Purification of Hepatitis B Surface Antigen Using Polyethylene Glycol, Pepsin and Tween 80 1

The present study was undertaken to determine whether there were any differences in the distribution of hepatitis B virus-(HBV) associated serologic markers among hepatitis B surface antigen-(HBsAg) positive first-time and repeat blood donors. The markers examined in samples from 412 newly identified HBsAg-positive donors (254 first-time and 158 repeat) included HBsAg titer and subtype, HBeAg/anti-HBe, and anti-HBc. Repeat donors were more frequently HBeAg-positive (25.9%) than were first-time donors (17.7%). Anti-HBc and anti-HBe were observed more often among first-time (99.6 and 76%) than repeat (91.8 and 60.8%) donors. No differences were found in the mean HBsAg titer nor in the subtype distribution in the two populations. The frequency of HBeAg positivity and the mean HBsAg titer in blood were significantly lower among first-time donors aged 30 or older as compared to those younger than 30. Such age-related tendencies did not occur among the repeat donors. The profiles of HBV makers suggest that the HBsAg-positive first-time donor group consists predominantly of long-term HBsAg carriers who may have acquired HBV at an early age, while the HBsAg-positive repeat donors have newly acquired infections.

Section: Hb V-associated Serologic Markersmentioning

confidence: 98%

Section: Hb V-associated Serologic Markersmentioning

confidence: 99%

Hepatitis‐Associated Markers in the American Red Cross Volunteer Blood Donor Population

Nath

et al. 1982

“…The samples were identified as HBsAg/ad or HBsAg/ay by a counterelectrophoresis (CEP) technique using buffer systems and electropho retic conditions reported by others [10], Antiserum (anti-ad or anti-ay) was elicited in rabbits by immuni zation with HBsAg purified from human sera [16]. For use in CEP, the antisera were made monospecific for d or y by absorption with controlled pore glass coated with HBsAg of the appropriate subtype, according to a method described elsewhere [9].…”

Section: Hbv-associated Serologic Markersmentioning

confidence: 99%

Hepatitis-Associated Markers in the American Red Cross Volunteer Blood Donor Population

Bastiaans¹,

Nath²,

Dodd³

et al. 1982

The present study was undertaken to determine whether there were any differences in the distribution of hepatitis B virus-(HBV) associated serologic markers among hepatitis B surface antigen-(HBsAg) positive first-time and repeat blood donors. The markers examined in samples from 412 newly identified HBsAg-positive donors (254 first-time and 158 repeat) included HBsAg titer and subtype, HBeAg/anti-HBe, and anti-HBc. Repeat donors were more frequently HBeAg-positive (25.9%) than were first-time donors (17.7%). Anti-HBc and anti-HBe were observed more often among first-time (99.6 and 76%) than repeat (91.8 and 60.8%) donors. No differences were found in the mean HBsAg titer nor in the subtype distribution in the two populations. The frequency of HBeAg positivity and the mean HBsAg titer in blood were significantly lower among first-time donors aged 30 or older as compared to those younger than 30. Such age-related tendencies did not occur among the repeat donors. The profiles of HBV markers suggest that the HBsAg-positive first-time donor group consists predominantly of long-term HBsAg carriers who may have acquired HBV at an early age, while the HBsAg-positive repeat donors have newly acquired infections.

“…Antisera (anti-HBs/ayw4; anti-HBs/ ay(/j; anti-HBs/ad; anti-HBs/ay) were produced in laboratory animals against HBsAg purified by the method of Nath et al [23] from the serum of asymptomatic HBV carriers. Goat anti-HBs/ayw4 was produced against HBsAg/ayw4 from a carrier from the Dominican Republic.…”

Section: [1 10]mentioning

confidence: 99%

A New Antigenic Determinant on HBsAg

Bastiaans¹,

Nath²,

Mazzur³

1979

A new antigenic specificity for HBsAg is described, and its usefulness as an epidemiological marker is discussed. This specificity, l , was found in specimens from all geographic areas studied (United States, Latin America, South Africa, the Solomon Islands, and New Guinea). In these areas, l was strongly associated with HBsAg/ay; y-positive, l-negative samples were observed only among specimens from United States volunteer blood donors. This determinant allowed a distinction of HBsAg/ad classes. Thus, the l determinant was detected in 51.4% of HBsAg/adw4, 44.1% of HBsAg/adw(non-w4), and 50% of HBsAg/adr. The association of l and other HBsAg determinants with HBeAg or anti-HBe was also investigated. HBeAg was found to be associated with the presence of the l determinant and with the r determinant. Anti-HBe was associated with the presence of the d and w determinants and with the absence of the l specificity.