Purified Recombinant A. fumigatus Allergens Induce Different Responses in Mice

Kurup, Viswanath P.; Xia, Jinqi; Crameri, Reto; Rickaby, D. A.; Choi, Hongyung; Flückiger, Sabine; Blaser, Kurt; Dawson, Chistopher A; Kelly, Kevin J.

doi:10.1006/clim.2000.4993

Cited by 54 publications

(67 citation statements)

References 35 publications

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“…Kurup et al informed that there was an enhanced Th2 response in the mice exposed to crude A. fumigatus extract and A. fumigatus spores [17]. In some other studies, it was observed that Th2-phenotype cytokines such as IL-4 and IL-10 increase susceptibility to A. fumigatus infection and contribute to the progression of infection [18,19].…”

Section: Discussionmentioning

confidence: 99%

Expression of the surface antigens of lymphocytes and the levels of cytokines in mice infected with Aspergillus fumigatus

Fidan

Kalkancı

Bolat

et al. 2008

J Infect Dev Ctries

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Background: Aspergillus fumigatus (A. fumigatus) is an opportunistic fungus that causes invasive aspergillosis. Determining the immune changes during A. fumigatus infection and the factors leading to such changes clearly will make it possible to prevent the spread of the infection and to provide new strategies in the treatment of infection. Thus, the present study aims at determining the changes of lymphocyte surface antigens which develop during A. fumigatus infection and the role of cytokines in immune response. Methodology: The expression of the surface antigens of lymphocytes was analysed by flow cytometry and the cytokine levels were determined by ELISA in a mouse model of aspergillosis. Results: It was observed that in mice infected by A. fumigatus the percentage of CD19+ B cells and the levels of IL-4 and IL-10 increased when compared to those in noninfected mice cells. Conclusions: These results suggest that Th2 type cytokines are important in the pathogenesis of A. fumigatus infection. Humoral immunity is considered to be effective during A. fumigatus infection because of the increase in Th2 type response.

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Section: Discussionmentioning

confidence: 99%

Expression of the surface antigens of lymphocytes and the levels of cytokines in mice infected with Aspergillus fumigatus

Fidan

Kalkancı

Bolat

et al. 2008

J Infect Dev Ctries

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show abstract

“…Earlier, the mice exposed to purified recombinant Af allergens Asp f 1, Asp f 3, and Asp f 4 exhibited inflammatory changes in the lungs and a predominantly Th2 immune response with elevated serum IgE and enhanced number of eosinophils in the peripheral blood and lungs (17). In the present study, the potential effect of low IgE binding Asp f 3 mutants on peripheral blood eosinophil levels was evidenced by 2 to 3 fold decrease in eosinophils in mice immunized with the mutants as compared to rAsp f 3 immunized mice.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, recombinant Asp f 3 and other purified Af allergens demonstrated enhanced IgE response and peripheral blood eosinophilia in a murine model of allergic aspergillosis (17). The lung histology showed infiltration of inflammatory cells, representing lymphocytes, macrophages, eosinophils and neutrophils.…”

Section: Introductionmentioning

confidence: 90%

“…The IgE reactivity of these proteins was studied by ELISA using sera from ABPA patients. (17,20). The first two injections were given intraperitoneally (i.p.)…”

Section: Asp F 3 and Its Deletion Mutantsmentioning

confidence: 99%

“…The IgE was presented as nanograms/ml of serum after converting the values using a standard curve obtained with purified mouse IgE. Antigen specific IgG 1 and IgG 2a were estimated by indirect ELISA (17). A serum dilution of 1:50 was used to determine specific IgG 1 and IgG 2a antibodies to Asp f 3 and the fragments.…”

Section: Antibody Estimations In Serummentioning

confidence: 99%

See 2 more Smart Citations

Immune response to n-terminal and c-terminal deletion mutants of Aspergillus fumigatus major allergen ASP F 3

Singh

Banerjee

Naik

et al. 2006

Indian J Clin Biochem

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Myeloid C-type lectin receptors in skin/mucoepithelial diseases and tumors

Tang

Makusheva

Sun

et al. 2019

Journal of Leukocyte Biology

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Myeloid C‐type lectin receptors (CLRs), which consist of an extracellular carbohydrate recognition domain and intracellular signal transducing motif such as the immunoreceptor tyrosine‐based activation motif (ITAM) or immunoreceptor tyrosine‐based inhibitory motif (ITIM), are innate immune receptors primarily expressed on myeloid lineage cells such as dendritic cells (DCs) and Mϕs. CLRs play important roles in host defense against infection by fungi and bacteria by recognizing specific carbohydrate components of these pathogens. However, these immune receptors also make important contributions to immune homeostasis of mucosa and skin in mammals by recognizing components of microbiota, as well as by recognizing self‐components such as alarmins from dead cells and noncanonical non‐carbohydrate ligands. CLR deficiency not only induces hypersensitivity to infection, but also causes dysregulation of muco‐cutaneous immune homeostasis, resulting in the development of allergy, inflammation, autoimmunity, and tumors. In this review, we introduce recent discoveries regarding the roles of myeloid CLRs in the immune system exposed to the environment, and discuss the roles of these lectin receptors in the development of colitis, asthma, psoriasis, atopic dermatitis, and cancer. Although some CLRs are suggested to be involved in the development of these diseases, the function of CLRs and their ligands still largely remain to be elucidated.

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Purified Recombinant A. fumigatus Allergens Induce Different Responses in Mice

Cited by 54 publications

References 35 publications

Expression of the surface antigens of lymphocytes and the levels of cytokines in mice infected with Aspergillus fumigatus

Expression of the surface antigens of lymphocytes and the levels of cytokines in mice infected with Aspergillus fumigatus

Immune response to n-terminal and c-terminal deletion mutants of Aspergillus fumigatus major allergen ASP F 3

Myeloid C-type lectin receptors in skin/mucoepithelial diseases and tumors

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