Purification of carbonic anhydrase isoenzymes by high-performance affinity chromatography and hydrophobic interaction chromatography

Measurement of Carbonic Anhydrase Activity Using a Sensitive Fluorometric Assay

“…Methods that combine the use of pH indicators with stopped flow spectrophotometry have In the catalysis of CO 2 hydration human carbonic anhy-greatly improved the time resolution for analyzing the 3 kinetics of carbonic anhydrase (9,10). Initial rates can preparations, cells were grown synchronously with 12-h light/12-h dark cycles and harvested 5 h into the light be determined by the following relationship:…”

“…Colorimetric assays Carbonic anhydrase (carbonic hydrolyase, E.C. have been developed to follow the esterase activity of 4.2.1.1) catalyzes the reversible dehydration of HCO 0 3 carbonic anhydrase (8,9), but many of the color rein solution:…”

Pectinlyase immobilization on epoxy supports for application in the food processing industry

“…PL was immobilized on epoxy-activated supports by means of a coupling reaction between nucleophilic groups of the enzyme such as amino, mercapto or hydroxy groups and epoxy groups of the matrix. 13 The procedure, which is relatively new, simple and inexpensive, has already been tested in relation to PG.8 In particular, the epoxy-activated supports tested have been: (a) Eupergit C , a copolymer essentially consisting of methacrylamide containing oxirane groups and (b) epoxy-substituted y-Al.15 G . Spagna, P. G .…”

Direct Synthesis of Peptides on Convective Interaction Media Monolithic Columns for Affinity Chromatography

“…They are rigid, macroporous monoliths containing epoxy groups as functional groups that can be activated to allow immobilization of various ligands . CIM monolithic supports have been used for the separation of proteins utilizing ion-exchange, reversed-phase, hydrophobic interaction, and affinity chromatography. ,− Separation of DNA and oligonucleotides , and the use of CIM monolithic supports for enzyme biocatalysis , have also been reported. CIM monolithic supports offer the advantage of high resolution practically unaffected by flow rate, , low back pressure due to high porosity and short column length, low nonspecific binding of biomolecules, and simple handling.…”