Purification, size, and morphology of a mosquito-borne animal virus, Semliki Forest virus

Cheng, Ping-Yao

doi:10.1016/0042-6822(61)90139-8

Cited by 42 publications

(8 citation statements)

References 11 publications

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“…Treatment of another small sample of viral RNA with ribonuclease followed Sucrose density gradient analysis of 'Hlabeled RNA from Semliki Forest virus (SFV)-infected chick embryo fibroblast cells. In (A), a 50-1tliter sample of 3H-labeled SFV RNA was layered directly on a 4.4-ml, 6 to 30% linear sucrose gradient prepared in 0.1 M NaCl, 0.01 mI tris(hydroxymethyl)aminomethane (pH 7.2), and 0.001 M ethylenediaminetetraacetic acid; in (B) another sample (50 ,uliters) was treated with ribonuclease (0.1 ,ug/ml) for 30 min at 37 C and then applied to a separate gradient. Under these conditions viral RNA is hydrolyzed to the same extent with ribonuclease concentrations of 0.1 ,ug/ml or 1 ,ug/ml (reference 10; R. M. Friedman, unpublished observation).…”

Section: Resultsmentioning

confidence: 99%

Analysis of Arbovirus Ribonucleic Acid Forms by Polyacrylamide Gel Electrophoresis

Levin

Friedman

1971

J Virol

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MATERIALS AND METHODS Materials. Actinomycin D was a gift from Merck, Sharp, and Dohme Research Laboratories (Div. of Merck & Co., Inc., Rahway, N. J.). Adenosine-2, 8-3H (7.0 Ci/mmole) and uridine-5-3H (19.9 Ci/mmole) were purchased from New England Nuclear Corp. (Boston, Mass.); 32P-phosphoric acid (carrier free) was obtained from Tracerlab (Waltham, Mass.); and 3H-guanosine triphosphate (1.0 Ci/ mmole) was purchased from Schwarz BioResearch Inc. (Orangeburg, N. Y.). Adenosine triphosphate, uridine triphosphate, and cytidine triphosphate were purchased from General Biochemicals, Inc. (Chagrin Falls, Ohio). Pancreatic ribonuclease, Pronase, phosphoenolpyruvate, pyruvate kinase, and dithiothreitol were obtained from Calbiochem (Los Angeles, Calif.). N, N, N', N' tetramethylethylenediamine, acrylamide, and N, N'-methylene bisacrylamide were purchased from Eastman Organic Chemicals (Rochester, N. Y.). The acrylamide and bisacrylamide were recrystallized according to Loening (14). Agarose (Seakem) was obtained from Bausch and Lomb Inc. (Rochester, N. Y.). Ribonuclease-free sucrose was purchased from Mann Research Laboratories (Orangeburg, N. Y.). Triton X-100 was obtained from Packard Instrument Co., Inc. (Downers Grove, Ill.) and toluene-Liquifluor was obtained from New England Nuclear Corp. Cells and virus pools. The procedures for prepara-504

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Section: Resultsmentioning

confidence: 99%

Analysis of Arbovirus Ribonucleic Acid Forms by Polyacrylamide Gel Electrophoresis

Levin

Friedman

1971

J Virol

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“…After alphavirus entry and uneoating, which may take place in the close vicinity of endosomes, the genome RNA is subjected to translation. As a single virus particle can cause infection (155), we have to think that one RNA molecule is first translated, followed by its recruitment as a template for minus-strand synthesis. These primary events cannot be detected by available techniques.…”

Section: The Replication Complexmentioning

confidence: 99%

Functions of alphavirus nonstructural proteins in RNA replication

Kääriäinen

Ahola

2002

Progress in Nucleic Acid Research and Molecular Biology

107

111

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“…Thermal decay of L cell-propagated CTF virus appears to proceed at a rate similar to that characteristic of other arboviruses which have been studied (1,2,3,12,14,17). Koprowski and Cox (11) previously reported that mouse-brain CTF virus was completely inactivated during exposure to a temperature of 60 C for 30 min; however, the virus was relatively stable at temperatures of 37 C for several days while in a serum-saline mixture.…”

Section: Discussionmentioning

confidence: 75%

Colorado Tick Fever Virus in Cell Culture II. Physical and Chemical Properties

Trent

Scott

1966

J Bacteriol

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Heat-inactivation kinetics for Colorado tick fever (CTF) virus grown in L cells indicated that more than one rate constant was involved for inactivation at each exposure temperature. An Arrhenius plot of the data indicated the inactivation rate constants to be dependent on the absolute temperature. The energy of activation, for thermal inactivation of the virus, was 17,289 calories per mole, with the Qlo being 2.6. The optimal pH range for maintenance of CTF viral infectivity was determined to be 7.5 to 7.8. The infectivity of CfF virus was stable to freezing and thawing in diluents which contained: 50% calf serum, 20% glucose, 20% glycerol, 10% bovine serum albumin, 20 mm glutamine, and 2% gelatin. CTF virus replication was insensitive to inhibition by 5-fluoro-2'-deoxyuridine and 5-bromo-2'-deoxyuridine, whereas herpes simplex virus was markedly inhibited, as reported by others. Actinomycin D inhibited CIF virus replication when cells were pretreated for 24 and 12 hr prior to infection, but not when the inhibitor was added at the time of infection. The nucleic acid of CITF virus appears to be of the ribose type.

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Purification, size, and morphology of a mosquito-borne animal virus, Semliki Forest virus

Cited by 42 publications

References 11 publications

Analysis of Arbovirus Ribonucleic Acid Forms by Polyacrylamide Gel Electrophoresis

Analysis of Arbovirus Ribonucleic Acid Forms by Polyacrylamide Gel Electrophoresis

Functions of alphavirus nonstructural proteins in RNA replication

Colorado Tick Fever Virus in Cell Culture II. Physical and Chemical Properties

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