Purification of Anti-Pernicious Anaemia Factor.

Cellular uptake of cobalamin: Transcobalamin and the TCblR/CD320 receptor

“…A century of clinical and basic science research started with the identification of the cause and treatment of pernicious anemia (114, 115), the isolation of vitamin B12 (116, 117) and elucidation of the structure of cobalamin (118). The advances in biology have led to the identification of pathways, proteins and genes involved (119).…”

Serum Protein Binding of Vitamin B1212

“…More than twenty years intervened between the discovery of the effectiveness of raw liver and the eventual isolation of the active principle by two groups, working independently in the United States (Rickes et al 1948) and Great Britain (Lester, Smith & Parker 1948). The initial clinical studies (West 1948) showed that the isolated material was effective in eliciting a therapeutic response in pernicious anaemia patients.…”

“…Although the clinical effectiveness of vitamin B12 has been recognized for more than forty years (Minot & Murphy 1926), and its chemical structure known for twenty years (Rickes et al 1948, Lester Smith & Parker 1948), there still remains much to be learned about the transport of vitamin BI2 within the human body. Radioactive isotopes of vitamin BIZ have been available for biological use for sixteen years (Heinle et al 1952), but they have not led to rapid elucidation of the details of the transport and metabolism of the vitamin for several reasons.…”

Vitamin B12

“…Vitamin B12, or cobalamin, was the last vitamin to be isolated 1,2 . It has the most complex structure and largest molecular weight (1335 Da) of all the vitamins.…”