Purification of infective baculoviruses by monoliths

Gerster, Petra; Kopecky, Eva-Maria; Hammerschmidt, Nikolaus; Klausberger, Miriam; Krammer, Florian; Grabherr, Reingard; Mersich, Christa; Urbas, Lidija; Kramberger, Petra; Paril, Tina; Schreiner, Matthias; Nöbauer, Katharina; Razzazi-Fazeli, Ebrahim; Jungbauer, Alois

doi:10.1016/j.chroma.2013.03.047

Cited by 36 publications

(19 citation statements)

References 27 publications

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“…On the other hand, monoliths display a higher accessible surface area for large molecules and hence greater binding capacity [6,7]. Among the large molecules of interest viruses [8][9][10], virus-like particles [11], nucleic acids, and plasmid DNA [12][13][14] have been successfully isolated and purified by several research groups demonstrating the advantages of monolithic supports for these applications.…”

Section: Introductionmentioning

confidence: 99%

Experimental characterization of the transport phenomena, adsorption, and elution in a protein A affinity monolithic medium

Herigstad

Dimartino

Boi

et al. 2015

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

Experimental characterization of the transport phenomena, adsorption, and elution in a protein A affinity monolithic medium

Herigstad

Dimartino

Boi

et al. 2015

Journal of Chromatography A

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“…Using a pre-column step has been shown to reduce interfering compounds and substances influencing the capacity of anion exchange monoliths previously [43]. Therefore, we decided to use this strategy by including a CIM OH monolithic pre-column in order to remove such compounds from the effluent and to assess the effect on the pressure build-up observed in the subsequent CIM QA monolithic column.…”

Section: Solving Back Pressure Issuementioning

confidence: 99%

Methacrylate monolith chromatography as a tool for waterborne virus removal

Rački

Kramberger

Steyer

et al. 2015

Journal of Chromatography A

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Enteric viruses are commonly present in environmental waters and represent the major cause of waterborne infections and outbreaks. Since traditional wastewater treatments fail to remove enteric viruses in the water purification process, they are released daily into environmental waters. Monolithic supports have enabled chromatography to enter the field of virology. They have been successfully used in virus purification and concentration. In this work quaternary amine (QA) methacrylate monoliths were exploited to remove enteric viruses from wastewater treatment plant effluent. Expectedly, chromatographic processing of such a complex medium was troublesome, even for monoliths, characterized by extremely large pore dimensions. This problem was solved by introducing a pre-step chromatography using hydroxyl (OH) methacrylate monoliths. This way, molecules, that would hinder virus binding to the anion-exchanger monolith, were removed. As a result, the OH pre-column reduced backpressure increase on the subsequent anion-exchanger column, and increased both QA column binding capacity and life time. Wastewater effluent samples were successfully purified from five waterborne enteric viruses (rotavirus, norovirus genogroup I and II, astrovirus, sapovirus), below the detection limit of RT-qPCR. The breakthrough of the rotavirus binding capacity was not reached for concentrations that significantly exceeded those expected in effluent waters. The obtained results confirm that methacrylate monoliths can be a valuable tool for simultaneous removal of different waterborne viruses from contaminated water sources.

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“…In another study, baculovirus from cell culture supernatant was successfully purified using the anion exchange monolithic column CIM ® QA [30]. The authors were able to reduce the volume 82-fold, increase activity 51-fold and remove 99% of the contaminants.…”

Section: Virus Purificationmentioning

confidence: 99%

“…Due to the high flow velocity and low pressure drop, the overall process time using monoliths is much lower compared to particle-based resins [30]. For example, Rupar et al were able to purify potato virus Y with the help of monolithic columns in half the separation time in comparison to classical methods.…”

Section: Separation Timementioning

confidence: 99%

Monoliths in Bioprocess Technology

2015

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Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a 'yellow pages' for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.

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Purification of infective baculoviruses by monoliths

Cited by 36 publications

References 27 publications

Experimental characterization of the transport phenomena, adsorption, and elution in a protein A affinity monolithic medium

Experimental characterization of the transport phenomena, adsorption, and elution in a protein A affinity monolithic medium

Methacrylate monolith chromatography as a tool for waterborne virus removal

Monoliths in Bioprocess Technology

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