Purification of membrane fragments derived from the non excitable surface of the eel electroplax

Bauman, Annie; Changeux, Jean‐Pierre; Benda, P; Huchet, Monique

doi:10.1016/0014-5793(70)80248-4

Cited by 36 publications

(7 citation statements)

References 10 publications

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“…Most postsynaptic membrane preparations from Torpedo contain significant amounts of a 90K protein. Some evidence suggests that this component is a subunit of the Na+,K+-ATPase (7, 16), a protein found in high concentrations in the non-innervated membrane of electrocytes (3) . Our finding that mabs to a 90K protein react intensely with the noninnervated membrane is consistent with this proposal .…”

Section: Discussionmentioning

confidence: 99%

Peripheral proteins of postsynaptic membranes from Torpedo electric organ identified with monoclonal antibodies.

Froehner¹

1984

The Journal of Cell Biology

100

101

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Highly purified postsynaptic membranes from Torpedo electric organ contain the acetylcholine receptor as well as other proteins . To identify synapse-specific components, we prepared monoclonal antibodies (mabs) to proteins extracted from the membranes with either lithium diiodosalicylate or alkaline treatment . 10 mabs specific for three different proteins were obtained . Seven mabs reacted with a major 43,000-mol-wt protein (43K protein) . This protein is composed of isoelectric variants (pl = 7.2-7.8) and each of the mabs reacted with all of the variants. Analysis of these mabs by competition for binding to 43K protein and by reaction with proteolytic fragments of 43K protein in immunoblots showed that they recognize at least five different epitopes. Two mabs reacted with a protein of 90,000 mol wt (90K protein) and one with a protein of 58,000 mol wt composed of isoelectric variants (pl = 6.4-6.7) (58K protein) . The 43K and 58K proteins appeared to co-purify with the receptorcontaining membranes while the 90K protein did not . Immunofluorescence experiments indicated that the anti-43K mabs bind to the innervated face of Torpedo electrocytes and that a component related to the 43K protein is found at the rat neuromuscular junction . The anti-58K mab stained the innervated face, although rather weakly, while the anti-90K mabs reacted intensely with the non-innervated membrane . Thus, the 43K protein and possibly also the 58K protein are synaptic components while the 90K protein is predominantly nonsynaptic .

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Section: Discussionmentioning

confidence: 99%

Peripheral proteins of postsynaptic membranes from Torpedo electric organ identified with monoclonal antibodies.

Froehner¹

1984

The Journal of Cell Biology

100

101

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“…This has been successful from the eel electroplax [1] and the lobster nerve fibers [2,3]. From brain tissue, isolation of plasma membranes has met with serious difficulties due to the abundance of non excitable membranes present.…”

Section: Introductionmentioning

confidence: 99%

Purification of plasma membranes from immature brain

Hemminki

1973

FEBS Letters

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“…The reader is referred, for example, to work from Salton's laboratory on the cell membrane of Micrococcus lysodeikticus (Mufioz et al, 1968(Mufioz et al, a, 1968(Mufioz et al, b, 1969 and to the work of Changeux and his co-workers on the membranes of the electroplax (Changeux et al, 1969;Bauman et al, 1970).…”

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confidence: 99%