Purification of human leucocyte pyruvate kinase

Fecal pyruvate kinase (M2-PK): A new predictor for inflammation and severity of pouchitis

“…Pyruvate kinase is a ubiquitous enzyme present in virtually all prokaryotic and eukaryotic cells catalyzing the regulatory step in the glycolytic pathway, the conversion of phosphoenolpyruvate (PEP) to pyruvate. Pyruvate kinase exists in two different isotypes: its tetrameric form (M1) has been found in skeletal muscles, heart and brain, whereas the dimeric form (M2) is present in undifferentiated and proliferating tissues [2]. Increased TuM2-PK concentration in polymorphonuclear neutrophils was documented in patients with polytrauma [3] and chronic cardiac failure [4].…”

Comparative Study of Human M2-Type Pyruvate Kinases Isolated from Human Leukocytes and Erythrocytes of a Patient with Red Cell Pyruvate Kinase Hyperactivity

“…We have previously de scribed the preparation of rabbit antisera against pu rified human M2-PK isozyme [22] and against puri fied human R-PK [17].…”

“…Immunoneutralization was performed according to the method previously described [22], Double im munodiffusion was performed according to the method of Ouchterlony [24].…”

“…The pellet was dissolved in a small amount of buffer of the following step and dialysed. The following steps of the purification (Dyematrex gel blue A column chromatography, ammo nium sulfate fractionation and Dyematrex gel orange column chromatography) were performed according to the method previously described for the purifica tion of human leukocyte M2-PK [22].…”

Fecal pyruvate kinase: A potential new marker for intestinal inflammation in children with inflammatory bowel disease

“…PK exists in two different isotypes in humans. Its tetrameric form (M1) has been found in skeletal muscles, heart and brain, whereas its dimeric form (M2) is present in undifferentiated and proliferating tissues [8]. Increased PK activity in polymorphonuclear neutrophils was documented in patients with polytrauma [9], chronic cardiac failure [10], gastrointestinal tumors [11] and pouchitis [7].…”