Purification, physicochemical characterization, and immunohistochemical localization of a major 11.7 S glycoprotein from the jelly coats of the anuran Lepidobatrachus laevis

Carroll, Edward J.; Wei, Susan H.; Nagel, Glenn M.

doi:10.1016/0003-9861(91)90306-4

Cited by 10 publications

(14 citation statements)

References 21 publications

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“…Biochemical, compositional studies of amphibian jelly macromolecules are few in number (9,18,28,35,43) and have mainly been accomplished on anuran species. Such studies have utilized soluble jelly preparations produced using alkaline mercaptan reduction of disulfide bonds required for maintenance of the jelly solid state (1 7).…”

Section: Discussionmentioning

confidence: 99%

“…Typically, each of the individual envelopes of Bufo boreas, Rana pipiens (J. L. Hedrick, personal communication) and Xenopus laevis (43) and, more recently, Lepidobatrachus laevis (9,10) are brought into solution using alkaline mercaptans. A. mexicanum embryos were suspended in 0.15 M 2-mercaptoethanol at pH 10.5 and room temperature.…”

Section: Observations On Jelly Coat Structure and Solubiiizationmentioning

confidence: 99%

“…The 110 kD A. mexicanurn polypeptide was selected for further study because it was abundant and easily isolated. Only one other amphibian jelly coat polypeptide has been isolated to homogeneity and physicochemically characterized (9), therefore extensive comparisons are not possible. In the later study, the 29.7 kD glycoprotein was reported to have a high fraction of acidic amino acids (Glx+Asx=32.4 mole%) and a low fraction of basic amino acids (Arg+Lys= 12.2%).…”

Section: Glu-gln-tyr-asn-tyrb-glu-pro-val-phe-serlo-glnmentioning

confidence: 99%

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Structure and Macromolecular Composition of the Jelly Coats of the Urodele Ambystoma mexicanum

1992

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Cleavage-stage embryos of the neotenic urodele Ambystoma mexicanum are surrounded by a fertilization envelope and four macroscopic jelly coats termed J, (innermost) through J4 (outermost). In sections prepared for light microscopy, each of the jelly layers stained with protein stains and the periodic acid-Schiff's reagent, but only J1 stained with alcian blue at pH 2.5. These results suggest that each layer consists of proteins and glycoproteins and that J, uniquely contains some sulfate esters. Only J4 was solubilized with alkaline mercaptan treatment in situ, however, the isolated inner jelly complex (J1, J2 and J3) was easily dissolved in this reagent suggesting that solvent access is impaired in situ. A single alcian blue-staining component plus one protein-staining component were detected on reducing polyacrylamide gel electrophoresis of outer jelly (J4). In the inner jelly complex (J1, J2, J3), two protein-staining components were detected and no alcian blue-staining components were observed. A predominant polypeptide of 11 0,000 molecular weight was detected and purified to homogeneity on reducing and denaturing gels of the inner jelly complex. Amino acid analysis of the polypeptide demonstrated a slightly higher fraction of acidic over basic amino acids (GIx+Asx=18.1 mole% vs. Arg+Lys=11.7 mole%). The N-terminal amino acid was Glu and the sequence of the first eleven amino acids was determined.

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Section: Discussionmentioning

confidence: 99%

Section: Observations On Jelly Coat Structure and Solubiiizationmentioning

confidence: 99%

Section: Glu-gln-tyr-asn-tyrb-glu-pro-val-phe-serlo-glnmentioning

confidence: 99%

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Structure and Macromolecular Composition of the Jelly Coats of the Urodele Ambystoma mexicanum

1992

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“…The solution was stored at -20°C. Hatching medium was collected after 175 chemically dejellied embryos (3) hatched from their FEs in approximately 250 mL of aged tap water. The hatching medium was lyophilized and 25 mg of the dry powder was resuspended in 0.05 Tris-modified Ringer's solution (contains 10 mM Tris-HCI, pH 7.4).…”

Section: Collection Of Activated Egg Exudate and Crude Hatching Enzymementioning

confidence: 99%

“…Lepidobatrachus has only two jelly layers with a relatively simple molecular composition and one component has been purified (3,4). In the present work, the structural, chemical and macromolecular composition of the Lepidobatrachus primary egg envelopes are characterized, and mechanisms for these envelope conversions are examined.…”

Section: Introductionmentioning

confidence: 99%

The Primary Egg Envelope of the Anuran Lepidobatrachus laevis: Physicochemical and Macromolecular Alterations During Development

Peavy¹,

Carroll²

1993

Dev Growth Differ

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The coelomic egg envelope (CE) of the frog Lepidobatrachus laevis has a network of fibrillar bundles which disperse after transit through the oviduct. Following oviposition, the egg vitelline envelope (VE) has an additional amorphous zone on the exterior surface. The fertilization envelope (FE) formed after fertilization, appears to be very similar to the VE. The CEs, VEs, FEs and hatched envelopes (FE,,) were manually isolated. The CE, VE and FE were solubilized at 100°C using denaturing conditions, but were only partially solubilized in phosphate buffer, pH 7.0. All envelopes and several purified polypeptides from the VE and FE were analyzed using gel electrophoresis and one-dimensional peptide mapping. Each of the envelopes contained 9 major polypeptides ranging from 11 8.5 to 22 kD and 8-1 2 minor polypeptides. Several envelope components were added/removed in the conversions based on the results of experiments in which preparations were incubated with activated egg exudate and crude hatching enzyme; some of these transformations were mimicked by tryptic and chymotryptic digestions. Therefore, serine proteases may be involved in envelope processing in vivo. Lepidobatrachus CE polypeptides and several major components from the VE, FE and FEh were crossreactive with antibodies against Xenopus VE*.

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Artificial fertilization for amphibian conservation: Current knowledge and future considerations

Kouba¹,

Vance

Willis³

2009

Theriogenology

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Purification, physicochemical characterization, and immunohistochemical localization of a major 11.7 S glycoprotein from the jelly coats of the anuran Lepidobatrachus laevis

Cited by 10 publications

References 21 publications

Structure and Macromolecular Composition of the Jelly Coats of the Urodele Ambystoma mexicanum

Structure and Macromolecular Composition of the Jelly Coats of the Urodele Ambystoma mexicanum

The Primary Egg Envelope of the Anuran Lepidobatrachus laevis: Physicochemical and Macromolecular Alterations During Development

Artificial fertilization for amphibian conservation: Current knowledge and future considerations

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