Purification of rabies virus from tissue culture

Schneider, Lothar; Horzinek, Marian C.; Matheka, H. D.

doi:10.1007/bf01242982

Cited by 16 publications

(10 citation statements)

References 10 publications

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“…Several passages are required to select a fixed strain that is adapted to the multiplication in non-neuronal cell lines (such as BHK21 and Vero cells) [31,32,33]. Most of the fixed RABV laboratory strains have resulted from such an adaptation process.…”

Section: Rhabdovirus Receptorsmentioning

confidence: 99%

Molecular and Cellular Aspects of Rhabdovirus Entry

Albertini

Baquero

Ferlin

et al. 2012

Viruses

130

106

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Rhabdoviruses enter the cell via the endocytic pathway and subsequently fuse with a cellular membrane within the acidic environment of the endosome. Both receptor recognition and membrane fusion are mediated by a single transmembrane viral glycoprotein (G). Fusion is triggered via a low-pH induced structural rearrangement. G is an atypical fusion protein as there is a pH-dependent equilibrium between its pre- and post-fusion conformations. The elucidation of the atomic structures of these two conformations for the vesicular stomatitis virus (VSV) G has revealed that it is different from the previously characterized class I and class II fusion proteins. In this review, the pre- and post-fusion VSV G structures are presented in detail demonstrating that G combines the features of the class I and class II fusion proteins. In addition to these similarities, these G structures also reveal some particularities that expand our understanding of the working of fusion machineries. Combined with data from recent studies that revealed the cellular aspects of the initial stages of rhabdovirus infection, all these data give an integrated view of the entry pathway of rhabdoviruses into their host cell.

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Section: Rhabdovirus Receptorsmentioning

confidence: 99%

Molecular and Cellular Aspects of Rhabdovirus Entry

Albertini

Baquero

Ferlin

et al. 2012

Viruses

130

106

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“…However, viruses can be adapted (Kissling, 1958) and several passages are required for the virus to be adapted fully to the in vitro multiplication. Additional cycles of multiplication in non‐neuronal cells are necessary for the selection of fixed strains that would multiply in established cell lines such as BHK21, BSR and Vero cells (Wiktor et al ., 1964; Schneider et al ., 1971). Evelyn Rokitnicki Abelseth (ERA), Pasteur Virus (PV) or Challenge Virus Standard (CVS) are fixed RV strains that have been selected in the past according to this procedure and are used around the world for laboratory investigation.…”

Section: Introductionmentioning

confidence: 99%

Low-affinity nerve-growth factor receptor (P75NTR) can serve as a receptor for rabies virus

et al. 1998

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A random-primed cDNA expression library constructed from the mRNA of neuroblastoma cells (NG108) was used to clone a specific rabies virus (RV) receptor. A soluble form of the RV glycoprotein (G s ) was utilized as a ligand to detect positive cells. We identified the murine low-affinity nerve-growth factor receptor, p75NTR. BSR cells stably expressing p75NTR were able to bind G s and G-expressing lepidopteran cells. The ability of the RV glycoprotein to bind p75NTR was dependent on the presence of a lysine and arginine in positions 330 and 333 respectively of antigenic site III, which is known to control virus penetration into motor and sensory neurons of adult mice. P75NTR-expressing BSR cells were permissive for a non-adapted fox RV isolate (street virus) and nerve growth factor (NGF) decreased this infection. In infected cells, p75NTR associates with the RV glycoprotein and could be precipitated with anti-G monoclonal antibodies. Therefore, p75NTR is a receptor for street RV.

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“…We have also found that rabies virus and other rhabdoviruses may be efficiently concentrated by ammonium sulfate precipitation (1). Recently Schneider et al (11) used the aluminum phosphate gel adsorption technique (9) to concentrate and purify rabies virus. Rabies virus thus concentrated was also an efficient immunogen.…”

mentioning

confidence: 77%