Purification of the phytase enzyme fromLactobacillus plantarum: The effect on pansy growth and macro–micro element content

Abstract

In the present study, the phytase enzyme was purified from Lactobacillus plantarum with a 3.08% recovery, 9.57-purification fold, and with a specific activity of 278.82 EU/mg protein. Then, the effects of the 5 EU and 10 EU purified phytase was determined on the plant growth, quality, the macro-micro nutrient content of pansy (Viola × wittrockiana), which is of great importance in ornamental plants industry. The research was established under greenhouse conditions with natural light in 2017. The pansy seeds were coated with phytase enzyme solution, sown in a peat environment, and transferred to pots at the seedling period. In general, the 5 EU and 10 EU applications increase plant height, the number of leaves per plant, the number of side branches per plant, and flower height parameters compared to control. Also, microand macronutrient values in soil and plant samples were examined. According to the results, the phytase application on pansy cultivation positively affected the properties and yielded high quality of plants.

Ornamental cabbage (Brassica oleracea var. acephala) responses to phytase enzyme purified from Lactobacillus coryniformis application

“…This situation can be explained by better nutrition of plants and the research results of Chen et al .46 It has been reported as a result of previous study that the 5 EU and 10 EU applications of the phytase enzyme increased in plant height of pansy. 15 Also, Rahi et al 47 stated that the production of phytase, synthesis of auxins, solubilization of siderophores and inorganic phosphates had positive effect of plant growth promoting, when Lotus japonicus seedlings were co-cultured with a fungus (Discosia sp. FIHB 571 isolate).…”

“…Microbial enzymes are day by day employed due to their catalytic activity, environmentally friendly features, low cost, and their high yields with low side products in agricultural production. [13][14][15] Enzymes are produced by cellular anabolism, the naturally occurring biological process to make more complex molecules from simpler ones. 16,17 Agricultural enzymes can be defined as biocatalysts that accelerate chemical reactions by breaking down the nutrients in the soil to be easily absorbed by plant roots.…”

“…In the literature, except for the study by Parlakova Karagöz et al 15 there is no study evaluating the potential of using phytase from a microorganism in the cultivation of ornamental plants. In previous studies, microorganisms capable of synthesizing phytase were directly grafted onto the seed or root zone.…”

“…In previous studies, microorganisms capable of synthesizing phytase were directly grafted onto the seed or root zone. Parlakova Karagöz et al 15 investigated the effects of different levels of phytase purified from microorganisms in pansy cultivation. In our present study, both different phytase levels and the effect of different soaking times on seed inoculation of phytase were evaluated.…”

Aspergillus niger Enhances Organic and Inorganic Phosphorus Release from Wheat Straw by Secretion of Degrading Enzymes and Oxalic Acid

“…Straw returning has been widely practiced in agricultural production systems to alleviate soil degradation and maintain soil fertility. , Long-term straw returning increases nitrogen (N), phosphorus (P), and potassium (K) contents in the soil, reducing the dependence on fertilizers . In terms of P, straw returning not only reduces P sorption in soil but also increases inorganic and organic P contents .…”