Purification of lumbrokinase from Eisenia fetida using aqueous two-phase systems and anion-exchange chromatography

Iannucci, Nancy B.; Camperi, Silvia A.; Cascone, Osvaldo

doi:10.1016/j.seppur.2008.08.014

Cited by 15 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neves et al [42] obtained a yield of 115% using PEG with a molar mass of 8000 g/mol and citrate concentration of 12% in the production of phytase by Absidia blakesleeana URM5604. In the case of the purification of lumbrokinase, a fibrinolytic enzyme from earthworm Eisenia fetida, using a PEG/phosphate system, Iannucci et al [43] reported a yield of 99%, and a purification factor of 1.0, values that are lower than the ones obtained in our work.…”

Section: Fibrinolytic Protease Partition Yield and Purification Factorcontrasting

confidence: 88%

Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems

Silva

Marques

Porto

et al. 2013

Fluid Phase Equilibria

View full text Add to dashboard Cite

Extraction of fibrinolytic protease from Streptomyces sp. DPUA1576 fermentation broth was performed using polyethylene glycol (PEG)-phosphate salts aqueous two phase system (ATPS). The influence of pH, PEG molar mass, PEG and phosphate concentrations on fibrinolytic protease partition coefficient (K) were evaluated by 2 4 full factorial design. Fibrinolytic protease partitioned preferentially to the (PEG)rich phase with the highest partition coefficient (K = 37.00) obtained in the system 20% (w/w) PEG 1500 (g/mol)-14% (w/w) phosphate at pH 8.0. The system that allowed for the highest extraction consisted of 15% (w/w) PEG 3350-12% (w/w) phosphate, at pH 7.0. In these conditions, a purification factor of 1.51 was obtained for a partition coefficient of 6.41 with the fibrinolytic activity (FA) retained in the top phase. Obtained results confirm the interest of considering ATPS as an alternative extraction method for fibrinolytic protease from Streptomyces sp. DPUA1576.

show abstract

Section: Fibrinolytic Protease Partition Yield and Purification Factorcontrasting

confidence: 88%

Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems

Silva

Marques

Porto

et al. 2013

Fluid Phase Equilibria

View full text Add to dashboard Cite

show abstract

“…In the last decades, many fibrinolytic enzymes from natural resources, such as snakes [18], earthworms [19,20], insects [21], plants [22], mushrooms [23], microorganisms [24,25] and fermented foods such as Chungkook-jang [26] and Tempeh [27], have been identified and studied. Even though these enzymes have been characterized from a wide range of different sources, microbial fibrinolytic enzymes are considered attractive tools due to their features, such as enhanced specificity [8], low production cost [8], comparatively high yield [28] and the possibility to be genetically modified by recombinant DNA technology and protein engineering approaches [29].…”

Section: Introductionmentioning

confidence: 99%

Marine Microbial Fibrinolytic Enzymes: An Overview of Source, Production, Biochemical Properties and Thrombolytic Activity

2022

View full text Add to dashboard Cite

Cardiovascular diseases (CVDs) have emerged as a major threat to global health resulting in a decrease in life expectancy with respect to humans. Thrombosis is one of the foremost causes of CVDs, and it is characterized by the unwanted formation of fibrin clots. Recently, microbial fibrinolytic enzymes due to their specific features have gained much more attention than conventional thrombolytic agents for the treatment of thrombosis. Marine microorganisms including bacteria and microalgae have the significant ability to produce fibrinolytic enzymes with improved pharmacological properties and lesser side effects and, hence, are considered as prospective candidates for large scale production of these enzymes. The current review presents an outline regarding isolation sources, production, features, and thrombolytic potential of fibrinolytic biocatalysts from marine microorganisms identified so far.

show abstract

“…Lumbrokinase is a fibrinolytic serine protease enzyme with potent fibrinolytic activity and inhibitory effects on platelet aggregation [119]. Lumbrokinase was first extracted from the earthworm L. rubellus [120] and later from other earthworm species such as Eisenia fetida [121]. Here, we report for the first-time lumbrokinase from leeches.…”

Section: Genetic Variation Of Antithrombotic Proteinsmentioning

confidence: 90%

Revisiting the Asian Buffalo Leech (Hirudinaria manillensis) Genome: Focus on Antithrombotic Genes and Their Corresponding Proteins

Liu,

Zhao,

Huang

et al. 2023

Genes

View full text Add to dashboard Cite

Leeches are well-known annelids due to their obligate blood-feeding habits. Some leech species secrete various biologically active substances which have important medical and pharmaceutical value in antithrombotic treatments. In this study, we provided a high-quality genome of the Asian buffalo leech (Hirudinaria manillensis), based on which we performed a systematic identification of potential antithrombotic genes and their corresponding proteins. Combining automatic and manual prediction, we identified 21 antithrombotic gene families including fourteen coagulation inhibitors, three platelet aggregation inhibitors, three fibrinolysis enhancers, and one tissue penetration enhancer. A total of 72 antithrombotic genes, including two pseudogenes, were identified, including most of their corresponding proteins forming three or more disulfide bonds. Three protein families (LDTI, antistasin, and granulin) had internal tandem repeats containing 6, 10, and 12 conserved cysteines, respectively. We also measured the anticoagulant activities of the five identified hirudins (hirudin_Hman1 ~ hirudin_Hman5). The results showed that three (hirudin_Hman1, hirudin_Hman2, and hirudin_Hman5), but not the remaining two, exhibited anticoagulant activities. Our study provides the most comprehensive collection of antithrombotic biomacromolecules from a leech to date. These results will greatly facilitate the research and application of leech derivatives for medical and pharmaceutical purposes in the treatment of thrombotic diseases.

show abstract

Purification of lumbrokinase from Eisenia fetida using aqueous two-phase systems and anion-exchange chromatography

Cited by 15 publications

References 9 publications

Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems

Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems

Marine Microbial Fibrinolytic Enzymes: An Overview of Source, Production, Biochemical Properties and Thrombolytic Activity

Revisiting the Asian Buffalo Leech (Hirudinaria manillensis) Genome: Focus on Antithrombotic Genes and Their Corresponding Proteins

Contact Info

Product

Resources

About