Purification of sporulation and swarming inhibitors from Ulva

Kessler, Ralf W.; Alsufyani, Taghreed; Wichard, Thomas

doi:10.1201/b21460-8

Cited by 9 publications

(5 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…is to obtain and concentrate viable swarmers (gametes or spores) to be seeded on suitable growth substrates. Using an established protocol for U. mutabilis [55], we could successfully manipulate gametangia induction and swarmer release in Swedish U. fenestrata which guarantees the independence of natural, seasonal reproduction patterns and further supports a profitable large-scale cultivation. Our results further showed that a high seeding density is favored in order to achieve a commercially viable large-scale cultivation of U. fenestrata and growth at scale.…”

Section: Biomass Yield Growth and Performancementioning

confidence: 90%

Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm

Steinhagen

Enge

Larsson

et al. 2021

JMSE

View full text Add to dashboard Cite

The growing world population demands an increase in sustainable resources for biorefining. The opening of new farm grounds and the cultivation of extractive species, such as marine seaweeds, increases worldwide, aiming to provide renewable biomass for food and non-food applications. The potential for European large-scale open ocean farming of the commercial green seaweed crop Ulva is not yet fully realized. Here we conducted manipulative cultivation experiments in order to investigate the effects of hatchery temperature (10 and 15 °C), nutrient addition (PES and 3xPES) and swarmer density (500 and 10,000 swarmers ml−1) on the biomass yield and biochemical composition (fatty acid, protein, carbohydrate, pigment and phenolic content) of off-shore cultivated Ulva fenestrata in a Swedish seafarm. High seedling densities were optimal for the growth of this northern hemisphere crop strain and significantly increased the mean biomass yield by ~84% compared to low seedling densities. Variations of nutrients or changes in temperature levels during the hatchery phase were not necessary to increase the subsequent growth in an open-water seafarm, however effects of the factors on the thallus habitus (thallus length/width) were observed. We found no significant effect of the environmental factors applied in the hatchery on the total fatty acid or crude protein content in the off-shore cultivated Ulva. However, low seedling density and low temperature increased the total carbohydrate content and furthermore, high temperature in combination with high nutrient levels decreased the pigment content (chlorophyll a, b, carotenoids). Low temperature in combination with high nutrient levels increased the phenolic content. Our study confirms the successful and sustainable potential for large-scale off-shore cultivation of the Scandinavian crop U. fenestrata. We conclude that high seedling density in the hatchery is most important for increasing the total biomass yield of sea-farmed U. fenestrata, and that changing temperature or addition of nutrients overall does not have a large effect on the biochemical composition. To summarize, our study contributes novel insights into the large-scale off-shore cultivation potential of northern hemisphere U. fenestrata and underpins suitable pre-treatments during the hatchery phase of seedlings to facilitate a successful and cost-efficient large-scale rope cultivation.

show abstract

Section: Biomass Yield Growth and Performancementioning

confidence: 90%

Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm

Steinhagen

Enge

Larsson

et al. 2021

JMSE

View full text Add to dashboard Cite

show abstract

“…, Kessler et al. ). Swarming inhibitors were removed after 3 d by rewashing the material as described by Wichard and Oertel ().…”

Section: Methodsmentioning

confidence: 99%

Conspecificity of the model organism Ulva mutabilis and Ulva compressa (Ulvophyceae, Chlorophyta)

Steinhagen

Barco

Wichard

et al. 2018

Journal of Phycology

Self Cite

View full text Add to dashboard Cite

As one of the most abundant and ubiquitous representatives of marine and brackish coastal macrophytobenthos communities, the genus Ulva is not only an important primary producer but also of ecological and morphogenetic interest to many scientists. Ulva mutabilis became an important model organism to study morphogenesis and mutualistic interactions of macroalgae and microorganisms. Here, we report that our collections of Ulva compressa Linnaeus (1753) from Germany are conspecific with the type strains of the model organism U. mutabilis Føyn (1958), which were originally collected at Olhão on the south coast of Portugal and have from that time on been maintained in culture as gametophytic and parthenogenetic lab strains. Different approaches were used to test conspecificity: (i) comparisons of vegetative and reproductive features of cultured material of U. mutabilis and German U. compressa demonstrated a shared morphological pattern; (ii) gametes of U. compressa and U. mutabilis successfully mated and developed into fertile sporophytic first‐generation offspring; (iii) molecular phylogenetics and species delimitation analyses based on the Generalized Mixed Yule‐Coalescent method showed that U. mutabilis isolates (sl‐G[mt+]) and (wt‐G[mt‐]) and U. compressa belong to a unique Molecular Operational Taxonomic Unit. According to these findings, there is sufficient evidence that U. mutabilis and U. compressa should be regarded as conspecific.

show abstract

“…The formation of spores in more than half a disc was considered equivalent to the complete phase transition. 8,23 The InfoStat software was used to apply oneway ANOVA, wherein a post hoc test was performed to measure the phase change after 24 h and 48 h.…”

Section: Effects Of Different Abiotic Conditions On Cells Undergoing ...mentioning

confidence: 99%

A metabolomic snapshot through NMR revealed differences in phase transition during the induction of reproduction in Ulva ohnoi (Chlorophyta)

Bodar,

Thakur,

Rajai

et al. 2024

Mol. Omics

View full text Add to dashboard Cite

show abstract

Purification of sporulation and swarming inhibitors from Ulva

Cited by 9 publications

References 1 publication

Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm

Sustainable Large-Scale Aquaculture of the Northern Hemisphere Sea Lettuce, Ulva fenestrata, in an Off-Shore Seafarm

Conspecificity of the model organism Ulva mutabilis and Ulva compressa (Ulvophyceae, Chlorophyta)

A metabolomic snapshot through NMR revealed differences in phase transition during the induction of reproduction in Ulva ohnoi (Chlorophyta)

Contact Info

Product

Resources

About