Purification of human alpha uterine protein

Immunoregulatory activity of supernatants from shortterm cultures of mouse decidual tissue

“…Cells originally identified in the draining lymph nodes, which produce a soluble non-specific immunosuppressive substance (Clark & McDermott, 1978;Clark, McDermott & Szewczuk, 1980), have also been detected in decidual tissue (Clark & McDermott, 1981) and may constitute a proportion of the small round cells observed in the cultures. The nature and rela¬ tionships of these soluble factors to other decidual tissue products (Umapathysivam & Jones, 1978 ;Bell, 1979b;Rosenberg, Maslar & Riddick, 1980;Sutcliffe, Bolton, Sharp, Nicholson & MacKinnon, 1980) is unknown, but appear to be associated with two protein fractions (Badet et al, 1983).…”

Localization of alpha-uterine protein in human endometrium

“…Rabbit anti-AUP antiserum was prepared in rabbits using purified AUP (Sutcliffe et al, 1980). AUP is a highly stable protein, remaining immunologically detectable after treatment with 3 m caotropic ions, 5 M-guanidine HC1, 8 M-urea or pH [2][3][4][5][6][7][8] (Sutcliffe, Kukulska, Nicholson & Paterson, 1979;Kukulska-Langlands, 1980 (Streefkerk, 1972).…”

“…The protein has also been detected in endometrial tissue from pregnant and non-pregnant women, and at very low concentration in the blood of pregnant women (Sutcliffe, Brock, Nicholson & Dunn, 1978;Sutcliffe, Bolton, Sharp, Nicholson & MacKinnon, 1980). This protein has also been independently identified by Joshi, Ebert & Swartz (1980a) and Joshi, Ebert & Smith (1980b), who called it progestagen-dependent endo¬ metrial protein (PEP) since, in non-pregnant subjects, its synthesis was detected in secretory but not in proliferative endometrium.…”

Characterization of a uterine luminal fluid protein ULF‐250 using N‐terminal microsequencing and RT‐PCR identifies a novel estrogen‐regulated gene in the rat uterus

“…Induction of c-fos gene by 17P-estradiol was observed in the rat uterus [5,6], specially in the luminal and glandular epithelia [7]. Other proteins expressed in the uterus and secreted into the lumen under hormonal influence include the presumptive induced protein [8], alpha uterine protein (AUP) [9,10], the estrogen responsive induced protein (IP) Ill], which was later shown to be a creatine kinase [12], a cat uterine protein that is estrogen dependent (CUPED), which was localized in the endometrial epithelial cells [13], and a mouse lactotransferrin [14]. In addition, estradiol regulates the expression of growth arrest specific (gas) genes [15,16], growth factors and their receptors [17,18].…”