Purification of Trichinella spiralis tubulin: comparison of several analytic procedures

González, Javier Martı́nez; González, Amparo Jiménez; Caabeiro, F. Rodriguez

doi:10.1016/s0304-4017(98)00098-3

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…A simple step to partial purification, as accomplished with DEAE anion-exchange chromatography, already showed appreciable results in immunoassay tests in other parasitic species studies to detect serum antibodies in Wuchereria bancrofti filariasis (Chenthamarakshan et al 1996) or for localization of fractionated antigen in Paragonimus infection (Lee and Chung, 2001); and also for Trichinela spiralis tubulin purification (Martínez-González et al 1998). Regarding diagnosis, the unbound fraction obtained in DEAE from heterologous filarial antigen provided better results in IgG detection for bancroftian filariasis (Chenthamarakshan et al 1996), in contrast with our results.…”

Section: Discussionmentioning

confidence: 99%

Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis

et al. 2012

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The aim of this study was to fractionate and partially characterize the antigenic extract of filariform larvae of Strongyloides venezuelensis in ion-exchange resin diethylaminoethyl sepharose (DEAE), to obtain antigenic fractions potentially applicable in immunoassays. Somatic antigen (SA) and its fractions DEAE S1 and DEAE S2 - which interacted with the resin - were evaluated by 1-dimensional electrophoresis to obtain protein profiles. SA and its fractions were tested in serum samples for IgG detection by ELISA. Serum samples (n = 155) were analysed: 50 from strongyloidiasis patients (G1), 55 from patients with other parasitic infections (G2) and 50 from healthy volunteers. Sensitivity (Se), specificity (Sp), area under curve (AUC) and likelihood ratios (LR) were calculated. The DEAE S2 fraction provided a high diagnostic value for IgG detection (Se 92·0%, Sp 91·4%, AUC 0·981, LR+ 10·75, LR - 0·09). In conclusion, the DEAE S2 fraction would probably be a source of immunodominant polypeptides for IgG detection in human strongyloidiasis serodiagnosis.

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