Purification of β -mannanase derived from Bacillus subtilis ATCC 11774 using ionic liquid as adjuvant in aqueous two-phase system

Aziz, Nur Fazrin Husna Abdul; Abbasiliasi, Sahar; Ng, Hui Suan; Phapugrangkul, Pongsathon; Bakar, Mohamad Hafizi Abu; Tam, Yew Joon; Tan, Joo Shun

doi:10.1016/j.jchromb.2017.04.029

Cited by 34 publications

(19 citation statements)

References 22 publications

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“…However, due to the high solubility of most ILs in water at room temperature, usually large concentrations of salts are required to promote the ATPS formation, turning these processes more expensive and less sustainable. The use of small quantities of ILs as adjuvants in polymer-based ATPS appears as an alternative to overcome these difficulties [7,11,16–19]. …”

Section: Introductionmentioning

confidence: 99%

“…In addition to the previously described works and contrarily to those findings [7, 17, 20], in other works the use of ILs as adjuvants does not lead to the preferential migration of the target biomolecule to the polymer-rich phase or improvements on the partition coefficients. Aziz et al [19] used ATPS formed by PEGs of different molecular weights and potassium phosphate, potassium citrate and sodium acetate to extract β-mannanase. The authors showed that 3 wt% of [C 4 mim][BF 4 ] (1-butyl-3-methylimidazolium tetrafluoroborate) in the ATPS formulation enhances the β-mannanase partition to the salt-rich phase.…”

Section: Introductionmentioning

confidence: 99%

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Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents

Neves

Sousa

Pereira

et al. 2019

Biochemical Engineering Journal

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Ionic liquids (ILs) as adjuvants in polymer-salt aqueous two-phase systems (ATPS) have been used to improve the extraction of biomolecules. However, the impact of ILs as adjuvants on the partition of biomolecules is still poorly understood. Previous works mostly focused on ATPS based on strong salting-out agents, which may mask the IL effect. In this work, ATPS formed by polyethylene glycol (PEG 400) and a weak salting-out salt ((NH4)2SO4) with a wide number of ILs as adjuvants (chloride-based combined with cholinium, imidazolium, pyrrolidinium, piperidinium, tetralkylammonium and tetralkylphosphonium cations) were investigated. The respective phase diagrams were determined, and the systems extraction performance for a wide range of biomolecules (phenolic compounds, alkaloids and amino acids) was investigated. The results obtained show that ILs as adjuvants in polymer-salt ATPS modulate the partition of biomolecules. In particular, more hydrophobic ILs significantly enhance the partition of more hydrophobic biomolecules to the PEG-rich phase (where the IL is enriched). Furthermore, the intensity of the IL effect is more pronounced when using weak salting-out agents. A linear correlation between the biomolecules and the ILs partition coefficients, and with the biomolecules octanol-water partition coefficients, was found. In most ATPS formed by polymers and salts using ILs as adjuvants, the biomolecules partition is driven by the ILs partition and by the difference in hydrophobicity between the coexisting phases.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents

Neves

Sousa

Pereira

et al. 2019

Biochemical Engineering Journal

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“…The recovery performance of lipase in the desorption solution was evaluated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme sample was precipitated using TCA (Aziz et al 2017). The samples containing Laemmli buffer were boiled for 5 min and loaded into a polyacrylamide gel made up of 12% (v/v) resolving gel and electrophoresed at 140 V for 60 min.…”

Section: Methodsmentioning

confidence: 99%

Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system

et al. 2018

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This study aimed at recovery of thermostable lipase from BL21 using porous glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system (AIRS). The influencing parameters such as concentration and pH of extraction solution, concentration of NaCl, size of the beads, and pH of the desorption solution on the partition behaviour of lipase were evaluated. Smaller adsorbent (4 mm) had a 65.5% of recovery yield with approximately two-fold higher purification factor compared to that obtained with the larger adsorbent. Recombinant lipase was purified successfully using AIRS with a purification factor of 7.6 and yield of 78.4% under optimum conditions of 18% (w/w) PEG 4000, 10% (w/w) of potassium citrate at pH 9 with 3% (w/w) of NaCl. Optimum desorption was obtained with 4.0 mm of porous glass beads at pH 9.

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“…The basic unit of the peptide bond is amide. The amide I band, which falls between 1600-17000 cm -1 , is associated with the C=O stretching vibration of the amide functional group [1]. As the most sensitive spectral region to the secondary structure of the protein, this absorption band is often used as a structural probe to determine the structural properties of protein.…”

Section: Stability Of Bsa In 1-propanol/maltose Absmentioning

confidence: 99%

Efficacy of Alcohol/sugar Aqueous Biphasic System on Partition of Bovine Serum Albumin

Chow

Sahlini

et al. 2020

Preprint

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A green bio-separation alternative can be performed with a non-toxic and biodegradable aqueous biphasic system (ABS) composed of short-chain aliphatic alcohol-based top phase (1-propanol and 2-propanol) and carbohydrate-based bottom phase (glucose, sucrose, and maltose). A model protein, bovine serum albumin (BSA) was adopted to determine the effects of types and concentration of phase-forming components; protein concentration; and system pH on the protein partition efficiency in the ABS. Results showed that the 1-propanol/maltose ABS gave an overall better partition efficiency of BSA to the alcohol-rich top phase compared to the 1-propanol/sucrose ABS, 1-propanol/glucose ABS, and 2-propanol/sugar ABS attributed to the lower hydrophilicity of 1-propanol and the stronger sugaring-out effect exerted by the maltose. A maximum partition coefficient (K) of 20.01 ± 0.05 and recovery yield (Y) of 95.42% ± 0.01 of BSA were obtained with the 35% (w/w) 1-propanol/22% (w/w) maltose ABS at pH 5.0 which contained 10% (w/w) BSA. The K and Y of BSA in 1-propanol/maltose ABS was further enhanced with the addition of 3% (w/w) of ionic liquids, 1-butyl-3-methylimidazolium bromide ([Bmim]Br) as the adjuvants which provides the protein stabilizing effect. The Fourier Transform Infrared Spectrum (FTIR) analysis revealed that the protein structure remained unaltered upon the separation process.

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Purification of β -mannanase derived from Bacillus subtilis ATCC 11774 using ionic liquid as adjuvant in aqueous two-phase system

Cited by 34 publications

References 22 publications

Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents

Understanding the effect of ionic liquids as adjuvants in the partition of biomolecules in aqueous two-phase systems formed by polymers and weak salting-out agents

Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system

Efficacy of Alcohol/sugar Aqueous Biphasic System on Partition of Bovine Serum Albumin

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