Purification of plasma membranes from dry maize embryos

Sánchez‐Nieto, Sobeida; García‐Rubio, Oscar; Pacheco-Moisés, Fermín Paul; Carballo, Aquiles Carballo; Rodríguez‐Sotres, Rogelio; Gavilanes‐Ruíz, Marina

doi:10.1111/j.1399-3054.1997.tb01832.x

Cited by 14 publications

(6 citation statements)

References 51 publications

(39 reference statements)

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“…S4 ). Therefore it is possible that the low ΔpH values shown by the PMV were due to the natural leakiness of these membrane vesicles, whose molecular architecture is in a reconstruction process because of the recent tissue rehydration at these early times of imbibition ( Powell and Matthews, 1978 ; Sánchez-Nieto et al , 1997 , 1998 ).…”

Section: Resultsmentioning

confidence: 99%

Early carbon mobilization and radicle protrusion in maize germination

Sánchez-Linares

Gavilanes‐Ruíz

Díaz-Pontones

et al. 2012

Journal of Experimental Botany

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Considerable amounts of information is available on the complex carbohydrates that are mobilized and utilized by the seed to support early seedling development. These events occur after radicle has protruded from the seed. However, scarce information is available on the role of the endogenous soluble carbohydrates from the embryo in the first hours of germination. The present work analysed how the soluble carbohydrate reserves in isolated maize embryos are mobilized during 6–24 h of water imbibition, an interval that exclusively embraces the first two phases of the germination process. It was found that sucrose constitutes a very significant reserve in the scutellum and that it is efficiently consumed during the time in which the adjacent embryo axis is engaged in an active metabolism. Sucrose transporter was immunolocalized in the scutellum and in vascular elements. In parallel, a cell-wall invertase activity, which hydrolyses sucrose, developed in the embryo axis, which favoured higher glucose uptake. Sucrose and hexose transporters were active in the embryo tissues, together with the plasma membrane H+-ATPase, which was localized in all embryo regions involved in both nutrient transport and active cell elongation to support radicle extension. It is proposed that, during the initial maize germination phases, a net flow of sucrose takes place from the scutellum towards the embryo axis and regions that undergo elongation. During radicle extension, sucrose and hexose transporters, as well as H+-ATPase, become the fundamental proteins that orchestrate the transport of nutrients required for successful germination.

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Section: Resultsmentioning

confidence: 99%

Early carbon mobilization and radicle protrusion in maize germination

Sánchez-Linares

Gavilanes‐Ruíz

Díaz-Pontones

et al. 2012

Journal of Experimental Botany

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“…Embryos imbibed for 24 h were frozen and homogenized as described previously [16] and then PM were isolated by the procedure in [65] until the obtention of the U 2 fraction. The membrane suspension had an ATPase activity that was 4-fold enriched in glucan synthase II activity (a PM enzyme marker) and was 75%-85% vanadate-sensitive.…”

Section: Isolation Of Pm Vesiclesmentioning

confidence: 99%

Sphingolipid Effects on the Plasma Membrane Produced by Addition of Fumonisin B1 to Maize Embryos

Gutiérrez-Nájera

Saucedo-García

Noyola-Martínez

et al. 2020

Plants

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Fumonisin B1 is a mycotoxin produced by Fusarium verticillioides that modifies the membrane properties from animal cells and inhibits complex sphingolipids synthesis through the inhibition of ceramide synthase. The aim of this work was to determine the effect of Fumonisin B1 on the plant plasma membrane when the mycotoxin was added to germinating maize embryos. Fumonisin B1 addition to the embryos diminished plasma membrane fluidity, increased electrolyte leakage, caused a 7-fold increase of sphinganine and a small decrease in glucosylceramide in the plasma membrane, without affecting phytosphingosine levels or fatty acid composition. A 20%–30% inhibition of the plasma membrane H+-ATPase activity was observed when embryos were germinated in the presence of the mycotoxin. Such inhibition was only associated to the decrease in glucosylceramide and the addition of exogenous ceramide to the embryos relieved the inhibition of Fumonisin B1. These results indicate that exposure of the maize embryos for 24 h to Fumonisin B1 allowed the mycotoxin to target ceramide synthase at the endoplasmic reticulum, eliciting an imbalance of endogenous sphingolipids. The latter disrupted membrane properties and inhibited the plasma membrane H+-ATPase activity. Altogether, these results illustrate the mode of action of the pathogen and a plant defense strategy.

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“…Activities and kinetic analysis from plasma membrane H + -ATPase were performed as previously described [85]. ATP hydrolysis was measured by incubation of the PMV in a reaction medium that included Na3VO4, a specific inhibitor of the plasma membrane H + -ATPase or a mixture of NaN 3 , KNO 3 , and NaMoO 4 , specific inhibitors of mitochondrial ATPase, tonoplast ATPase, and acid phosphatase, respectively.…”

Section: Atpase Activity and Kinetic Assaysmentioning

confidence: 99%

MPK6 Kinase Regulates Plasma Membrane H+-ATPase Activity in Cold Acclimation

Ponce-Pineda

Carmona-Salazar

Saucedo-García

et al. 2021

IJMS

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Cold and freezing stresses severely affect plant growth, development, and survival rate. Some plant species have evolved a process known as cold acclimation, in which plants exposed to temperatures above 0 °C trigger biochemical and physiological changes to survive freezing. During this response, several signaling events are mediated by transducers, such as mitogen activated protein kinase (MAPK) cascades. Plasma membrane H+-ATPase is a key enzyme for the plant cell life under regular and stress conditions. Using wild type and mpk3 and mpk6 knock out mutants in Arabidopsis thaliana, we explored the transcriptional, translational, and 14-3-3 protein regulation of the plasma membrane H+-ATPase activity under the acclimation process. The kinetic analysis revealed a differential profiling of the H+-ATPase activity depending on the presence or absence of MPK3 or MPK6 under non-acclimated or acclimated conditions. Negative regulation of the plasma membrane H+-ATPase activity was found to be exerted by MPK3 in non-acclimated conditions and by MPK6 in acclimated conditions, describing a novel form of regulation of this master ATPase. The MPK6 regulation involved changes in plasma membrane fluidity. Moreover, our results indicated that MPK6 is a critical regulator in the process of cold acclimation that leads to freezing tolerance and further survival.

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Purification of plasma membranes from dry maize embryos

Cited by 14 publications

References 51 publications

Early carbon mobilization and radicle protrusion in maize germination

Early carbon mobilization and radicle protrusion in maize germination

Sphingolipid Effects on the Plasma Membrane Produced by Addition of Fumonisin B1 to Maize Embryos

MPK6 Kinase Regulates Plasma Membrane H+-ATPase Activity in Cold Acclimation

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