“…Fusion proteins containing either aa 1 to 103 or 104 to 188 of the BNYVV coat protein at the C terminus of a truncated cro-lacl lacZ sequence were obtained by cloning the corresponding cDNA sequences of the viral coat protein gene into the truncated derivative of the pEX3 vector described by Kocken et al (1988). The fusion proteins were expressed in, and purified from, E. coli strain POP 2136 following essentially the procedure of Kocken et al (1988). The pellets obtained after washing the inclusion bodies in 2M-urea, 1 mM-EDTA, 100mM-NaC1, 50 mM-Tris HCI pH 8.0, were resuspended in 9 volumes of 6 M-urea, 0-5~ (v/v) Triton X-100, 10mM-EDTA, 100mM-NaCI, 50mM-Tris-HC1 pH8.0 and centrifuged at 12000g for 20min at 4°C.…”