Purification, structural features and immunostimulatory activity of novel polysaccharides from Caulerpa lentillifera

Sun, Yujiao; Gong, Guiping; Guo, Yiming; Wang, Zhongfu; Song, Shuang; Zhu, Beiwei; Zhao, Lili; Jiang, Jinju

doi:10.1016/j.ijbiomac.2017.12.016

Cited by 71 publications

(52 citation statements)

References 48 publications

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“…The broad absorption bands with strong intensities at approximately 1420 and 1374 cm −1 in the spectra of PEP1‐A, PEP2‐A and PEP3‐A were likely due to deforming vibrations of the C–H bond (Zeng et al , ,b). Absorption peaks at 1240 and 816 cm −1 represent the sulphuryl groups (

{SO}_{3}^{-}

) in PEP1‐A, PEP2‐A and PEP3‐A (Sun et al , ,b), and may include the asymmetrical S=O stretching vibration and a symmetrical C–O–S stretching vibration associated with a C–O–SO 3 group, respectively (Sun et al , ,b). The peak at 1076.04 cm −1 was attributed to a C–O–C stretching vibration peak.…”

Section: Resultsmentioning

confidence: 99%

“…According to the molecular weight measurement, the highest molecular weight was observed for PEP2‐A at 9.506 × 10 6 Da. Polysaccharides with different molecular weights (Mw) have different levels of viscosity, water solubility and biological activity, such as the ability to pass through barriers and enter the cell (Sun et al , ,b). Regarding the monosaccharide composition, PEP1‐A and PEP2‐A were composed of four monosaccharides: fucose, galactose, glucose and mannose.…”

Section: Discussionmentioning

confidence: 99%

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Extraction, isolation, characterisation, antioxidant and anti‐fatigue activities of Pleurotus eryngii polysaccharides

Zhao¹,

Chen²,

Jia³

et al. 2020

Int J of Food Sci Tech

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Pleurotus eryngii polysaccharide (PEP) was obtained using hot water extraction and ethanol precipitation. Three purified polysaccharide fractions (namely PEP1-A, PEP2-A and PEP3-A) were obtained from PEP using DEAE-cellulose-52 chromatography and a gel permeation Sephadex G-100 column. Firstly, this paper examined the characterisation of PEP1-A, PEP2-A and PEP3-A. The corresponding molecular weights were 5.378 9 10 5 , 9.506 9 10 6 and 4.975 9 10 5 Da, respectively. PEP1-A, PEP2-A and PEP3-A had similar monosaccharide compositions. PEP1-A was b-configuration, and PEP2-A and PEP3-A were a-configuration. PEP1-A, PEP2-A and PEP3-A had pyran-type rings, (1 ? 3) glucose and (1 ? 6) galactose linkages. Secondly, PEP, PEP1-A, PEP2-A and PEP3-A possessed antioxidant activities, and PEP was best. Therefore, only PEP was used to study its anti-fatigue activity in vivo. The result proved that PEP had anti-fatigue activity. PEP could be used as a valuable natural food supplement for preventing anti-fatigue or functional food.

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{SO}_{3}^{-}

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Extraction, isolation, characterisation, antioxidant and anti‐fatigue activities of Pleurotus eryngii polysaccharides

Zhao¹,

Chen²,

Jia³

et al. 2020

Int J of Food Sci Tech

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“…In the three spectra, the absorption peaks at approximately 1610-1655 cm −1 corresponded to carbonyl groups (C=O) and were caused by the bending mode of bound water, while the absorption peak at approximately 1400 cm −1 was the C-O stretching vibration [26,29,34,35]. A strong absorption peak at 1615 cm −1 presented carboxyl groups (COO−) in GUPS-III, indicating the existence of uronic acids [36,37]. A strong band between 950 and 1160 cm −1 in the three spectra of GUPS were attributed to the stretching vibrations of the pyranose ring [38].…”

Section: The Analysis Of Gups By Ft-ir Spectroscopymentioning

confidence: 99%

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities. Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively. The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different. The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages. Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I. An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs. These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.

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“…Recently, a xylogalactomannan fraction containing galactose, mannose and xylose in molar ratio 2.4:2.2:1, and 21% sulfate was isolated from C. lentillifera . It consisted in 3-linked and terminal β- d -galactopyranose units, the former partially sulfated on C-6, 4-, 2,4-, and 2-linked β- d -mannopyranose units, the latter sulfated on C-3, and 4-linked 3-sulfated, and terminal β- d -xylopyranose units ( Sun et al, 2018 ; Sun et al, 2020 ). This sample was reported to have a molecular weight of 3877.8 kDa, which is quite high for a sulfated polysaccharide, leading us to speculate that it could comprise several aggregated polysaccharides.…”

Section: Sulfated Polysaccharides From Coenocytic Green Algaementioning

confidence: 99%

Diversity of Sulfated Polysaccharides From Cell Walls of Coenocytic Green Algae and Their Structural Relationships in View of Green Algal Evolution

2020

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Seaweeds biosynthesize sulfated polysaccharides as key components of their cell walls. These polysaccharides are potentially interesting as biologically active compounds. Green macroalgae of the class Ulvophyceae comprise sulfated polysaccharides with great structural differences regarding the monosaccharide constituents, linearity of their backbones, and presence of other acidic substituents in their structure, including uronic acid residues and pyruvic acid. These structures have been thoroughly studied in the Ulvales and Ulotrichales, but only more recently have they been investigated with some detail in ulvophytes with giant multinucleate (coenocytic) cells, including the siphonous Bryopsidales and Dasycladales, and the siphonocladous Cladophorales. An early classification of these structurally heterogeneous polysaccharides was based on the presence of uronic acid residues in these molecules. In agreement with this classification based on chemical structures, sulfated polysaccharides of the orders Bryopsidales and Cladophorales fall in the same group, in which this acidic component is absent, or only present in very low quantities. The cell walls of Dasycladales have been less studied, and it remains unclear if they comprise sulfated polysaccharides of both types. Although in the Bryopsidales and Cladophorales the most important sulfated polysaccharides are arabinans and galactans (or arabinogalactans), their major structures are very different. The Bryopsidales produce sulfated pyruvylated 3-linked β- d -galactans, in most cases, with ramifications on C6. For some species, linear sulfated pyranosic β- l -arabinans have been described. In the Cladophorales, also sulfated pyranosic β- l -arabinans have been found, but 4-linked and highly substituted with side chains. These differences are consistent with recent molecular phylogenetic analyses, which indicate that the Bryopsidales and Cladophorales are distantly related. In addition, some of the Bryopsidales also biosynthesize other sulfated polysaccharides, i.e., sulfated mannans and sulfated rhamnans. The presence of sulfate groups as a distinctive characteristic of these biopolymers has been related to their adaptation to the marine environment. However, it has been shown that some freshwater algae from the Cladophorales also produce sulfated polysaccharides. In this review, structures of sulfated polysaccharides from bryopsidalean, dasycladalean, and cladophoralean green algae studied until now are described and analyzed based on current phylogenetic understanding, with the aim of unveiling the important knowledge gaps that still exist.

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Purification, structural features and immunostimulatory activity of novel polysaccharides from Caulerpa lentillifera

Cited by 71 publications

References 48 publications

Extraction, isolation, characterisation, antioxidant and anti‐fatigue activities of Pleurotus eryngii polysaccharides

Extraction, isolation, characterisation, antioxidant and anti‐fatigue activities of Pleurotus eryngii polysaccharides

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

Diversity of Sulfated Polysaccharides From Cell Walls of Coenocytic Green Algae and Their Structural Relationships in View of Green Algal Evolution

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