Purifying sugar beet pectins from non-pectic components by means of metal precipitation

Guo, Xiaoming; Meng, Hecheng; Zhu, Siming; Zhang, Tao; Yu, Shujuan

doi:10.1016/j.foodhyd.2015.05.009

Cited by 31 publications

(9 citation statements)

References 41 publications

(3 reference statements)

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“…As shown in Table 1, a significant difference was found in the Cor cultivar. The results obtained in this investigation were lower than the reported on SBP, submitted to a similar extraction process, obtaining an ash value of 3.8% [46]. The marked difference between the reported study and this work may lie in the purification process carried out in this investigation to reduce the non-pectin compounds like ash, oxalate salts, protein, and neutral sugars, which can interfere with the gelling process.…”

Section: Pectin Compositioncontrasting

confidence: 80%

Composition, Physicochemical Features, and Covalent Gelling Properties of Ferulated Pectin Extracted from Three Sugar Beet (Beta vulgaris L.) Cultivars Grown under Desertic Conditions

Lara-Espinoza¹,

Sánchez-Villegas²,

López‐Franco³

et al. 2020

Agronomy

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Sugar beet is a potential source of pectin, competitive with traditional sources, that has recently acquired great relevance for its interesting covalent gelling and applications in food and biomedical industries. Pectins from three Sugar beet (Beta vulgaris L.) commercial cultivars (Cadet, Coronado large, and SV MEI) were grown under irrigated desertic conditions and the influence of cultivar, on pectin yield, composition, physicochemical and gelling characteristics was investigated. The composition and chemical properties of pectin from the three cultivars were in general, statistically different, especially in the Syngenta Cadet cultivar, presenting higher protein (10.3%), neutral sugars (21.6%), galacturonic acid (55%) and ferulic acid (5.5 mg/g) content, which are important parameters for gel formation. All pectins gelled via oxidative coupling and the main ferulic acid dimers found were 8-5′ and 8-O-4′. Pectin from Cadet cultivar formed gels with higher hardness (6.65 N) and adhesiveness (12.2 N) values than the other two varieties. The results indicate that pectin composition is affected by the sugar beet cultivars reported herein, especially in ferulic acid content, which confer the covalent gelling capability. Sugar beet cultivars grown under desert conditions could be a source of gelling ferulated pectins for the food industry, as valuable as those obtained in temperate conditions.

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Section: Pectin Compositioncontrasting

confidence: 80%

Composition, Physicochemical Features, and Covalent Gelling Properties of Ferulated Pectin Extracted from Three Sugar Beet (Beta vulgaris L.) Cultivars Grown under Desertic Conditions

Lara-Espinoza¹,

Sánchez-Villegas²,

López‐Franco³

et al. 2020

Agronomy

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“…Precipitation by metal cations has been used for preparative and analytical purposes as a method for the rapid separation of macromolecules depending on their charge. For example, precipitation with copper(II) cations has been used for the purification of pectins, anionic plant cell wall polysaccharides [46][47][48][49]. This approach has been also used for determination of pectin content by photometry [50].…”

Section: Introductionmentioning

confidence: 99%

“…Metal precipitation avoids co-precipitation of other polysaccharides, which is observed in the ethanol precipitation [47]. Copper(II) cations selectively bind anionic regions of pectin chains, thereby separating pectic polysaccharides from oligosaccharides and proteins [48]. Consequently, counter cations have a certain affinity for negatively charged structural regions of macro-molecules, thereby selectively precipitating polyuronic acids, while neutral polysaccharides remain in solution.…”

Section: Introductionmentioning

confidence: 99%

Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy

et al. 2021

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Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical composition of three chosen dietary supplements (powder, tablets and capsules) containing hyaluronic acid was carried out using Fourier-transform infrared spectroscopy. Because of the low amount of analyte in all these samples, it was isolated or concentrated prior to the analysis using a suitable sequential fractionation protocol. Individual isolation procedures were established for each sample based on their declared composition. Firstly, the major components such as collagen or vitamins were removed to obtain polysaccharide fractions by the enzymatic treatment and/or washing out with the appropriate solvents. In some cases, the water insoluble part was removed from the rest dissolved in water. Then, hyaluronic acid was precipitated with copper(II) cations and thus separated from the other polysaccharides. Finally, the analyte was identified in the enriched fractions by the characteristic vibrational bands. The amount of hyaluronic acid in the purified fractions was determined in three ways: gravimetrically, spectrophotometrically, and using isotachophoresis. The combination of the appropriate preparative and analytical steps led to the successful evaluation of chemical composition, finding and quantification of hyaluronic acid in all the studied samples.

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“…Guo et al established the efficacy of purifying sugar beet pectin from non-pectin components by metal precipitation [19]. To assess the selectivity of copper ions in relation to binding pectin or non-pectin compounds, two fractions were fractionated from sugar beet pectin, namely copper-precipitated pectin and copper-unprecipitated pectin.…”

Section: Introductionmentioning

confidence: 99%

Beet pulp dietary fiber exposed to an extremely low-frequency electromagnetic field: detoxification properties

Tamova

Barashkina

Tretyakova

et al. 2021

Foods and Raw Materials

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Introduction. The lack of dietary fibre in the Russian people diet contributes to the development of various diseases. In this regard, it seems worthwhile to enrich foods with dietary fibre obtained from various types of raw materials. In our experiments, we used beet pulp. This study aimed to develop a technology for obtaining combined dietary fibre using the electrophysical method and evaluate its detoxification properties. Study objects and methods. Study objects were pectin substances and combined detoxicants from beet pulp obtained by extracting with succinic acid with and without an extremely low-frequency electromagnetic field (ELF EM) treatment. The profiles of combined detoxicants and pectin substances were identified by IR-Fourier spectrometry. Beet pectin, beet cellulose, and their combined detoxicants were tested for complexing (binding) capacity with respect to lead ions (Pb2+). For this, we applied the trilonometric method with some modifications. Results and discussion. The analysis of the absorption bands of carboxyl groups carbonyls revealed the presence of free carboxyl groups in the combined detoxicants. The combined detoxicant with a 1:0.5 ratio of cellulose and pectin substances showed a high complexing (binding) capacity (601 mg/Pb2+) with respect to lead ions (Pb2+). Conclusion. We developed a technology for producing combined detoxicants with a high complexing capacity with respect to lead ions analysed microstructures of gels obtained during the interaction between the combined detoxicant and lead acetate solution.

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Purifying sugar beet pectins from non-pectic components by means of metal precipitation

Cited by 31 publications

References 41 publications

Composition, Physicochemical Features, and Covalent Gelling Properties of Ferulated Pectin Extracted from Three Sugar Beet (Beta vulgaris L.) Cultivars Grown under Desertic Conditions

Composition, Physicochemical Features, and Covalent Gelling Properties of Ferulated Pectin Extracted from Three Sugar Beet (Beta vulgaris L.) Cultivars Grown under Desertic Conditions

Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy

Beet pulp dietary fiber exposed to an extremely low-frequency electromagnetic field: detoxification properties

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