Purification of multiple vitellogenins in grey mullet (Mugil cephalus)

Amano, Haruna; Fujita, Toshiaki; Hiramatsu, Naoshi; Sawaguchi, Sayumi; Matsubara, Takahiro; Sullivan, Craig V.; Hara, Akihiko

doi:10.1007/s00227-007-0768-z

Cited by 28 publications

(23 citation statements)

References 29 publications

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“…IgG was purified with ammonium sulfate precipitation and DE52 (Watman International Ltd. Kent, UK) ion-exchange chromatography. It is known that the grey mullet has VTG protein of three forms (A, B, C) (Amano et al, 2007). The used antibody in our experiment indicated a positive reaction to complete type of VTG-A and -B, which is the main VTG protein induced by estrogens.…”

Section: Measurement Of Vtg Levels In Serumsupporting

confidence: 47%

Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet (Mugil cephalus)

Aoki

Nagae

Takao

et al. 2010

Science of The Total Environment

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We monitored the contamination by environmental estrogens (EEs) of coastal areas in Korea and Japan using the wild grey mullet. The grey mullet were collected from Ansan, Jeju, Yeosu, Tongyeong, and Busan in Korea and Nagasaki, Omuta, and Fukuoka in Japan.Contamination by EEs was determined by measuring vitellogenin (VTG) levels in serum and identifying gonadal abnormalities histologically (i.e., testis-ova). In four sites in Korea (Ansan, Yeosu, Tongyeong, and Busan) and two sites in Japan (Nagasaki and Fukuoka), serum VTG in immature and male grey mullet was detected at levels greater than 1.0 g/ml, which is considered to be an abnormal level. Although, testis-ova were observed in some individuals collected in Ansan, Tongyeong, and Busan in Korea and Omuta in Japan, there was no correlation between individuals with testis-ova and individuals with abnormal levels of VTG. Furthermore, in Japan, serum VTG levels of fish collected from Nagasaki and Fukuoka was also greater than 1.0 g/ml. Although individuals with testis-ova were found in Omuta, these fish expressed normal levels of serum VTG. Our results suggest that the grey mullet living in these coastal areas are influenced by EEs in the environment. Furthermore, it appears that the production of VTG and the occurrence of testis-ova are caused by different mechanisms.

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Section: Measurement Of Vtg Levels In Serumsupporting

confidence: 47%

Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet (Mugil cephalus)

Aoki

Nagae

Takao

et al. 2010

Science of The Total Environment

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“…were smaller than those of the complete-type Vg (~540 kDa and ~165 kDa, respectively; Hiramatsu and Hara, 1996), which is consistent with results commonly found for other teleosts, 2) the masses of taimen VgC were similar to those reported for the VgC of other teleosts (Hiramatsu et al, 2002b;Shimizu et al, 2002;Amano et al, 2007b), 3) taimen…”

Section: Discussionsupporting

confidence: 89%

Purification and characterization of a novel incomplete-type vitellogenin protein (VgC) in Sakhalin taimen (Hucho perryi)

Amano

Mochizuki

Fujita

et al. 2010

Comparative Biochemistry and Physiology Part A: Molecular &

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A novel, incomplete-type vitellogenin (VgC) and its derived yolk lipovitellin (LvC) were immunologically detected in female serum and egg extracts, respectively, of Sakhalin taimen (Hucho perryi) using a subtype-specific antiserum against LvC of grey mullet (Mugil cephalus). The taimen VgC was purified from the sera of vitellogenic females by a combination of gel filtration, anion exchange, and immunoadsorbent column chromatography. Gel filtration of the purified VgC revealed that it had an apparent native mass of ~380 kDa, while the mass of the VgC polypeptide that appeared following SDS-PAGE was estimated to be ~140 kDa. An antiserum was raised against the purified VgC and utilized for the development of a subtype-specific immunoassay for VgC. Levels of VgC in the serum of female taimen increased from 25 µg/ml to ~1 mg/ml, with an increase of GSI. Levels of complete-type Vg and estradiol-17β (E 2 ) in the serum of E 2 -administered juvenile taimen increased and reached peak levels similar to those found in vitellogenic females. Although VgC could be induced in the serum of E 2 -administered taimen, it stayed at levels (35.5-73 µg/ml) lower than those obtained in females. This is the first report on the presence of serum VgC and yolk LvC in a salmonid species; these findings indicate that for Sakhalin taimen, like other highly-evolved teleost species, this minor subtype of Vg is significant in the formation of egg yolk.

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“…To date, the complete Vtg (VtgA and/or VtgB) and incomplete Vtg (VtgC) have been successfully isolated and purified as two different proteins with distinct molecular weights and antigenicity in various fish species, including tilapia (O. aureus [85] and O. mossambicus [86]), medaka [87], Japanese goby Acanthogobius flavimanus [88] and Sakhalin taimen [89]. In addition, three distinct types of Vtg have been purified or detected in mosquitofish Gambusia affinis [90], red seabream Pagrus major [91] and grey mullet [92]. Two or three types of Vtgs have been detected as proteins with distinct antigenicity in various existing fish species [66].…”

Section: Multiplicity Of Fish Vitellogeninsmentioning

confidence: 99%