Purified mulberry leaf lectin (MLL) induces apoptosis and cell cycle arrest in human breast cancer and colon cancer cells

Mundekkad, Deepa; Sureshkumar, Thavamani; Satheeshkumar, Padikara Kutty; Priya, Sulochana

doi:10.1016/j.cbi.2012.08.025

Cited by 68 publications

(28 citation statements)

References 36 publications

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“…Its dried leaves, called mulberry leaf in China, have been commonly used as a traditional Chinese medicine for its efficacies of hypoglycemic [1], hypolipidemic [2], antihypertensive [3], anti-atherosclerotic [4], anti-inflammatory [5], antioxidant [1,6,7], and antitumor [8]. Phytochemical studies have revealed that mulberry leaf contains various constituents, including flavonoids [9,10], alkaloids [11], polysaccharides [12], amino acids [13], nucleosides, microelements, and so on.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry

Zhang

Bai

Shu

et al. 2014

J of Separation Science

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An ultra high performance liquid chromatography coupled with a triple quadrupole mass detection and electrospray ionization mass spectrometry method has been established for the simultaneous determination of the 14 nucleosides and nucleobases, 24 amino acids and two main alkaloids in mulberry leaf. In this method, a complicated mobile phase, the flow rate of which was 0.4 mL/min, was applied to the gradient elution, which provided a satisfied separation of the 40 compounds. The present method was validated, and sufficient reproducibility and accuracy were obtained for the quantitative measurement of the 40 compounds. The method was subsequently applied to ten mulberry leaves and the results showed that almost all of these samples were rich in nucleosides, nucleobases, amino acids, and alkaloids. The proposed method, which is convenient and economical, could serve as a prerequisite for the quality control of mulberry leaf herbs and be applied analogously to other Chinese medicines.

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Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry

Zhang

Bai

Shu

et al. 2014

J of Separation Science

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“…In addition, to reduce cell proliferation, lectins may interact with receptors and other molecules present in cell surface and/or cytosol, activating cell death pathways. MCF-7 (human breast cancer) and HCT-15 (human colorectal adenocarcinoma) cells treated with a lectin isolated from Morus alba leaf showed morphological changes and DNA fragmentation that are characteristic of death by apoptosis, which was confirmed by the authors using staining with annexin V and acridine orange/ethidium bromide; in addition, an increased activity of caspase 3 was detected [182]. On the other hand, the Bauhinia forficata seed lectin induced necrosis and secondary necrosis in MCF-7 cells, with caspase 9 inhibition [21].…”

Section: Lectins As Anticancer Agentsmentioning

confidence: 87%

Lectins, Interconnecting Proteins with Biotechnological/Pharmacological and Therapeutic Applications

Coelho

Silva

Lima

et al. 2017

Evidence-Based Complementary and Alternative Medicine

148

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Lectins are proteins extensively used in biomedical applications with property to recognize carbohydrates through carbohydrate-binding sites, which identify glycans attached to cell surfaces, glycoconjugates, or free sugars, detecting abnormal cells and biomarkers related to diseases. These lectin abilities promoted interesting results in experimental treatments of immunological diseases, wounds, and cancer. Lectins obtained from virus, microorganisms, algae, animals, and plants were reported as modulators and tool markers in vivo and in vitro; these molecules also play a role in the induction of mitosis and immune responses, contributing for resolution of infections and inflammations. Lectins revealed healing effect through induction of reepithelialization and cicatrization of wounds. Some lectins have been efficient agents against virus, fungi, bacteria, and helminths at low concentrations. Lectin-mediated bioadhesion has been an interesting characteristic for development of drug delivery systems. Lectin histochemistry and lectin-based biosensors are useful to detect transformed tissues and biomarkers related to disease occurrence; antitumor lectins reported are promising for cancer therapy. Here, we address lectins from distinct sources with some biological effect and biotechnological potential in the diagnosis and therapeutic of diseases, highlighting many advances in this growing field.

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“…The translocation of PS to the outside or exposed side of the membrane is an early event in the apoptotic process; therefore, Annexin V staining is considered a marker for early stage apoptosis. 38 Mitochondria are essential organel for both vital (energy production) and lethal (apoptosis) functions in physiological and pathological scenarios and decreased mitochondrial membrane potential could caused to apoptotic cell death in cells. 39 Lee et al demonstrated that morusin which is isolated from root bark of Morus australis and inhibits growth of human colon cancer cells via activation of caspases and inhibition of NF-κB pathway, 40 while Faria et al reported that blueberry antho cyanin-pyruvic acid adduct extract exhibits cytotoxic effect on human breast cancer cells via caspase activation.…”

Section: Discussionmentioning

confidence: 99%

Morus rubra Extract Induces G1 Cell Cycle Arrest and Apoptosis in Human Lung and Prostate Cancer Cells

Turan¹,

Demir²,

Kilinç³

et al. 2017

IJPER

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Objective: Cancer is one of the most deadly types of disease and evasion from apoptosis and unstoppable cell proliferation are accepted as its distinctive features. Many studies have evaluated the cytotoxic effect of different Morus species but, there is no study about cytotoxic effect of Morus rubra. In this study we aimed to evaluate phenolic composition, antioxidant properties and cytotoxic effect of acidified dimethyl sulfoxide extract of M. rubra (AMRE). Method: Antioxidant properties, phenolic composition and cytotoxic effect of AMRE were determined using spectrophotometric methods, HPLC, and MTT assay, respectively. Then, mechanisms of cytotoxic effect of AMRE on human prostate (PC-3) and lung (A549) cancer cells were examined in regard to cell cycle, apoptosis and mitochondrial membrane potential using flow cytometric methods. Results: Total phenolic content and reducing power values were 11.9 mg gallic acid equivalents and 42.9 mg trolox equivalents per g sample, respectively. Ascorbic and gallic acid were detected in AMRE as major antioxidant compounds. We determined that AMRE increased cell cycle arrest at G 1 phase and exhibited apoptotic features via decreasing mitochondrial membrane potential in both prostate and lung cancer cells. Conclusion: These findings demonstrate that M. rubra extract can affect the behavior of human prostate and lung cancer cells in vitro conditions, and this effect now needs to be investigated in vivo.

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Purified mulberry leaf lectin (MLL) induces apoptosis and cell cycle arrest in human breast cancer and colon cancer cells

Cited by 68 publications

References 36 publications

Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry

Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry

Lectins, Interconnecting Proteins with Biotechnological/Pharmacological and Therapeutic Applications

Morus rubra Extract Induces G1 Cell Cycle Arrest and Apoptosis in Human Lung and Prostate Cancer Cells

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