Purine nucleotide cycle. Evidence for the occurrence of the cycle in brain.

Schultz, Vera; Lowenstein, John M.

doi:10.1016/s0021-9258(17)33905-4

Cited by 109 publications

(3 citation statements)

References 29 publications

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“…Enzyme Assays. Adenylosuccinase was assayed as described previously (Schultz & Lowenstein, 1976). Reaction mixtures contained 20 mM potassium phosphate, pH 7.4, adenylosuccinate, and enzyme in a volume of 0.52 mL.…”

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Inhibition of adenylosuccinase by adenylophosphonopropionate and related compounds

Brand¹,

Lowenstein²

1978

Biochemistry

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Adenylosuccinase from muscle, liver and yeast is strongly inhibited by the substrate analogue adenylophosphonopropionate (N6-(DL-1-carboxy-2-phosphonoethyl)-adenosine 5'-monophosphate). The inhibition is freely reversible and of the competitive type, with apparent K1 values between 5.4 and 86 nM depending on the source of enzyme. Ratios of Km/K1 with adenylosuccinate as substrate fall in the range of 44 to 1350. Comparison of four carboxyl analogues of adenylosuccinate with the corresponding phosphonate analogues shows that the phosphonates are much better inhibitors. Adenylosuccinate analogues in which the beta-carboxyl is replaced by other functional groups are much poorer inhibitors. The exceptionally high affinity of adenylosuccinase for adenylophosphonopropionate appears to involve the dianion of the phosphonate group.

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“…The following enzymes were assayed according to published procedures: aspartate aminotransferase (Sizer & Jenkins. 1962), aspartase (Williams & Lartique, 1967), alanine dehydrogenase (Yoshida & Freese, 1964), glutamate dehydrogenase (Schultz & Lowenstein, 1976), malate dehydrogenase (Kitto, 1969), and malic enzyme (Ochoa, 1955).…”

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confidence: 99%

Inhibition of adenylosuccinase by adenylophosphonopropionate and related compounds

Brand¹,

Lowenstein²

1978

Biochemistry

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“…A similar rate of ammonia production as observed during one leg knee-extensor exercise in chapter 2 was also observed during prolonged two legged cycling exercise at 70% VC>2max . The Vmax measured /n w'fro of adenylosuccinate synthase and adenyloscuccinase in rat hindlimb are about 0.7 and 0.5 mmol.kg%et weight.min' respectively (Schulz & Lowenstein, 1976;Goodman & Lowenstein, 1977). Thus the rate of ammonia production and the activity of the rate limiting enzyme of the PN-cycle are more than sufficient to account for the rate of ammonia production.…”

Section: Muscle Ammonia Production During Prolonged Exercisementioning

confidence: 95%

Amino acids, ammonia and exercise in man

Hall¹

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Prenatal diagnosis in adenylosuccinate lyase deficiency

et al. 2000

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Adenylosuccinate lyase deficiency, an autosomal recessive inborn error of purine synthesis, provokes accumulation in body fluids of succinylaminoimidazolecarboxamide riboside and succinyladenosine, the dephosphorylated derivatives of the two substrates of the enzyme. Most patients display severe psychomotor retardation, often accompanied by epilepsy and/or autistic features, although some are only mildly retarded. About 20 mutations are known. Prenatal diagnosis was performed twice on chorion villi of the mother of a previously diagnosed patient with a C5T mutation (exon 1) on the maternal allele, and a C1185A mutation (exon 11) on the paternal allele. Both suppress a Fnu4HI restriction site. In a first fetus, incubation of PCR products generated from genomic DNA of exon 1 with Fnu4HI yielded a 113 bp fragment from a control and the father's gene, and both a 113 bp and 170 bp fragment from the mother, affected sibling and fetus. Incubation of PCR products of exons 11-12 with Fnu4HI yielded a 550 bp fragment from a control and the mother's gene, and a 550 bp and 600 bp fragment from the father, affected sibling and fetus. Assay of adenylosuccinate lyase on the aborted fetal liver confirmed the enzyme deficiency. A second fetus displayed only the maternal mutation.

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Purine nucleotide cycle. Evidence for the occurrence of the cycle in brain.

Cited by 109 publications

References 29 publications

Inhibition of adenylosuccinase by adenylophosphonopropionate and related compounds

Inhibition of adenylosuccinase by adenylophosphonopropionate and related compounds

Amino acids, ammonia and exercise in man

Prenatal diagnosis in adenylosuccinate lyase deficiency

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