Purification of antibodies using the synthetic affinity ligand absorbent MAbsorbent A2P

An automated packed Protein G micro-pipette tip assay for rapid quantification of polyclonal antibodies in ovine serum

“…As alluded to above, one potential challenge when purifying antibodies from serum is the potential for the co-adsorption of the primary impurity (albumin) with the antibody [14,15]. In the case of the Protein G tips, this could cause some albumin to co-elute with the antibody component, thus affecting assay sensitivity.…”

Evaluation of a novel agarose-based synthetic ligand adsorbent for the recovery of antibodies from ovine serum

“…[11]). Synthetics are typically cheaper than recombinant Protein A or Protein G and permit the use of robust cleaning protocols which do not adversely affect binding capacity.…”

“…Polyclonal IgG was purified from ovine serum using a 10 mm Bioprocess TM system (GE Healthcare) within cGMP production facilities, Protherics U.K. Ltd. following the protocols described [7,11]. Frozen bags of ovine serum (Protherics Australasia Pty Ltd.) were thawed at room temperature and filtered using the following filter train: Kleenpak Nova Preflow 'UB' and Kleenpak Nova Supor EKV (Pall Europe Ltd.) to remove particulates and minor precipitates.…”

Affinity Chromatographic Purification of Antibodies

“…Polyclonal antibodies are often considered more efficacious (especially for the treatment of acute illness and medical emergencies) as they can bind to multiple epitopes on the disease-causing agent. Recently, the synthetic Protein A absorbent, MAbsorbent A2P has been shown to be suitable for the purification of polyclonal antibodies (Newcombe et al 2005;Chhatre et al 2007). Unlike many affinity matrices that show IgG subclass specificity, MAbsorbent A2P binds all subclasses of IgG, including IgG3.…”