Purification of Filamentous Viruses and Virus-Induced Noncapsid Proteins

Brunt, A. A.

doi:10.1007/978-1-4684-7038-3_4

Cited by 5 publications

(3 citation statements)

References 147 publications

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“…Of three different types of inclusion, cylindrical inclusion (Cl), amor phous inclusion (AI), and nuclear inclusion, AI and nuclear inclusions have been isolated for a limited number of potyviruses [10,13,14], whereas CIs of many potyviruses have been purified [15,16]. The antigenic proper ties of virus particles and CP have been ex tensively studied [17] and commonly used as criteria in classification.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Serological Comparison of Virus-Coded Proteins of Three Potyviruses Infecting Cucurbitaceous Plants

1990

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Cylindrical inclusion proteins (CIPs), amorphous inclusion proteins (AIPs), and virus particles were partially purified from Cucurbita maxima leaves infected with zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus 2 (WMV2), and the watermelon mosaic virus 1 strain of papaya ringspot virus (PRSV-W). Antisera to these individual antigens, except AIPs of ZYMV and WMV2, were prepared and used to determine with electroblot-ELISA the serological differentiation indices (SDIs) between homologous and heterologous antigens. The average SDIs between ZYMV and WMV2 CIPs, between ZYMV and PRSV-W CIPs, and between WMV2 and PRSV-W CIPs were 1.5, 3.5, and 2.5, respectively. The SDIs between ZYMV and PRSV-W AIPs and between WMV2 and PRSV-W AIPs were 5 and 3, respectively. The average SDIs between ZYMV and WMV2 capsid proteins (CPs), between ZYMV and PRSV-W CPs, and between WMV2 and PRSV-W CPs were 3, 7, and 8, respectively. These data suggest that the serological relationship between ZYMV and WMV2 is much closer than that between PRSV-W and ZYMV or between PRSV-W and WMV2 and that antigenic determinants of CIP and AIP were conserved more than those of CP among the three potyviruses.

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Section: Introductionmentioning

confidence: 99%

Isolation and Serological Comparison of Virus-Coded Proteins of Three Potyviruses Infecting Cucurbitaceous Plants

1990

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“…1a, Table 1). www.nature.com/scientificreports/ MDMV is the most common among virus diseases of maize and can infect more than 200 grass species 14,26,32 . Complete genome sequence information is important to understand the virus evolution based on the entire genome as well as individual genes of a virus and their molecular relationships with other available isolates collected from different regions and countries.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary study of maize dwarf mosaic virus using nearly complete genome sequences acquired by next-generation sequencing

Wijayasekara

Ali

2021

Sci Rep

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Next-generation sequencing is a robust approach to sequence plant virus genomes in a very short amount of time compared to traditional sequencing methods. Maize dwarf mosaic virus (MDMV) is one of the most important plant viruses worldwide and a significant threat to maize production. In this study, we sequenced 19 MDMV isolates (10 from Johnsongrass and 9 from maize) collected in Oklahoma and Missouri during 2017–2019 using Illumina sequencing and determined the genetic diversity. Sequence reads were assembled and 19 nearly complete genome sequences of MDMV isolates were obtained. Phylogenetic analysis based on complete genomes nucleotide and amino acid sequences revealed two main clusters and a close evolutionary relationship among 19 MDMV isolates. Statistical analysis of individual genes for site-specific selection revealed that all genes are under negative selection. The fixation index (FST) analysis of the MDMV isolates revealed no gene flow between the two main phylogenetic clusters, which emphasizes the divergence of MDMV isolates from the USA. Among the USA MDMV isolates, the mean genetic distance (d) and nucleotide diversity ((π) were highest in the P1 gene coding region. This is the first detailed study on the evolutionary relationship of MDMV isolates based on the nearly complete genome analysis from maize and Johnsongrass.

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“…CPMV (figure 1) is an icosahedral plant virus 28.4 nm in diameter, containing a single-stranded RNA [8,9]. The capsid is formed from 60 copies of a large and a small subunit protein.…”

Section: Introductionmentioning

confidence: 99%

Long term storage of virus templated fluorescent materials for sensing applications

et al. 2008

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Wild type, mutant, and chemically modified Cowpea mosaic viruses (CPMV) were studied for long term preservation in the presence and absence of cryoprotectants. Viral complexes were reconstituted and tested via fluorescence spectroscopy and a UV/vis-based RNase assay for structural integrity. When viruses lyophilized in the absence of cryoprotectant were rehydrated and RNase treated, UV absorption increased, indicating that the capsids were damaged. The addition of trehalose during lyophilization protected capsid integrity for at least 7 weeks. Measurements of the fluorescence peak maximum of CPMV lyophilized with trehalose and reconstituted also indicate that the virus remained intact. Microarray binding assays indicated that CPMV particles chemically modified for use as a fluorescent tracer were intact and retained binding specificity after lyophilization in the presence of trehalose. Thus, we demonstrate that functionalized CPMV nanostructures can be stored for the long term, enabling their use in practical sensing applications.

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Purification of Filamentous Viruses and Virus-Induced Noncapsid Proteins

Cited by 5 publications

References 147 publications

Isolation and Serological Comparison of Virus-Coded Proteins of Three Potyviruses Infecting Cucurbitaceous Plants

Isolation and Serological Comparison of Virus-Coded Proteins of Three Potyviruses Infecting Cucurbitaceous Plants

Evolutionary study of maize dwarf mosaic virus using nearly complete genome sequences acquired by next-generation sequencing

Long term storage of virus templated fluorescent materials for sensing applications

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