Purification of <i>Lactobacillus acidophilus</i> surface‐layer protein and its immunomodulatory effects on <scp>RAW264</scp>.7 cells

Zhang, Dandan; Wu, Mengting; Guo, Yuxing; Xun, Mingyue; Wang, Wenwen; Wu, Zhen; Pan, Daodong

doi:10.1002/jsfa.8294

Cited by 17 publications

(9 citation statements)

References 24 publications

(31 reference statements)

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“…SLPs of probiotics also have many benefits to the host. Recent studies found that SLPs purified from Lactobacillus exerted immunomodulatory effects, which attenuated intestinal barrier dysfunction and inflammation, and protected intestinal epithelial barrier (Prado Acosta et al, 2016;Zhang et al, 2017;Wang et al, 2019). Surface-layer protein A (SlpA) is a S-layer protein specifically found in L. acidophilus NCFM.…”

Section: Surface-layers Proteinsmentioning

confidence: 99%

Probiotic Gastrointestinal Transit and Colonization After Oral Administration: A Long Journey

Han

Xie

et al. 2021

Front. Cell. Infect. Microbiol.

195

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Orally administered probiotics encounter various challenges on their journey through the mouth, stomach, intestine and colon. The health benefits of probiotics are diminished mainly due to the substantial reduction of viable probiotic bacteria under the harsh conditions in the gastrointestinal tract and the colonization resistance caused by commensal bacteria. In this review, we illustrate the factors affecting probiotic viability and their mucoadhesive properties through their journey in the gastrointestinal tract, including a discussion on various mucosadhesion-related proteins on the probiotic cell surface which facilitate colonization.

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Section: Surface-layers Proteinsmentioning

confidence: 99%

Probiotic Gastrointestinal Transit and Colonization After Oral Administration: A Long Journey

Han

Xie

et al. 2021

Front. Cell. Infect. Microbiol.

195

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“…Our group has previously determined L. acidophilus CICC 6074 S-layer protein as an active ingredient of LAB and tested itscapabilities to regulate the immune system. ,, This research further investigated the underlying molecular mechanism of the S-layer protein on the cytotoxic function against colon cancer HT-29 cells.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies indicated that the L. acidophilus CICC 6074 S-layer protein has intestinal adhesion and immune system regulation abilities. ,, As immunotherapy is considered as an underlying cancer treatment, we predict that the S-layer protein may have potential cytotoxic functions on colon cancer cells.…”

Section: Introductionmentioning

confidence: 93%

“…11 Our previous studies indicated that the L. acidophilus CICC 6074 S-layer protein has intestinal adhesion and immune system regulation abilities. 10,12,13 As immunotherapy is considered as an underlying cancer treatment, we predict that the S-layer protein may have potential cytotoxic functions on colon cancer cells. The purpose of the current research is to explore the effects and the underlying mechanisms of the L. acidophilus CICC 6074 S-layer protein against colon cancer cells.…”

Section: ■ Introductionmentioning

confidence: 99%

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Effect of Lactobacillus acidophilus CICC 6074 S-Layer Protein on Colon Cancer HT-29 Cell Proliferation and Apoptosis

Zhang

Pan

Yang

et al. 2020

J. Agric. Food Chem.

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The objective of this research was to investigate the effects of Lactobacillus acidophilus CICC 6074 S-layer protein on the viability, adhesion, cell cycle, and apoptosis of human colon cancer HT-29 cells and to explore their molecular mechanism of tumor suppression. The S-layer protein at doses of 0, 25, 50, and 100 mg/L significantly suppressed the proliferation of HT-29 cells. The S-layer protein exerts its cytotoxic activities against colon cancer HT-29 cells by arresting the cell cycle in the G1 phase through upregulating the expression of p53, p21, and p16 and downregulating the expression of CDK1 (cyclin-dependent kinases) and cyclin B. Morphological changes were further observed by transmission electron microscopy, and the cells treated with the S-layer protein showed obvious characteristic changes of apoptosis including chromatin condensation, nuclear fragmentation, vacuoles, and so on. Furthermore, our mechanism studies indicated that the S-layer protein may induce HT-29 cell apoptosis through the death receptor apoptotic pathway and mitochondrial pathway and impede cell invasion by inhibiting the synthesis of the PI3K/AKT pathway and FasL. These results demonstrated that the L. acidophilus CICC 6074 S-layer protein may be a potential anticarcinogenic agent.

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“…Gel filtration chromatography can separate small and large molecules from interfering matrices (Lian et al 2016 ). So, it is easy to isolate contaminants from high molecular weight interferences and widely used on the basis of the different molecular weights of proteins employed to separate the different components of a sample (Zhang et al 2017 ). The method can effectively purify the target protein, ensure the biological activity of the recombinant protein, and avoid the irreversible degeneration caused by too-long treatment of the denaturant.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression and adhesion analysis of silent slpB of Lactobacillus acidophilus NCFM

Guo

Yang

et al. 2018

AMB Expr

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The slpB gene of Lactobacillus acidophilus NCFM, which differs from the slpA gene and is silent under normal conditions, was successfully amplified and ligated to the corresponding available sites on a recombinant pET-28a vector. Then the pET-28a-slpB vector was transformed into Escherichia coli DH (DE3) and the fusion His-slpB protein was expressed by induction with 1 mM IPTG for 14 h at 37 °C. The resulting His-slpB protein (SB) had a relative molecular weight of 48 kDa. It was purified using a Ni-NTA column and was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot contrastive analysis. The slpA protein (SA) from L. acidophilus NCFM was extracted and purified. It had a relative molecular weight of 46 kDa. Circular dichroism measurements suggested that the two S-layer proteins had a high β-sheet content and a low α-helix structure content. In an adhesion experiment, SA displayed higher adhesive capability towards Caco-2 cells than did SB. The results suggest that these two S-layer proteins could have biotechnological applications.

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Purification of Lactobacillus acidophilus surface‐layer protein and its immunomodulatory effects on RAW264.7 cells

Abstract: SLP can trigger immunomodulatory effects in RAW 264.7 cells. This provides crucial information that will enable the further use of L. acidophilus in food, medicine and other products. © 2017 Society of Chemical Industry.

Cited by 17 publications

References 24 publications

Probiotic Gastrointestinal Transit and Colonization After Oral Administration: A Long Journey

Probiotic Gastrointestinal Transit and Colonization After Oral Administration: A Long Journey

Effect of Lactobacillus acidophilus CICC 6074 S-Layer Protein on Colon Cancer HT-29 Cell Proliferation and Apoptosis

Molecular cloning, expression and adhesion analysis of silent slpB of Lactobacillus acidophilus NCFM

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