Purification, physicochemical and thermodynamic studies of antifungal chitinase with production of bioactive chitosan-oligosaccharide from newly isolated Aspergillus griseoaurantiacus KX010988

Shehata, Abeer N.; Aty, Abeer A. Abd El; Darwish, Doaa A.; Wahab, Walaa A. Abdel; Mostafa, Faten A.

doi:10.1016/j.ijbiomac.2017.09.071

Cited by 46 publications

(26 citation statements)

References 38 publications

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“…Most bacterial chitinases have been prepared from Serratia , Chromobacterium , Klebsiella , Bacillus , and Streptomyce s genus . Screening bacteria with high chitinase activity to convert chitin into chitooligosaccharide might have great potential to improve chitinolytic enzymes for food industrial and medical applications .…”

Section: Introductionmentioning

confidence: 99%

Characterization of a chitinase from Salinivibrio sp. BAO‐1801 as an antifungal activity and a biocatalyst for producing chitobiose

2018

J Basic Microbiol

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Salinivibrio genus is commonly found in salted seafood products. In this study, chitinase produced by Salinivibrio sp. BAO-1801 isolated from salted fermented shrimp was purified and subsequently characterized. The molecular weight of BAO-1801 chitinase was approximately 94.2 kDa by SDS-PAGE analysis. It was classified as a chitinase C based on homology analysis of its N-terminal amino acid residues. This strain BAO-1801 chitinase was then used for synthesis of (GlcNAc)2. Degradation of colloidal chitin and N-acetyl chitooligosaccharides by BAO-1801 chitinase was then analyzed and (GlcNAc)2 was identified as the main product by thin layer chromatography and high-performance liquid chromatography. Effects of temperature and pH on activity and stability of BAO-1801 chitinase were also investigated. Furthermore, this enzyme inhibited fungal growth in a dose-dependent manner. Taken together, these results suggest that this Salinivibrio or its chitinase can be used for the enzymatic degradation of chitin to produce chitobiose in industrial process.

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Section: Introductionmentioning

confidence: 99%

Characterization of a chitinase from Salinivibrio sp. BAO‐1801 as an antifungal activity and a biocatalyst for producing chitobiose

2018

J Basic Microbiol

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“…Studies have found that a number of metabolites from microbial strains including lipopeptide, macrolide, surfactin (Santos et al 2018), fengycin (Fan et al 2017), iturin , bacillomycin D (Tabbene et al 2016), chitinase (Shehata et al 2018), and other active proteins (Li et al 2009) have antimicrobial activity. It is reported that the mainly antimicrobial substances produced by Bacillus are presented with different proteins and lipopeptides (Abriouel et al 2011).…”

Section: Discussionmentioning

confidence: 99%

A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities

et al. 2020

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Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify active ingredient from Bacillus amyloliquefaciens GN59 and study its antibacterial activity. A novel compound was isolated from GN59 by column chromatography on silica gel and HPLC purification. The chemical structure was identified as α-dglucopyranosyl-(1 → 1′)-3′-amino-3′-deoxy-β-d-glucopyranoside (a,β-3-trehalosamine) on the basis of spectroscopic analysis. This is the first report about a,β-3-trehalosamine isolated from biological resources on an antibiotic activity against pathogenic bacterium. The 3′-neotrehalosamine displayed antibacterial activity across a broad spectrum of microorganisms, including different gram-positive and gram-negative bacteria, with minimal inhibitory concentration (MIC) values ranging from 0.5 to 0.7 mg/mL. The results indicated that the 3′-neotrehalosamine from GN59 might be a potential candidate for bactericide.

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“…For example, the chitinase produced by A. niger LOCK 62 inhibited the growth of Fusarium culmorum, Fusarium solani, and Rhizoctonia solani, but not the growth of Botrytis cinerea, Alternaria alternata, and Fusarium oxysporum (Swiontek Brzezinska and Jankiweicz 2012). The chitinase isolated from A. griseoaurantiacus KX010988 inhibited the growth of F. solani (Shehataa et al 2018).…”

Section: Antifungal Activitymentioning

confidence: 99%

Production and characterization of a thermostable antifungal chitinase secreted by the filamentous fungus Aspergillus niveus under submerged fermentation

et al. 2018

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The filamentous fungus produced extracellular antifungal chitinase when cultured under submerged fermentation (SbmF) using crab shells as the carbon source. Maximal chitinase production was achieved at 192 h of cultivation using minimal medium containing 1% chitin. The enzyme was purified 1.97-fold with 40% recovery by ammonium sulfate precipitation and Sephadex G-100 gel filtration. The molecular mass was estimated to be 44 kDa by both 12% SDS-PAGE and Sepharose CL-6B gel filtration. Maximal chitinase activity was obtained at 65 °C and pH 5.0. The enzyme was fully stable at 60 °C for up to 120 min and the enzymatic activity was increased by Mn. In the presence of reducing and denaturing compounds, the enzyme activity was not drastically affected. The chitinase was able to hydrolyze colloidal chitin, azure chitin, and 4-nitrophenyl -acetyl-β-D glucosaminide; for the latter, the and maximal velocity () were 3.51 mM and 9.68 U/mg of protein, respectively. The chitinase presented antifungal activity against (MIC = 84 µg/mL), (MIC = 21 µg/mL), (MIC = 24 µg/mL), (MIC = 24 µg/mL), and (MIC = 21 µg/mL). The fungus was able to produce a thermostable and denaturation-resistant chitinase able to inhibit fungal development, signaling its biotechnological potential.

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Purification, physicochemical and thermodynamic studies of antifungal chitinase with production of bioactive chitosan-oligosaccharide from newly isolated Aspergillus griseoaurantiacus KX010988

Cited by 46 publications

References 38 publications

Characterization of a chitinase from Salinivibrio sp. BAO‐1801 as an antifungal activity and a biocatalyst for producing chitobiose

Characterization of a chitinase from Salinivibrio sp. BAO‐1801 as an antifungal activity and a biocatalyst for producing chitobiose

A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities

Production and characterization of a thermostable antifungal chitinase secreted by the filamentous fungus Aspergillus niveus under submerged fermentation

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