Purification of Lectin from Larvae of the fly,<i>Musca domestica</i>, and in Vitro Anti-Tumor Activity in MCF-7 Cells

Cao, Xiwang; Huo, Zhen; Lu, Ming–Wei; Mao, Dezhi; Zhao, Qiuyan; Xu, Cheng‐Jian; Wang, C.; Zeng, Bin

doi:10.1673/031.010.14124

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…As galactose could inhibit the activity of the lectin molecule, it was used to couple Sepharose 6B and used as the affinity matrix for purification of lectin from the serum of P. lepida. Sepharose has been extensively used as an adsorbent for the isolation of lectins specific to galactosides and related Cao et al 2010;Kato et al 2011;Pohleven et al 2011). Lectin isolated from P. lepida was relatively similar to that of the lectin isolated from R. prolixus, in that they both agglutinate rabbit RBC in a calciumindependent manner and have galactose specificity (Ratcliffe et al 1996), although confirmation awaits future molecular characterization.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Arumugam

Hilda

Bhuvaragavan

et al. 2019

Entomological Science

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Insects depend on lectins for non‐self recognition and clearance of invading pathogens. Naturally occurring lectin showing specificity for galactose was purified from the serum of lepidopteran pest Parasa lepida by affinity chromatography using Sepharose 6B coupled with galactose as a gel matrix. Preliminary studies on crude serum agglutinin revealed that the agglutinin molecule showed varying degrees of specificity to avian and mammalian red blood cells tested. Among them, the highest titer of 128 was recorded against rabbit red blood cell type. The agglutinin molecule in the crude serum was stable up to 60°C and at pH between 6 and 9. Also, the hemagglutinating activity was neither dependent on divalent cations nor sensitive to ethylenediaminetetraacetic acid treatment. Galactose inhibited the hemagglutinating activity at minimum inhibitory concentration of 12.5 mM and hence it was used as a ligand for affinity chromatography. Native polyacrylamide gel electrophoresis analysis revealed a single band and the molecular weight of the lectin was found to be approximately 90 kDa. Bacterial agglutination activity of the purified lectin with two significant toxin bacteria, namely Salmonella typhi and Bacillus thuringiensis, was observed.

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Section: Discussionmentioning

confidence: 99%

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Arumugam

Hilda

Bhuvaragavan

et al. 2019

Entomological Science

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“…The effect of SLCAs on [Ca 2+ ]i release was examined quantitatively and qualitatively using Fluo 4-acetoxymethyl ester (Fluo-4 AM) dye as described previously [ 15 ]. Briefly, HeLa 1x10 5 ml -1 cells were treated with sophorolipids for 4, 8 and 12 h. Following incubation, the cells were washed with PBS and loaded with Fluo-4 AM (4 μM).…”

Section: Methodsmentioning

confidence: 99%

Anti-proliferative effect of novel primary cetyl alcohol derived sophorolipids against human cervical cancer cells HeLa

et al. 2017

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Sophorolipids (SLs) are glycolipid biosurfactants that have been shown to display anticancer activity. In the present study, we report anti-proliferative studies on purified forms of novel SLs synthesized using cetyl alcohol as the substrate (referred as SLCA) and their anticancer mechanism in human cervical cancer cells. Antiproliferative effect of column purified SLCA fractions (A, B, C, D, E and F) was examined in panel of human cancer cell lines as well as primary cells. Among these fractions, SLCA B and C significantly inhibited the survival of HeLa and HCT 116 cells without affecting the viability of normal human umbilical vein endothelial cells (HUVEC). The two fractions were identified as cetyl alcohol sophorolipids with non-hydroxylated tail differing in the degree of acetylation on sophorose head group. At an IC50 concentration SLCA B (16.32 μg ml-1) and SLCA C (14.14 μg ml-1) blocked the cell cycle progression of HeLa cells at G1/S phase in time-dependent manner. Moreover, SLCA B and SLCA C induced apoptosis in HeLa cells through an increase in intracellular Ca2+ leading to depolarization of mitochondrial membrane potential and increase in the caspase-3, -8 and -9 activity. All these findings suggest that these SLCAs could be explored for their chemopreventive potential in cervical cancer.

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“…The cells were kept in the dark for 20 min. The cells were analyzed using a FACSort flow cytometer [33]. …”

Section: Methodsmentioning

confidence: 99%

A Hemagglutinin from Northeast Red Beans with Immunomodulatory Activity and Anti-proliferative and Apoptosis-inducing Activities Toward Tumor Cells

Chan¹,

Wong²,

Fang³

et al. 2013

PPL

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A 64-kDa hemagglutinin from a Phaseolus vulgaris cultivar, the northeast red bean, was purified by a protocol composed of three chromatographic steps involving affinity chromatography on Affi-gel blue gel, cation exchange chromatography on SP-Sepharose and FPLC-gel filtration on Superdex 75. The purified hemagglutinin appeared as a single 32-kDa band in SDS-PAGE indicating its dimeric nature. The N-terminal amino acid sequence of the hemagglutinin resembled the sequences of lectins and hemagglutinins from a number of Phaseolus species. The hemagglutinin manifested moderate thermostability and pH stability. It retained full activity up to 65 °C and in the pH range 2 – 12. It did not interact with simple sugars such as glucose, mannose and galactose. The hemagglutinin exerted immunostimulatory effects by upregulating the expression of cytokines like interferon-γ and tumor necrosis factor-α. It also exhibited antiproliferative activity on a number of tumor cells including MCF7 (breast cancer), HepG2 (liver cancer), CNE1 and CNE2 (nasopharyngeal cancer) cells, with stronger activity toward MCF7 and CNE1 cells. The hemagglutinin induced phophatidylserine externalization, mitochondrial depolarization and DNA condensation in MCF7 cells, indicating initiation of apoptosis. However, at high hemagglutinin concentrations, severe damage to the MCF7 cells was detected.

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Purification of Lectin from Larvae of the fly,Musca domestica, and in Vitro Anti-Tumor Activity in MCF-7 Cells

Cited by 8 publications

References 34 publications

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Anti-proliferative effect of novel primary cetyl alcohol derived sophorolipids against human cervical cancer cells HeLa

A Hemagglutinin from Northeast Red Beans with Immunomodulatory Activity and Anti-proliferative and Apoptosis-inducing Activities Toward Tumor Cells

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