation of C-R neurons in the developing cerebral cortex.

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“…During postnatal development, mossy fibers move to the granular layer but stay aligned with the Purkinje cell stripe (e.g., Arsénio Nunes and Sotelo, 1985; Ji and Hawkes, 1995). A similar mechanism seems to serve to guide cerebellar interneurons to their specific stripe locations (unipolar brush cells—Chung et al, 2009a,b; Golgi cells—Sillitoe et al, 2008) and boundaries between stripes restrict the mediolateral spread of Golgi cell dendritic arbors (Sillitoe et al, 2008). …”

Section: Purkinje Cell Architecture As a Scaffold For Cerebellar Topomentioning

confidence: 99%

“…We also focus this review on patterning of Purkinje cells—less is known of the development of patterns in either granule cells or inhibitory interneurons although many of these are thought to be secondary to the patterning of the Purkinje cells (e.g., Sotelo and Chédotal, 2005; Sillitoe et al, 2008, 2010; Chung et al, 2009a,b). …”

Section: Introduction—patterning Of the Adult Cerebellummentioning

confidence: 99%