Purification of the Associated 3-dehydroquinate Hydrolyase and Shikimate Oxidoreductase in Spinach Chloroplasts

Localization, Purification, and Characterization of Shikimate Oxidoreductase-Dehydroquinate Hydrolyase from Stroma of Spinach Chloroplasts

“…The two enzymic activities were found exclusively in the stroma ofspinach chloroplasts as demonstrated in this paper and the preceding abstract (13). To understand the structural/functional relationships ofthe multiple forms requires the ability to isolate them in a purified state. We have improved the previously reported purification procedure (12) to achieve the apparent homogeneity and stability which was necessary for the characterization of SORase/ DHQase. The purification procedure of a shikimate pathway enzyme from the chloroplast stroma is described here for the first time.…”