2010
DOI: 10.1673/031.010.14124
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Purification of Lectin from Larvae of the fly,Musca domestica, and in Vitro Anti-Tumor Activity in MCF-7 Cells

Abstract: A new lectin was purified from larvae of the fly, Musca domestica L. (Diptera: Muscidae) (MLL-2, 38 kDa) using affinity chromatography and HPLC. Anti-tumor activity of MLL-2 was demonstrated by its inhibition of proliferation of human breast cancer (MCF-7) cells in a time-and dose-dependent manner. The results of acridine orange staining indicated that MLL-2 caused apoptosis in MCF-7 cells. DNA fragmentation in MCF-7 cells has been detected by TUNEL. Flow cytometric analysis also demonstrated that MLL-2 caused… Show more

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Cited by 8 publications
(7 citation statements)
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“…As galactose could inhibit the activity of the lectin molecule, it was used to couple Sepharose 6B and used as the affinity matrix for purification of lectin from the serum of P. lepida. Sepharose has been extensively used as an adsorbent for the isolation of lectins specific to galactosides and related Cao et al 2010;Kato et al 2011;Pohleven et al 2011). Lectin isolated from P. lepida was relatively similar to that of the lectin isolated from R. prolixus, in that they both agglutinate rabbit RBC in a calciumindependent manner and have galactose specificity (Ratcliffe et al 1996), although confirmation awaits future molecular characterization.…”
Section: Discussionmentioning
confidence: 99%
“…As galactose could inhibit the activity of the lectin molecule, it was used to couple Sepharose 6B and used as the affinity matrix for purification of lectin from the serum of P. lepida. Sepharose has been extensively used as an adsorbent for the isolation of lectins specific to galactosides and related Cao et al 2010;Kato et al 2011;Pohleven et al 2011). Lectin isolated from P. lepida was relatively similar to that of the lectin isolated from R. prolixus, in that they both agglutinate rabbit RBC in a calciumindependent manner and have galactose specificity (Ratcliffe et al 1996), although confirmation awaits future molecular characterization.…”
Section: Discussionmentioning
confidence: 99%
“…The effect of SLCAs on [Ca 2+ ]i release was examined quantitatively and qualitatively using Fluo 4-acetoxymethyl ester (Fluo-4 AM) dye as described previously [ 15 ]. Briefly, HeLa 1x10 5 ml -1 cells were treated with sophorolipids for 4, 8 and 12 h. Following incubation, the cells were washed with PBS and loaded with Fluo-4 AM (4 μM).…”
Section: Methodsmentioning
confidence: 99%
“…The cells were kept in the dark for 20 min. The cells were analyzed using a FACSort flow cytometer [33]. …”
Section: Methodsmentioning
confidence: 99%