Purine Gene Organization and Regulation in Bacillus Subtiles

Nygaard, Per; Duckert, P.; Saxild, Hans Henrik

doi:10.1016/b978-0-12-274161-6.50014-0

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…In both B. subtilis (Momose et al, 1966;Nygaard et al, 1988) and enteric bacteria (Neuhard & Nygaard, 1987) repressed levels of the purine biosynthetic enzymes are found when purine bases or nucleosides are present in the growth medium. Under these conditions purine synthesis de novo is reduced, and the preformed purines added to the growth medium are utilized for purine nucleotide synthesis.…”

Section: -6899mentioning

confidence: 99%

Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools

Saxild¹,

Nygaard²

1991

Journal of General Microbiology

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#The genes encoding the enzymes of IMP biosynthesis in Bacillus subtilis constitute the pur operon, whereas the genes encoding GMP biosynthetic enzymes, guuA (GMP synthetase) and guaB (IMP dehydrogenase), and the purA gene encoding adenylosuccinate (sAMP) synthetase all occur as single units. The purB gene encodes an enzyme involved in both IMP and AMP biosynthesis and is located in the pur operon. The levels of purine biosynthetic enzymes (except for GMP synthetase) were repressed in cells grown in the presence of purine compounds. Transcription of the pur operon is regulated negatively by adenine and guanine compounds. Our results suggest that ATP and guanine (or hypoxanthine) act as low molecular mass repressors. The level of IMP dehydrogenase was repressed by guanosine, but not in the presence of adenine, and was negatively correlated with the GTP/ATP pools ratio. The level of sAMP synthetase was repressed by adenine and increased by guanosine, and was positively correlated with the GTP/ATP pools ratio. It appears that the mode of regulating purine biosynthetic enzyme levels coincides with the cellular need for the individual enzymes.

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Section: -6899mentioning

confidence: 99%

Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools

Saxild¹,

Nygaard²

1991

Journal of General Microbiology

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Computerized genetic map of Bacillus subtilis

et al. 1996

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The ampS-nprE (124 -127 ) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area

Winters

Caldwell²,

Enfield³

et al. 1996

Microbiology

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A stretch of DNA approximately 27 kb in length, adjacent to the nprE gene of Bacillus subtilis, has been sequenced. The sequenced fragment carries a total of 23 ORFs. Of these, 15 could be assigned probable functions based on homologies to characterized genes either in B. subtilis or in other organisms. The sequencing of this region has also allowed us to assign to this area adeC and strB, previously located on the other side of nprE, between nprE and the pyr operon.

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Purine Gene Organization and Regulation in Bacillus Subtiles

Cited by 5 publications

References 11 publications

Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools

Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools

Computerized genetic map of Bacillus subtilis

The ampS-nprE (124 -127 ) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area

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