Purification of extracellular vesicles by size-exclusion chromatography using cross-linked agarose gels: the role of pore size in lipoprotein contamination
Diana Kitka,
Tímea Bebesi,
Anikó Gaál
et al.
Abstract:Extracellular vesicles (EVs) are pivotal in intercellular communication and hold promise for diagnostic and therapeutic applications. Their isolation from complex biological fluids like plasma, however, poses significant challenges. This study examined the impact of pore size in agarose gels on the yield and purity of EVs isolated by Size-Exclusion Chromatography (SEC). We systematically compared the performance of agarose gels with different degrees of crosslinking (Sepharose CL-2B, CL-4B, and CL-6B) using bo… Show more
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