Purification of rat hepatic stellate cells by side scatter-activated cell sorting

Geerts, Albert; Niki, Toshiro; Hellemans, Karine; Craemer, Dirk De; Berg, Kit Van Den; Lazou, Jean-Marc; Stangé, Geert; Winkel, M. Van De; Bleser, Pieter De

doi:10.1002/hep.510270238

Cited by 101 publications

(96 citation statements)

References 34 publications

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“…Isolation and Purification of Liver Cells-Hepatic sinusoidal cells (stellate cells, sinusoidal endothelial cells, and Kupffer cells) from murine liver were isolated and cultured based on a method described previously (20). Briefly, murine livers were perfused with 0.02% collagenase, 0.04% Pronase and further digested in vitro with 0.04% collagenase, 0.04% Pronase, and 0.001% DNase for 20 min at 37°C under constant stirring.…”

Section: Methodsmentioning

confidence: 99%

Peroxisomal and Microsomal Lipid Pathways Associated with Resistance to Hepatic Steatosis and Reduced Pro-inflammatory State

Hall

Poussin

Velagapudi

et al. 2010

Journal of Biological Chemistry

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Accumulation of fat in the liver increases the risk to develop fibrosis and cirrhosis and is associated with development of the metabolic syndrome. Here, to identify genes or gene pathways that may underlie the genetic susceptibility to fat accumulation in liver, we studied A/J and C57Bl/6 mice that are resistant and sensitive to diet-induced hepatosteatosis and obesity, respectively. We performed comparative transcriptomic and lipidomic analysis of the livers of both strains of mice fed a high fat diet for 2, 10, and 30 days. We found that resistance to steatosis in A/J mice was associated with the following: (i) a coordinated up-regulation of 10 genes controlling peroxisome biogenesis and ␤-oxidation; (ii) an increased expression of the elongase Elovl5 and desaturases Fads1 and Fads2. In agreement with these observations, peroxisomal ␤-oxidation was increased in livers of A/J mice, and lipidomic analysis showed increased concentrations of long chain fatty acid-containing triglycerides, arachidonic acid-containing lysophosphatidylcholine, and 2-arachidonylglycerol, a cannabinoid receptor agonist. We found that the anti-inflammatory CB2 receptor was the main hepatic cannabinoid receptor, which was highly expressed in Kupffer cells. We further found that A/J mice had a lower proinflammatory state as determined by lower plasma levels and IL-1␤ and granulocyte-CSF and reduced hepatic expression of their mRNAs, which were found only in Kupffer cells. This suggests that increased 2-arachidonylglycerol production may limit Kupffer cell activity. Collectively, our data suggest that genetic variations in the expression of peroxisomal ␤-oxidation genes and of genes controlling the production of an anti-inflammatory lipid may underlie the differential susceptibility to diet-induced hepatic steatosis and pro-inflammatory state.The current view of obesity-associated metabolic deregulations proposes that excessive accumulation of fat in adipose tissue initiates an inflammatory reaction characterized by cytokine production by adipocytes and infiltrating leukocytes (1, 2). This leads to the development of insulin resistance in adipocytes and increased release of free fatty acids. Secreted cytokines and free fatty acids then induce insulin resistance in muscle and liver, through activation of serine/threonine kinases that phosphorylate essential signaling proteins such as the insulin receptor or its main substrates IRS1 and IRS2 (3). In the liver, insulin resistance leads to unrestrained glucose production causing hyperglycemia. However, insulin still normally activates the lipogenic transcription factor SREBP-1c leading to hepatosteatosis, increased secretion of VLDL, and elevated plasma triglycerides (4). Therefore, there is a correlation between the degree of inflammation in the adipose tissue and liver fat content and insulin resistance (5), and studies in human have demonstrated a correlation between the level of fat accumulation in the liver and various parameters of the metabolic syndrome (6, 7).Changes in liver metaboli...

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Section: Methodsmentioning

confidence: 99%

Peroxisomal and Microsomal Lipid Pathways Associated with Resistance to Hepatic Steatosis and Reduced Pro-inflammatory State

Hall

Poussin

Velagapudi

et al. 2010

Journal of Biological Chemistry

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show abstract

“…This was loaded onto a 17.2% Nycodenz solution and covered the layer with 1 ml of HBSS. Following centrifugation for 15 min at 3,000 g, the cells at the top of the Nycodenz were collected, and washed by centrifugation (Hendriks et al, 1985;Vyas et al, 1995;Geerts et al, 1998).…”

Section: Cell Isolationmentioning

confidence: 99%

Cell cycle protein profile of the hepatic stellate cells (HSCs) in dimethylnitrosamine-induced rat hepatic fibrosis

Kim

Kim²,

Lee³

et al. 2005

Exp Mol Med

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“…After 2 days, cell debris and nonadherent cells were removed by washing and the medium was changed every 2 or 3 days thereafter. Cells cultured for 2 days after isolation represented quiescent HSC, whereas those cultured for 10 days after isolation represented activated HSC (26).…”

Section: In Vitro Experimentsmentioning

confidence: 99%

Successful Targeting to Rat Hepatic Stellate Cells Using Albumin Modified with Cyclic Peptides That Recognize the Collagen Type VI Receptor

Beljaars¹,

Molema²,

Schuppan³

et al. 2000

Journal of Biological Chemistry

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105

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Purification of rat hepatic stellate cells by side scatter-activated cell sorting

Cited by 101 publications

References 34 publications

Peroxisomal and Microsomal Lipid Pathways Associated with Resistance to Hepatic Steatosis and Reduced Pro-inflammatory State

Peroxisomal and Microsomal Lipid Pathways Associated with Resistance to Hepatic Steatosis and Reduced Pro-inflammatory State

Cell cycle protein profile of the hepatic stellate cells (HSCs) in dimethylnitrosamine-induced rat hepatic fibrosis

Successful Targeting to Rat Hepatic Stellate Cells Using Albumin Modified with Cyclic Peptides That Recognize the Collagen Type VI Receptor

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