Purification of carp putative eosinophils on metrizamide gradients

Abstract

A protocol for the purification of carp eosinophils on metrizamide gradients is presented. Density gradient centrifugation is a new approach to the study of eosinophils in fish. It provides enriched populations of viable eosinophils necessary for in vitro functional assays. Eosinophils purified from the peripheral blood of carp resemble mammalian eosinophils by light and electron microscopy. They are indistinguishable from carp neutrophils which have a different density and morphology.

Blood cell profile of six Mediterranean mariculture fish species

“…The physiological role of eosinophil cells is not clear, and even their presence in the blood of fish is disputed (Ellis, 1977; Cannon et al., 1980; Hendrick et al., 1986). These cells possess numerous eosinophilic granules although unlike the case in human eosinophils these granules are peroxidase‐negative with an ultrastructure more reminiscent of those in human basophils (Lieschke et al., 2001).…”

“…To date, studies of blood parameters have been carried out either to determine the systematic relationships among certain species (Cameron, 1970; Larsson et al., 1976; Atkinson and Judd, 1978; Putnam and Freel, 1978; Filho et al., 1992) or in relation to the function of the blood cells in the immune response (Hyder et al., 1983; Railo et al., 1985; Hendrick et al., 1986; Parish et al., 1986; Doggett et al., 1987; Thuvander et al., 1987). Predominant groups of interest are the salmonids and cichlids, which are extensively farmed, while the elasmobranches feature in most comparative studies.…”

Comparative hematology of wild Anguilliformes (Muraena helena, L. 1758, Conger conger, L. 1758 and Anguilla anguilla L. 1758)

“…The presence of basophils only in conger and the absence of eosinophils in the analyzed eels is not unusual (Ellis, 1977;Cannon et al, 1980;Hendrick et al, 1986). In the genus Anguilla, some species are recorded to have eosinophils while others lack this cell type (Orecka-Grabida, 1986;Kusuda and Ikeda, 1987;van Ginniken et al, 2005;Ponsen et al, 2009).…”