Purification, propriétés et spécificité d'une hétéroglycosidase des graines de sarrasin

Syntheses of R-β-rutinosides by rutin-degrading reaction

“…6)-a/b-D-glucopyranosyl) extensively exists in many plants in a pattern of glycosides, of which most are flavonoid glycosides [1], but few were found in free form. Up to now, all the chemical syntheses of rutinose from flavonoid started from natural rutinoside with acid-or enzyme-catalyzed hydrolysis reaction [2][3][4][5][6][7][8][9][10][11][12].…”

“…Some studies revealed that there were flavonol-3-heterodisaccharide glycosidase [11] and rutin-degrading enzyme (RDE) [8,10,13,14] in Fagopyrum plants, which can decompose rutin into quercetin and rutinose in water solution [13 -15], and have higher selectivity of substrates. Our previous research also showed that RDE in the seed of Fagopyrum tataricum (L.) Gaertn had not only very high activity, but also strong resistance against acid, base, heat, as well as high concentrated ethanol [16].…”