Purification of Membrane-bound Lactoferrin from the Human Milk Fat Globule Membrane

Cho, Jin-Kook; Azuma, Norihiro; Lee, Chi H.; Yu, Jae-Hyeun; Kanno, Choemon

doi:10.1271/bbb.64.633

Cited by 9 publications

(6 citation statements)

References 13 publications

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“…LTF was relatively abundant in the MFGM fraction of human milk (Table 3), which may seem remarkable for a secreted protein. A previous study, however, found that part of the LTF in human milk was strongly bound to the MFGM membrane [23]. This finding may be related to the defense of the epithelial membrane of the mammary gland, as MFGM originates from the epithelial membrane.…”

Section: Resultsmentioning

confidence: 82%

The Host Defense Proteome of Human and Bovine Milk

et al. 2011

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Milk is the single source of nutrients for the newborn mammal. The composition of milk of different mammals has been adapted during evolution of the species to fulfill the needs of the offspring. Milk not only provides nutrients, but it also serves as a medium for transfer of host defense components to the offspring. The host defense proteins in the milk of different mammalian species are expected to reveal signatures of evolution. The aim of this study is therefore to study the difference in the host defense proteome of human and bovine milk. We analyzed human and bovine milk using a shot-gun proteomics approach focusing on host defense-related proteins. In total, 268 proteins in human milk and 269 proteins in bovine milk were identified. Of these, 44 from human milk and 51 from bovine milk are related to the host defense system. Of these proteins, 33 were found in both species but with significantly different quantities. High concentrations of proteins involved in the mucosal immune system, immunoglobulin A, CD14, lactoferrin, and lysozyme, were present in human milk. The human newborn is known to be deficient for at least two of these proteins (immunoglobulin A and CD14). On the other hand, antimicrobial proteins (5 cathelicidins and lactoperoxidase) were abundant in bovine milk. The high concentration of lactoperoxidase is probably linked to the high amount of thiocyanate in the plant-based diet of cows. This first detailed analysis of host defense proteins in human and bovine milk is an important step in understanding the function of milk in the development of the immune system of these two mammals.

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Section: Resultsmentioning

confidence: 82%

The Host Defense Proteome of Human and Bovine Milk

et al. 2011

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“…The bioactive peptides, products of casein digestion, for example, have been linked to numerous functions, such gastrointestinal and immunological function, infant development, and antibiotic and probiotic functionality (Park and Nam 2015) (Table 3). Although lactoferrin is found in the aqueous phase of milk as a whey protein, it is also relatively abundant in the human MFGM fraction and was reported to be bound to the MFGM membrane (Cho et al 2000) for new-born host defence functionality. Although those could easily be harvested from skim milk, the presence in the enriched MFGM extract could contribute to the functionality of MFGs (Luo et al 2014), and are outlined in Table 3.…”

Section: Milk Fat Globule Bioactive Proteinsmentioning

confidence: 99%

Variation in milk fat globule size and composition: A source of bioactives for human health

Thum

Roy

Everett

et al. 2021

Critical Reviews in Food Science and Nutrition

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Milk fat globules (MFGs) are secreted from the mammalian gland and are composed of a triacylglycerol core surrounded by a triple membrane structure, the milk fat globule membrane (MFGM). The MFGM contains complex lipids and proteins reported to have nutritional, immunological, neurological and digestive functions. Human and ruminant milk are shown to share a similar MFG structure but with different size, profile and abundance of protein and polar lipids. This review summarizes the reported data on human, bovine, caprine and ovine MFG composition and concentration of bioactive components in different MFG-size fractions. A comprehensive understanding of compositional variations between milk from different species and MFG size fractions may help promote various milk sources as targeted supplements to improve human development and health. MFG size and MFGM composition are species-specific and affected by lactation, diet and breed (or maternal origin). Purification and enrichment methods for some bioactive proteins and lipids present in the MFGM have yet to be established or are not scaled sufficiently to be used to supplement human diets. To overcome this problem, MFG size selection through fractionation or herd selection may provide a convenient way to pre-enrich the MFG fraction with specific protein and lipid components to fulfill human dietary and health requirements.

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“…This experimental procedure diminished the potential interaction of Lac with Con A. Furthermore, although the abilities of human and bovine Lac in binding to Con A are similar (28, 30, 31), our data demonstrated that only bovine Lac, but not human Lac, could protect mice against Con A‐induced hepatitis (data not shown). This finding again indicates that the protective function of Lac is not because of a direct binding to Con A.…”

Section: Discussionmentioning

confidence: 72%