1969
DOI: 10.1128/jb.100.1.201-208.1969
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Purified Protoplasmic Peptides of Mycobacteria: In Vivo and In Vitro Comparison of the Species Specificity of Purified Protoplasmic Peptides and Purified Protein Derivatives of Mycobacterial Culture Filtrates

Abstract: The specificity of purified protein derivatives (PPD) prepared from the culture filtrates of Mycobacterium tuberculosis (PPD), M. kansasii (PPD-Y), M. intracellulare (PPD-B), and M. scrofulacewn (PPD-G) were compared to comparable protoplasmic extracts (PPP) of the same organisms by gel diffusion and delayed hypersensitivity reactions in sensitized guinea pigs. PPD and, to a lesser degree, PPD-Y demonstrated specificities sufficient to enable identification of homologously sensitized guinea pigs in the above g… Show more

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Cited by 13 publications
(8 citation statements)
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“…The observation by Stottmeier et al that the 4,000to 5,000-molecular-weight peptide that they isolated possessed significant species specificity (190) is noteworthy because the amino acid composition, although not the sequence, of this peptide was determined. It seems likely that the first identification of the structure of a my-…”
Section: Antigenic Specificitymentioning
confidence: 95%
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“…The observation by Stottmeier et al that the 4,000to 5,000-molecular-weight peptide that they isolated possessed significant species specificity (190) is noteworthy because the amino acid composition, although not the sequence, of this peptide was determined. It seems likely that the first identification of the structure of a my-…”
Section: Antigenic Specificitymentioning
confidence: 95%
“…It probably contained 3 to 4 residues of glucose, 12 residues each of aspartic and glutamic acid, and smaller amounts of six other amino acids (189). It proved to be active in the eliciting of delayed skin test reactions in sensitized animals and to have greater specificity in this regard than the corresponding PPD preparation from the same species of mycobacteria (190).…”
Section: Antigenic Peptidesmentioning
confidence: 98%
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“…Typical delayed hypersensitive skin reactions have been produced with bacillary extracts in sensitized animals (8,13,15). Protoplasmic preparations have been found to be as specific and sensitive as PPD for detection of delayed hypersensitivity (4, 12,14), and purified protoplasmic components have been claimed to be even more specific skin test antigens than PPD (24). Bacillary extracts also have been used serologically to differentiate and group mycobacterial species (9-11).…”
mentioning
confidence: 99%
“…In an earlier publication, we described the biological activity of tuberculin-active skin test antigens isolated from the protoplasm of mycobacteria (13). For this study we fractionated protoplasmic peptides of Mycobacterium tuberculosis strain H37Rv (PPP-TB) and subsequently investigated the chemical composition of the tuberculin-active fraction.…”
mentioning
confidence: 99%