Purification of glutathione S‐transferase enzyme from quail liver tissue and inhibition effects of (3aR,4S,7R,7aS)‐2‐(4‐((E)‐3‐(aryl)acryloyl)phenyl)‐3a,4,7,7a‐tetrahydro‐1H‐4,7‐methanoisoindole‐1,3(2H)‐dione derivatives on the enzyme activity

Abstract

The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S-transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88-fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS-PAGE method and showed a single band. In addition, inhibition effects of (3aR,4S,7R,7aS)-2-(4-((E)-3-(aryl)acryloyl)phenyl)-3a,4,7,7a-tetrahydro-1H-4,7methanoisoindole-1,3(2H)-dion derivatives (1a-g) were investigated on the enzyme activity. The inhibition parameters (IC and K values) were calculated for these compounds. IC values of these derivatives (1a-e) were found as 23.00, 15.75, 115.50, 10.00, and 28.75 μM, respectively. K values of these derivatives (1a-e) were calculated in the range of 3.04 ± 0.50 to 131.50 ± 32.50 μM. However, for f and g compounds, the inhibition effects on the enzyme were not found.

In vitro cytotoxic and in vivo antitumoral activities of some aminomethyl derivatives of 2,4‐dihydro‐3H‐1,2,4‐triazole‐3‐thiones—Evaluation of their acetylcholinesterase and carbonic anhydrase enzymes inhibition profiles

“…p ‐Nitrophenyl acetate was used as substrate and transformed by both isoforms to p ‐nitrophenolate ion . For the description of the protein quantity during the purification stages, the Bradford process was used as described previously . The bovine serum albumin was used as a control factor for this definition, which was done at 595 nm .…”

Purification and Characterization of 6-Phosphogluconate Dehydrogenase from Japanese Quail (Coturnix, coturnix japonica) Erythrocytes

“…The method used for the purification of the enzyme was 2ˈ, 5ˈ-ADP Sepharose 4B affinity gel chromatography, which is a very powerful, reliable, easy, economic and a single step purification method that gives very good purification yields with an ability to purify bulk amounts of the enzymes (Temel et al, 2017a,b;Temel and Kocyigit, 2017). These advantages of the method are some of the characteristics of it which make it by comparison to other purification methods to purify different enzymes (Temel et al, 2018;Temel and Bayındır, 2019).…”

Bortezomib ile Kalp Hasarı Oluşturulan Ratlarda Berberinin Oksidatif ve Nitrozatif Stres Üzerine Etkisi

“…Önceki çalışmalarda çeşitli toksik ajanların ROS üretimine bağlı olarak lipid peroksidayonu meydana getirdiğini ve buna bağlı olarak MDA seviyelerinde artışların olduğu bildirilmiştir [40][41][42][43][44]. Artan MDA seviyelerinin oksidatif stresin önemli bir göstergesi olduğunu ve oksidatif stresin de kalp dahil çeşitli dokularda toksisiteye yol açtığı yapılan çalışmalarda kanıtlanmıştır [28,[45][46][47] Biyolojik sistemlerdeki serbest radikallerin ortadan kaldırılması, serbest radikallere karşı önemli savunma sistemleri olarak işlev gören enzimatik ve enzimatik olmayan antioksidanlar tarafından sağlanır [48][49][50][51][52]. İndirgenmiş GSH, ROS'ları (peroksinitrit, hidroksil radikali, lipid peroksil radikali ve H 2 O 2 ) ve diğer serbest radikalleri temizleyen enzimatik olmayan bir antioksidandır.…”