Purification of polysaccharide from artificially cultivated Anoectochilus roxburghii (wall.) Lindl . by high-speed counter current chromatography and its antitumor activity

Abstract

To establish a systematic method for the extraction, purification, characterization and antitumor activity study of polysaccharide from artificially cultivated Anoectochilus roxburghii (wall.) Lindl. (AC-ARPS). High-speed counter current chromatography with two-phase aqueous systems was successfully applied to purify AC-ARPS after one-step separation. The purity of the AC-ARPS obtained by phenol/sulfuric acid method was 95.01%. The chemical structures of AC-ARPS were identified by a series of analytical methods including high-performance liquid chromatography and liquid chromatography with mass spectrometry. High-performance liquid chromatography and liquid chromatography with mass spectrometry indicated that AC-ARPS was mainly composed of mannose, ribose, glucose, galactose and arabinose with a molar ratio of 1.00:8.47:47.30:1.17:1.19. AC-ARPS is a homogeneous polysaccharide with a molecular weight of 25 681 Da. The antitumor effect of AC-ARPS was evaluated on lung cancer A549, osteosarcoma 143B, rat adrenal pheochromocytoma PC 12, breast cancer MCF-7, acute leukemia HL 60, chronic leukemia K562, colon cancer SW620, esophageal cancer OE 19, liver cancer HepG2, and neuroglioma U251 cells in vitro. AC-ARPS showed the best inhibitory effect on OE 19 cells, and the IC value was 5.67 ± 0.831 μmol/L. Fluorescence analysis and flow cytometry results showed that AC-ARPS induced apoptosis and G2/M phase arrest in OE 19 cells.

Hepatoprotective Effects of Polysaccharide from Anoectochilus roxburghii (Wall.) Lindl. on Rat Liver Injury Induced by CCl4

“…4 In recent decades, many studies have shown that A. roxburghii, as a Chinese folk medicine, has diverse pharmacological effects and medicinal properties, such as hepatoprotection, [5][6][7][8][9] anti-diabetes, 10,11 antifatigue, 12 antiosteoporosis, 9,13,14 vascular protective, 15 antioxidation, 16,17 and antitumor. 18,19 Some scholars have applied advanced and efficient technologies in the extraction, separation, and structural characterization of the active ingredients from A. roxburghii because of its curative effects. In recent years, the research team of the authors has conducted an in-depth exploration of the active components of A. roxburghii.…”

“…10,27,28 For example, the polysaccharide from artificially cultivated A. roxburghii has been reported to possess antitumor effects. 19 However, little information has been published in terms of purification and structural characterization of the polysaccharide from A. roxburghii and its effects on CCl 4induced liver injury by in rats thus far. Some reports are available on the hepatoprotective effects of A. roxburghii from the different genera of the same family.…”

“… 10 , 27 , 28 For example, the polysaccharide from artificially cultivated A. roxburghii has been reported to possess antitumor effects. 19 …”

Detecting kinsenoside from Anoectochilus roxburghii by HPLC‐ELSD with dual columns of NH2 and AQ‐C18

“…Anoectochilus roxburghii contains flavonoids, 3,4 polysaccharides, 5 kinsenoside, 6 alkaloids, 7 sterols, 8 organic acids, 9 amino acids, trace elements, cardiac glycosides, and other active ingredients 10,11 . This herb is also referred to as ‘Golden Grass,’ ‘King of Medicine,’ and ‘Magic pill’ because of its diverse pharmacological effects, including antihyperglycemia, 12 antitumor, 13 anti‐inflammatory, 14,15 antioxidation, 16,17 and hepatoprotection 18,19 …”

Regulation of flowering time using temperature, photoperiod and spermidine treatments in Anoectochilus roxburghii

“…Therefore, it is extremely economically valuable (Ye et al 2017). It is not only one of the original plants used in Traditional Chinese Medicine for diabetes (Cui et al 2012), tumors (Yu et al 2017), hyperpiesia (An et al 2014) and hepatitis (Zeng et al 2017), but it also has ornamental, cosmetological, culinary and other beneficial uses (Zhang et al 2015;Shao et al 2014). And aromatic and medicinal plants have been used for therapeutic, religious, cosmetic, nutritional, and beautification purposes since ancient times and humanity of all civilizations and culture are familiar with their usage (Ersoy et al 2017;Abramovic et al 2018;Tiwari et al 2018).…”