Abstract:The liver is primarily composed of hepatocytes and the nonparenchymal cells which include stellate cells, Kupffer cells, sinusoidal endothelial cells and various other cell populations in very low numbers such as transient leukocytes. Cell isolation with high purity is required for many experiments related to the specific biology and biochemistry of these very different cell types. Here, we outline a relatively simple procedure to obtain a high yield of live hepatocytes and sinusoidal endothelial cells. Additi… Show more
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