PURIFIED DROSOPHILA Α-Amylase ISOZYMES: GENETICAL, BIOCHEMICAL, AND MOLECULAR CHARACTERIZATION

Doane, Winifred W.; Abraham, Irene; Kolar, Miriam; Martenson, Russell E.; Deibler, Gladys E.

doi:10.1016/b978-0-12-472704-5.50041-0

Cited by 34 publications

(17 citation statements)

References 29 publications

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“…Within the melanogaster complex the only exception is D. gests such a possibility. Also, a male specific enzyme under independent genetic control and suggestive of a duplication has been identified in D. hydei (Doane et a!., 1975). Further studies in other species of Drosophila are thus needed.…”

Section: Introductionmentioning

confidence: 99%

Amylase gene duplication: an ancestral trait in the Drosophila melanogaster species subgroup

et al. 1987

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Electrophoretic polymorphism of amylases was studied in 45 geographic populations of the two cosmopolitan sibling species, D. melanogaster and D. simulans, and in one to three populations or strains of six other species in the D. melanogaster subgroup. Two species, D. erecta and D. orena, for which only a few strains were available were monomorphic. In the other species 2 or 3 amylase variants were identified while in D. melanogaster, 12 electrophoretic variants were characterized. Altogether 17 different amylase isozymes have been observed. The contrast in the level of polymorphism between D. melanogaster and the other species cannot be explained simply by the occurrence of a duplication in the former species. Genetic analysis demonstrated the existence of a duplication in at least 4 other species, namely D. simulans, D. mauritiana, D. yakuba, and D. teissieri. It is therefore suggested that the duplication occurred in a common ancestor and the phylogenetic implications of these observations are discussed.

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Section: Introductionmentioning

confidence: 99%

Amylase gene duplication: an ancestral trait in the Drosophila melanogaster species subgroup

et al. 1987

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“…This enzyme system is very suitable for this kind of study, because in addition to the interests mentioned above, biochemical handling of the enzyme is very easy, because of its high stability and its activity being present in flies in large amounts. Moreover, proceduers of determining activity and purification are well-established (Doane et al 1975 ;Silvanovich and Hill 1976). We have chosen D, virilis as the material for investigation for the following reasons.…”

Section: Introductionmentioning

confidence: 99%

Genetic regulation of amylase activity in Drosophila virilis. I. Activity variation among laboratory strains.

Tejima

Ohba

1981

The Japanese journal of genetics

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Extensive variation of amylase activity in adults and 3rd instar larvae was found among D. virilis strains homozygous for both Amy' allele and Amys allele. The activity in adult is not correlated with that in larva, suggesting that the amylase activity is differently regulated between the two stages, although the same amylase locus is expressed. Cross experiments between the strain with the highest activity and the strain with the lowest activity reveal that amylase activity in adult is an additive character and that amylase activity co-segregates with amylase genotype. Electrophoretic zymogram of the F1 progeny and the mixture of the parents suggested that the genetic factors for amylase activity are probably not identical to the Amy and that they are likely to operate on the activity determination in a trans-active manner.

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“…For the Mdh and a-Amy loci, we found that the frequencies of alternative alleles were also rather low. As the a-amylase enzyme is encoded by a duplicated locus we did not calculate allele frequencies, thus only the phenotype frequencies are presented in the tables (Doane et al, 1975;Singh et al, 1982 (Batterham et al, 1984). In some of the collecting sites, however, we found 2 different rare alleles at this locus.…”

Section: Introductionmentioning

confidence: 97%

“…The a-Gpdh locus was actually monomorphic with rare alleles appearing mainly in region II. Similarly to the Adh, the a Amy locus had 2 rare alleles (Doane et al, 1975) that were mainly found in populations of region 111.…”

Section: Introductionmentioning

confidence: 99%

“…It would also be interesting, however, to study the difference in the genetic diversity of the 2 kinds of populations. In the case of laboratory populations, several observations have revealed differences in the average frequency of heterozygotes when Drosophilids were kept in homogeneous and heterogeneous environments (Powell, 1971;McDonald and Ayala, 1974;Hale and Birley, 1983 (O'Brien, 1973;Doane et al, 1975;Clark, 1983;Winberg ef al., 1983;Batterham et al, 1984 pectively. At the Adh locus, almost all the populations were polymorphic; however, the frequency of the slow allele was rather low.…”

Section: Introductionmentioning

confidence: 99%

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A comparison of the level of enzyme polymorphism in cosmopolitan Drosophila species between populations collected in distilleries and in their surroundings in Hungary

Pecsenye¹

1989

Genet. Sel. Evol.

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PURIFIED DROSOPHILA Α-Amylase ISOZYMES: GENETICAL, BIOCHEMICAL, AND MOLECULAR CHARACTERIZATION

Cited by 34 publications

References 29 publications

Amylase gene duplication: an ancestral trait in the Drosophila melanogaster species subgroup

Amylase gene duplication: an ancestral trait in the Drosophila melanogaster species subgroup

Genetic regulation of amylase activity in Drosophila virilis. I. Activity variation among laboratory strains.

A comparison of the level of enzyme polymorphism in cosmopolitan Drosophila species between populations collected in distilleries and in their surroundings in Hungary

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