Purinoceptor expression in hepatocellular virus (HCV)-induced and non-HCV hepatocellular carcinoma: an insight into the proviral role of the P2X4 receptor

Khalid, Madiha; Manzoor, Sobia; Ahmad, Hassam; Asif, Arun; Bangash, Tariq Ali; Latif, Amer; Jaleel, Shahla

doi:10.1007/s11033-018-4432-0

Cited by 24 publications

(17 citation statements)

References 43 publications

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“…In contrary, HCC exhibited significantly high P2X7 ratios when compared with ADC and AMC tissue samples. Recent evidence suggests that higher P2X4 expression in HCC is also influenced by viral invasion [35]. HCC tissue samples also exhibited the highest expression of P2X7 receptor among all three carcinoma samples.…”

Section: Comparative Analysis Of P2x4 and P2x7 In Hepatocellular Carcmentioning

confidence: 79%

Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: an approach towards proinflammatory role of P2X4 and P2X7 receptors

Asif

Khalid

Manzoor

et al. 2019

Purinergic Signalling

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The primary malignancy of liver, known as hepatocellular carcinoma (HCC), comprises 9% of all hepatobiliary carcinomas. A steady rise has also been observed in adenocarcinoma (ADC) of the liver and ampullary carcinoma (AMC), ascending to 0.5% of gastrointestinal malignancies. Hepatobiliary carcinomas consist of 13% of all cancer occurrences worldwide. Purinergic receptor-based signaling holds the therapeutic potential based on its role in cell proliferation of several carcinomas. An altered ATP concentration in nanomoles may lead towards crucial changes in cancer growth patterns in liver tissue. A total of 40 tissue samples were collected (20 samples of HCC, 10 samples of ADC, and 10 samples of AMC) from patients that underwent surgery. P2X4 and P2X7 receptors exhibited significantly increased expression in HCC, ADC, and AMC samples as compared with the control tissue samples. While ADC and AMC samples showed higher expression of P2X4 and P2X7 than the control, statistically, HCC samples exhibited the most significant expression of both P2X4 and P2X7 receptors than control tissues. It may be inferred that higher expression of P2X4 and P2X7 receptors is significantly associated with the upregulated cellular stress leading to inflammation and it is plausible that both these receptors may be used in diagnostic, prognostic, and therapeutic tools for carcinoma studies in the future.

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Section: Comparative Analysis Of P2x4 and P2x7 In Hepatocellular Carcmentioning

confidence: 79%

Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: an approach towards proinflammatory role of P2X4 and P2X7 receptors

Asif

Khalid

Manzoor

et al. 2019

Purinergic Signalling

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“…Continuous evidence has suggested that oxidative stress leads to the buildup of hepatic inflammation [25]. One of the major biomarkers for the pro-inflammatory environment is IL-1β, which contributes to oxidative stress, subsequently leading to inflammation, which further develops into hepatocellular carcinoma [26][27][28][29]. Furthermore, continuous evidence has supported the higher expression of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) to monitor liver inflammation [30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Kim,

Asif,

Chethikkattuveli Salih

et al. 2021

Biomedicines

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The spheroid culture system provides an efficient method to emulate organ-specific pathophysiology, overcoming the traditional two-dimensional (2D) cell culture limitations. The intervention of microfluidics in the spheroid culture platform has the potential to enhance the capacity of in vitro microphysiological tissues for disease modeling. Conventionally, spheroid culture is carried out in static conditions, making the media nutrient-deficient around the spheroid periphery. The current approach tries to enhance the capacity of the spheroid culture platform by integrating the perfusion channel for dynamic culture conditions. A pro-inflammatory hepatic model was emulated using a coculture of HepG2 cell line, fibroblasts, and endothelial cells for validating the spheroid culture plate with a perfusable channel across the spheroid well. Enhanced proliferation and metabolic capacity of the microphysiological model were observed and further validated by metabolic assays. A comparative analysis of static and dynamic conditions validated the advantage of spheroid culture with dynamic media flow. Hepatic spheroids were found to have improved proliferation in dynamic flow conditions as compared to the static culture platform. The perfusable culture system for spheroids is more physiologically relevant as compared to the static spheroid culture system for disease and drug analysis.

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“…However, it remains to be elucidated whether the expression of purinergic receptors is associated with an antiviral immunity or participates in the pathophysiology of the disease [ 172 , 173 ]. Furthermore, in patients that develop hepatocellular carcinoma (HCC) induced by a HCV infection, the P2X4R expression is increased compared to patients with HCC from non-viral causes [ 174 ]. Regarding the ectoenzymes, recently a population of CD39 + Treg cells was identified to be associated with potent immunosuppressive activity and the progression of viral infections.…”

Section: Viral Infectionsmentioning

confidence: 99%

Purinergic modulation of the immune response to infections

Eberhardt

Bergero

Mariotta

et al. 2022

Purinergic Signalling

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Infectious diseases are caused by the invasion of pathogenic microorganisms such as fungi, bacteria, viruses, and parasites. After infection, disease progression relies on the complex interplay between the host immune response and the microorganism evasion strategies. The host’s survival depends on its ability to mount an efficient protective anti-microbial response to accomplish pathogen clearance while simultaneously preventing tissue injury by keeping under control the excessive inflammatory process. The purinergic system has the dual function of regulating the immune response and triggering effector antimicrobial mechanisms. This review provides an overview of the current knowledge of the modulation of innate and adaptive immunity driven by the purinergic system during parasitic, bacterial and viral infections.

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Purinoceptor expression in hepatocellular virus (HCV)-induced and non-HCV hepatocellular carcinoma: an insight into the proviral role of the P2X4 receptor

Cited by 24 publications

References 43 publications

Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: an approach towards proinflammatory role of P2X4 and P2X7 receptors

Role of purinergic receptors in hepatobiliary carcinoma in Pakistani population: an approach towards proinflammatory role of P2X4 and P2X7 receptors

Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation

Purinergic modulation of the immune response to infections

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