Purification of Histamine Receptor (III) Characterization of Receptor Rich Membrane Fraction of Small Intestinal Smooth Muscle of the Cat

Uchida, Masaatsu K.; Takagi, Keijiro

doi:10.1254/jjp.27.1

Cited by 10 publications

(2 citation statements)

References 26 publications

(12 reference statements)

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“…Histamine receptors were demonstrated to be located on the plasma membrane of several mammalian cells (10,21,22,28,29,43). So far, their presence has been detected by the binding of various cells to histamine which was either insolubilized on THE JOURNAL OF CELL BIOLOGY " VOLUME 93 APRIL 1982 357-364 ©The Rockefeller University Press " 0021-9525/82/04/0357/08 $1 .00 albumin-treated agarose beads (28,46,47), or coupled to bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) (12) or o-phtaldialdehyde (30).…”

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confidence: 99%

Histamine receptors of the microvascular endothelium revealed in situ with a histamine-ferritin conjugate: characteristic high-affinity binding sites in venules.

Heltianu¹,

Simionescu²,

Simiónescu³

1982

The Journal of Cell Biology

135

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Histamine covalently bound to glutaraldehyde-activated ferritin was prepared as either monomers or as small aggregates of -0 .05 to 0.15 p,m Diam, suitable for electron microscopic detection of histamine cellular binding sites. The histamine-ferritin conjugates (MF) maintain the histamine capability to induce the opening of endothelial junctions in venules. To investigate the distribution of histamine receptors in the vascular endothelium, monomers or aggregates of MF were perfused in situ (mice), and various vascular beds, particularly that of the diaphragm, were fixed and processed for electron microscopy . The conjugate was preferentially bound on restricted areas of luminal endothelial cell plasmalemma especially in regions rich in filaments, and near the junctions between endothelial cells. The density of histamine binding sites was characteristically high in venules; it occurred to a much lesser extent in arterioles, veins, and muscular arteries whereas capillaries and aorta showed the lowest values . A similar distribution was obtained after perfusion of H, or H 2 receptor agonists coupled to ferritin (2-pyridylethylamine-ferritin [PF], or 4-methylhistamine-ferritin [MF], respectively) . The binding specificity was assessed through control experiments with either native or activated ferritin or by competition with histamine. The findings suggest that histamine receptors are largely represented in the cell membrane of the vascular endothelium, particularly in venules. Experiments using specific H, and H2 receptor agonists (PF and MF) and antagonists (mepyramine and cimetidine) indicate that the venular endothelium contains mainly H 2 receptors.

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confidence: 99%

Histamine receptors of the microvascular endothelium revealed in situ with a histamine-ferritin conjugate: characteristic high-affinity binding sites in venules.

Heltianu¹,

Simionescu²,

Simiónescu³

1982

The Journal of Cell Biology

135

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“…[15][16][17][18][19][20][21][22][23][24][25][26] It has been demonstrated that histamine receptors are located on the plasma membrane of several mammalian cells. [27][28][29][30][31][32][33] Their presence was so far detected either indirectly, by the binding of various cells to histamine insolubilized on agarose beads,z7.34.35 or visualized at the light microscope level by coupling histamine to bovine serum albumin-fluoresceine isothiocyanatezz or to o-phthaldialdehyde.36 Another indirect way of identifying histamine receptors was by measuring the binding of the antagonist [3H]mepyramine.37 However, the direct detection and the topographical distribution of histamine receptors and their subclasses at the ultrastructural level have not been accomplished yet.…”

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confidence: 99%