Purification of Staphylococcal Alpha Toxin by Electrofocusing

Goode, Ronald L.; Baldwin, Jack N.

doi:10.1080/00327487308061520

Cited by 5 publications

(5 citation statements)

References 6 publications

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“…The sedimentation coefficient of alpha hemolysin is in the range 2.8S to 3.1S (9,31,32,72,125,175,176). Another small fast-moving peak of 12S to 16S was recorded by Bernheimer and Wiseman et al (217) Goode and Baldwin (72) Wood 46 A, C, C, G 1.8 x 104 Watanabe and Kato (203) Wood 46 A, B, C, C, D, C…”

Section: Bacteriol Revmentioning

confidence: 89%

“…Alpha A and alpha I are the main com-1nents with the same pI, and are reputed to ,OWN interconversion to alpha B and alpha IV, 0pectively. Other investigators (56,72,73) 1ve also noted pl values in the region of 8.5. E and Haque 2 have described an alpha and pha-1 hemolysin on the basis of results obined with Haque's electrophoretic localiza-)n technique. One (alpha) lysed erythrocytes rabbits, sheep, and horses whereas the other Ipha-1) lysed only those of the rabbit.…”

mentioning

confidence: 86%

“…Ganczarski (65) labeled alpha heool. ysin with '4CO2, and found that CO2 fixation might play a role in the forrnation of a key amino acid essential for hemolysin production Goode and Baldwin (72) have claimed high yields (2,500 hemolytic units/ml) of the hemolysin in thoroughly aerated Trypticase soy broth supplemented with yeast extract. They fOunld in contrast with many other workers that Co2 did not enhance titers.…”

Section: Bacteriol Revmentioning

confidence: 99%

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The hemolysins of Staphylococcus aureus.

Wiseman¹

1975

Bacteriological Reviews

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Section: Bacteriol Revmentioning

confidence: 89%

mentioning

confidence: 86%

Section: Bacteriol Revmentioning

confidence: 99%

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The hemolysins of Staphylococcus aureus.

Wiseman¹

1975

Bacteriological Reviews

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“…Media and growth conditions. The media and growth conditions have been described previously (5). Trypticase soy broth (Baltimore Biological Laboratories) containing 0.3% Difco yeast extract (TSY) in 400-ml quantities in 12 1-liter flasks was inoculated with 5-ml quantities of an overnight TSY broth culture and incubated at 37 C with shaking for 20 h.…”

Section: Methodsmentioning

confidence: 99%

“…Purification of toxin. The purification procedure for staphylococcal alpha-toxin has been described (5). Proteins were precipitated by saturation of concen-'Present address: Hoffman LaRoche Inc., Nutley, N. J.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Purified Alpha-Toxins from Various Strains of Staphylococcus aureus

Goode¹,

Baldwin²

1974

Applied Microbiology

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Alpha-toxin from five strains of Staphylococcus aureus, including Wood 46, was purified by isoelectric focusing. The alpha-toxins obtained from different strains were similar. The isoelectric point of the purified toxins was 8.65 I 0.15. Sharp concentration peaks were not always obtained. In the ultracentrifuge the alpha-toxins migrated usually as three peaks which could be dissociated with propionic acid to yield one peak. A single line of identity was obtained in immunoelectrophoresis when a heterologous antiserum was reacted with the five purified toxins. It was concluded that the widespread use of the Wood 46 strain for the production of alpha-toxin is justified.

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The nature of the competitive ability of spontaneous staphylocoagulase-negative mutants of Staphylococcus aureus with respect to growth of the parent strains in continuous culture

Engels

Kamps

1982

Antonie van Leeuwenhoek

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During prolonged cultivation of S. aureus strains 104 and NCTC 8178 in continuous culture, staphylocoagulase-negative mutants arose and accumulated progressively in increasing proportions. The resulting loss of production of staphylocoagulase was accompanied by a simultaneous loss of production of alpha-haemolysin and PV-leucocidin. Characterization of the strains revealed no further difference in biotype, exoenzymes phage pattern and plasmid content. Cultivation in batch cultures showed that the maximal specific growth rates and specific oxygen-consumption rates of the mutant strains were slightly higher than those of the parent strains, whereas the production of total extracellular protein of the mutant strains had decreased significantly. From competition experiments between parent and mutant strains in chemostat cultures at different dilution rates and cultivation temperatures, it was concluded that the underlying mechanism of accumulation of staphylocoagulase-negative mutants in the chemostat is based on differences in affinity for the limiting substrate(s) rather than on differences in the production rates of total extracellular proteins. The complete repression of three exoenzymes, an partial repression of the total extracellular protein production, and an increased affinity for the limiting substrate(s) suggested that a mutation in a regulatory gene is involved. The possible role of a transposon in this mutation is discussed.

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Purification of Staphylococcal Alpha Toxin by Electrofocusing

Cited by 5 publications

References 6 publications

The hemolysins of Staphylococcus aureus.

The hemolysins of Staphylococcus aureus.

Comparison of Purified Alpha-Toxins from Various Strains of Staphylococcus aureus

The nature of the competitive ability of spontaneous staphylocoagulase-negative mutants of Staphylococcus aureus with respect to growth of the parent strains in continuous culture

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