Purification of Soluble β-Glucan with Immune-enhancing Activity from the Cell Wall of Yeast

Lee, Jung Nam; Lee, Do-Youn; Ji, In Hye; Kim, Gi Eun; Kim, Hye Nam; Sohn, Jeongwon; Kim, Sangduk; Kim, Chan Wha

doi:10.1271/bbb.65.837

Cited by 93 publications

(65 citation statements)

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“…Several extraction techniques are there to recover the potent BG from microbial and plant sources, but the choice of suitable extraction method plays a vital role that will affect the structure, quality, and functionality of the BG (Ahmad et al, 2012 from cytoplasm) and BG extraction (extraction from insoluble cell wall) (Javmen et al, 2012). Several studies reported about chemical (NaDH, HCl, acetic acid, citric acid) (Lee et al, 2001;Hunter et al, 2002;Pelizon et al, 2005), physical (sonication, high pressure) (Boonraeng et al, 2000;Shokri et al, 2008;Wenger et al, 2008) and enzymatic (lytic enzymes) lysis of yeast cells (Ferrer, 2006).…”

Section: Introductionmentioning

confidence: 99%

Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice

et al. 2016

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Beta-glucan (BG) is a conserved cell wall components of bacteria, fungi, and yeast. BG is an immunomodulator and stimulates the host immune system. This study was performed to screen Saccharomyces cerevisiae strain with high BG, extraction of BG using different chemical extraction methods, composition analysis of BG, and evaluation of the immunomodulatory effect of high-quality BG using mice model. Ten yeast strains were screened for high BG content using total glucan extraction kit and were subjected to FT-IR analysis. The kit based extraction revealed that HII31 showed a high content of total glucan and BG. HII31 cells were subjected to four different acid/base extractions, which indicated that combination of a strong base (NaDH) and weak acid (CH 3 CDDH) extraction recovered high BG and a high ratio of polysaccharide, protein, and lipid. Further, the immunomodulatory effect of the selected BG was evaluated using mice, which suggested that low dose of HII31-BG induces the expression of selected pro-inflammatory (IL-17, IFN-γ) and anti-inflammatory cytokine (IL-10) significantly, whereas relatively high dose was required to alter the IL-6 and TGF-β expression. Dverall, the present study revealed that BG extracted from HII31 cells alters the expression of studied cytokines, which can be used as a potent immunomodulator in pharmaceutical products.Keywords: beta-glucan; Saccharomyces cerevisiae; immunomodulation; FTIR; cytokines.Practical Application: Identification of optimum extraction procedure for β-glucan (BG) from S. cerevisiae HII31 and revealed that BG can be used as a potent immune modulator in pharmaceutical products.

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Section: Introductionmentioning

confidence: 99%

Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice

et al. 2016

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“…It has been suggested that signals from (1,3)-␤-D-glucan are transmitted through heterodimers of Toll-like receptor 2 (TLR-2) and TLR-6 (2,27), although these are thought to contain multiple stimulators for macrophages in addition to (1,3)-␤-D-glucan (46). Cellulose (1,4)-␤-D-glucan is a polysaccharide produced by plant cells, fungi, and bacteria (21,23,25,34,35). We previously found that AC-1, an extracellular polysaccharide produced by Acetobacter xylinum and composed of (1,4)-␤-Dglucan with branches of glucosyl residues, showed a strong activity to induce production of interleukin-12 (IL-12) p40 and tumor necrosis factor alpha (TNF-␣) by macrophage cell lines in vitro via Toll-like receptor 4 (TLR-4) signaling (23,32,34,35).…”

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Immunostimulating Properties of Intragastrically AdministeredAcetobacter-Derived Soluble Branched (1,4)-β-d-Glucans Decrease Murine Susceptibility toListeria monocytogenes

Yajima

Saito

et al. 2004

Infect Immun

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We previously found that AC-1, an extracellular polysaccharide, produced by Acetobacter xylinum and composed of (1,4)-␤-D-glucan with branches of glucosyl residues, showed a strong activity to induce production of interleukin-12 (IL-12) p40 and tumor necrosis factor alpha by macrophages in vitro via Toll-like receptor 4 (TLR-4) signaling. In the present study, we examined the effect of oral administration of AC-1 on protective immunity against Listeria monocytogenes. Mice were given AC-1 or phosphate-buffered saline (PBS) intragastrically 2 days before, on the day of, and 2 days after an intraperitoneal inoculation of L. monocytogenes. The survival rate of AC-1-treated mice was significantly improved and bacterial growth in AC-1-treated mice was severely retarded compared to those of PBS-treated mice after infection with L. monocytogenes. IL-12 p40 levels in serum and magnitudes of CD4 ؉ Th1 and CD8 ؉ Tc1 responses against Listeria antigen were significantly higher in AC-1-treated mice than in PBS-treated mice. The effect of AC-1 on antilisterial activity was diminished in C3H/HeJ mice carrying mutated TLR-4. Thus, AC-1, a potent IL-12 inducer through TLR-4, enhanced protective immunity against L. monocytogenes via augmentation of Th1 responses. These results suggest that infectious processes driven by intracellular microorganisms could be prevented to develop by the (1,4)-␤-D-glucan.

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“…En effet, des études aussi bien anciennes que récentes ont montré l'implication des extraits de paroi de levure S. cerevisiae, tel que le Zymosan, dans le système immunitaire (Miura et al, 1999). D'autres travaux ont signalé que ce sont en fait les β-glucanes de la paroi qui sont responsables de la stimulation du système immunitaire (Lee et al, 2001), probablement à travers leurs effets sur l'activation des macrophages (Herich et Levkut, 2002 ;Sarazin et al, 2010). En outre, les mannanes de la paroi de levure seraient capables de stimuler le système immunitaire chez le rat (Ataoglu et al, 2000).…”

Section: ■ Resultatsunclassified

Effet immunostimulant de quatre additifs alimentaires contre les maladies de Gumboro et de Newcastle chez le poulet de chair (<em>Gallus gallus</em>)

Azeroual

Mesfioui

Bouzoubaa

et al. 2016

Rev. Elev. Med. Vet. Pays Trop.

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de Gumboro appelée aussi bursite infectieuse [infectious bursal disease (IBD)] et la maladie de Newcastle [Newcastle disease (ND)], dénommée également pseudo-peste aviaire, détiennent respectivement la deuxième (8 %) et la troisième place (6 %), après la colibacillose (Mouahid et Bouzoubaa, 2001). De même, ces pathologies se placent en tête des maladies aviaires les plus contagieuses et dévasta-trices et ont un impact socio-économique extrêmement important au niveau international (van der Berg et al., 2000 ;van der Sluis, 1999 ; OIE, 2012). Les épidémies surviennent surtout chez les poulets non vaccinés causant 100 % de morbidité et 25 % de mortalité (Shabbir et al., 2008). Cependant, ces maladies surviennent même dans les troupeaux vaccinés en raison de la défaillance d'interventions et de vaccins immunitaires inappropriés (Qayyum et al., 2012).Ainsi, le renforcement du système immunitaire chez ces espèces génétiquement fragilisées est devenu une pratique indispensable pour ■ INTRODUCTION L'analyse des pathologies aviaires diagnostiquées au Maroc a montré la prédominance des pathologies d'origine infectieuses chez toutes les espèces de production, particulièrement chez la poule pondeuse (86 %), la poule reproductrice (85 %) et le poulet de chair (69 %). Chez ce dernier, les pathologies virales, en l'occurrence la maladie ■ SANTÉ ANIMALE ET ÉPIDÉMIOLOGIE 185Revue d'élevage et de médecine vétérinaire des pays tropicaux, 2015, 68 (4) : 185-189

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Purification of Soluble β-Glucan with Immune-enhancing Activity from the Cell Wall of Yeast

Cited by 93 publications

References 16 publications

Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice

Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice

Immunostimulating Properties of Intragastrically AdministeredAcetobacter-Derived Soluble Branched (1,4)-β-d-Glucans Decrease Murine Susceptibility toListeria monocytogenes

Effet immunostimulant de quatre additifs alimentaires contre les maladies de Gumboro et de Newcastle chez le poulet de chair (<em>Gallus gallus</em>)

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