Purification of plasmid DNA by tangential flow filtration

Kahn, David W.; Butler, Michelle D.; Cohen, Darien L.; Gordon, Margaret Jane; Kahn, Jeanne; Winkler, Moritz

doi:10.1002/(sici)1097-0290(20000705)69:1<101::aid-bit12>3.0.co;2-1

Cited by 72 publications

(33 citation statements)

References 19 publications

(22 reference statements)

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“…The samples were then evaporated to dryness and redissolved in double distilled water. Samples were desalted by TFF (tangential flow filtration), using the Pall Filtron Omega TM 100 VR (Kahn et al 2000). To resolve the D/L isomers by GC, the Chirasil-Val capillary column (Alltech, USA), was used.…”

Section: Methodsmentioning

confidence: 99%

Techniques of DNA-studies on prehispanic ectoparasites (Pulex sp., Pulicidae, Siphonaptera) from animal mummies of the Chiribaya Culture, Southern Peru

Dittmar

Mamat

Whiting

et al. 2003

Mem. Inst. Oswaldo Cruz

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Section: Methodsmentioning

confidence: 99%

Techniques of DNA-studies on prehispanic ectoparasites (Pulex sp., Pulicidae, Siphonaptera) from animal mummies of the Chiribaya Culture, Southern Peru

Dittmar

Mamat

Whiting

et al. 2003

Mem. Inst. Oswaldo Cruz

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“…These therapeutic applications require high amounts of highly pure pDNA. There is a variety of purification strategies available for the purification of pDNA using conventional methods, including tangential flow filtration (TFF) [59][60][61], membrane adsorption [62][63][64] and chromatography [65,66]. Monolithic columns with different functionalities have also been tested for the purification of pDNA.…”

Section: Nucleic Acid Purificationmentioning

confidence: 99%

Monoliths in Bioprocess Technology

2015

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Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a 'yellow pages' for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.

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“…Scalable techniques, like tangential flow ultrafiltration (TFUF) (20), can also be used to desalinate and reduce the volume (6,16). TFUF using a polysulfone membrane with a cut size of 500 kDa and 1000 kDa has been reported to remove more than 90 % of the RNA and 95 % of the protein of clarified lysates, using CaCl 2 (6,12).…”

Section: Introductionmentioning

confidence: 99%